2017, Number 2
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Arch Neurocien 2017; 22 (2)
Cytotoxicity of metal nanoparticles in brain cell cultures
Manzo RMI, Castro GPB, Agarwal V, Mora LS
Language: Spanish
References: 40
Page: 13-23
PDF size: 329.67 Kb.
ABSTRACT
Nanoparticles (NP) have been investigated for their miniscule size and their
remarkable to reach specific sites; these materials have been studied to design
harmless nano-vectors. However, there are insufficient studies about the effects of
the exposure of different NP are able to cross the blood-brain barrier (BBB), over
the cells in this tissue.
Aim: To determine the cellular toxicity in the presence of three different nanoparticles
of metallic origin in brain in vitro.
Materials and methods: MTT assay and morphological analysis were performed
on cultured glia and neurons after treatment with Silica (NPSiO
2), gold-coated with
Silicon (NPAu@Si) and silver (NPAg) NP at 10, 50 100 and 250 µg/ml.
Results: Cerebral cells were affected in their viability and morphology after
treatment with NPAg. NPSiO
2 treated cells showed a decrease in their viability but
not as severe as with NPAg. For NPAu@Si, a slightly higher resistance was observed
even at the highest concentration.
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