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Instituto Nacional de Salud Pública

2018, Number 1

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salud publica mex 2018; 60 (1)

High relative abundance of firmicutes and increased TNF-α levels correlate with obesity in children

Orbe-Orihuela YC, Lagunas-Martínez A, Bahena-Román M, Madrid-Marina V, Torres-Poveda K, Flores-Alfaro E, Méndez-Padrón A, Díaz-Benítez CE, Peralta-Zaragoza O, Antúnez-Ortiz D, Cruz M, Burguete-García AI

Language: English
References: 39
Page: 5-11
PDF size: 293.62 Kb.


ABSTRACT

Objective. To investigate the correlation among pro- or anti-inflammatory cytokines and the two main gut microbiota phyla in obese children. Materials and methods. Anthropometric data were obtained from 890 children under 14 years old to determine the degree of obesity. Serum cytokine concentration was measured by ELISA. Relative abundance of gut microbiota in feces was evaluated by quantitative Real-Time PCR assays. Results. Anthropometric and biochemical parameters were statistically higher in overweight /obese children than in lean ones. Increased TNF-α levels were found in obese children that also have a high relative abundance of Firmicutes. Conclusions. Obese children have a high relative abundance of Firmicutes that correlates with increased levels of TNF-α. This is the first study that shows a relation between Firmicute abundance and TNF-α serum concentration in obese children.


REFERENCES

  1. World Health Organization. Obesity: preventing and managing the global epidemic. Report of a WHO consultation. World Health Organ Tech Rep Ser 2000;894:i-xii,1-253.

  2. Rivera JÁ, de Cosio TG, Pedraza LS, Aburto TC, Sánchez TG, Martorell R. Childhood and adolescent overweight and obesity in Latin America: a systematic review. Lancet Diabetes Endocrinol 2014;2(4):321-332. https:// doi.org/10.1016/S2213-8587(13)70173-6

  3. Romero-Martínez M, Shamah-Levy T, Franco-Núñez A, Villalpando S, Cuevas-Nasu L, Gutiérrez JP, et al. National Health and Nutrition Survey 2012: design and coverage. Salud Publica Mex 2013;55(2):S332-S340. http://dx.doi.org/10.21149/spm.v55s2.5132

  4. Giles-Corti B, Macintyre S, Clarkson JP, Pikora T, Donovan RJ. Environmental and lifestyle factors associated with overweight and obesity in Perth, Australia. Am J Health Promot 2003;18(1):93-102. https://doi. org/10.4278/0890-1171-18.1.93

  5. Kang S, Denman SE, Morrison M, Yu Z, Dore J, Leclerc M et al. Dysbiosis of fecal microbiota in Crohn’s disease patients as revealed by accustom phylogenetic microarray. Inflamm Bowel Dis 2010;16(12):2034-2042. https://doi.org/10.1002/ibd.21319

  6. Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, et al. Diversity of the human intestinal microbial flora. Science 2005;308(5728):1635-1638. https://doi.org/10.1126/science.1110591

  7. Ley RE, Turnbaugh PJ, Klein S, Gordon JI. Microbial ecology: human gut microbes associated with obesity. Nature 2006;444(7122):1022-1023. https://doi.org/10.1038/4441022a

  8. Turnbaugh PJ, Hamady M, Yatsunenko T, Cantarel BL, Duncan A, Ley RE, et al. A core gut microbiome in obese and lean twins. Nature 2009;457(7228):480-484. https://doi.org/10.1038/nature07540

  9. Abdallah-Ismail N, Ragab SH, Abd-Elbaky A, Shoeib AR, Alhosary Y, Fekry D. Frequency of Firmicutes and Bacteroidetes in gut microbiota in obese and normal weight Egyptian children and adults. Arch Med Sci 2011;7(3):501-507. https://doi.org/10.5114/aoms.2011.23418

  10. Bervoets L, van Hoorenbeeck K, Kortleven I, Van Noten C, Hens N, Vael C, et al. Differences in gut microbiota composition between obese and lean children: a cross-sectional study. Gut Pathog 2013;5(1):10. https:// doi.org/10.1186/1757-4749-5-10

  11. Estrada-Velasco BI, Cruz M, García-Mena J, Valladares-Salgado A, Peralta- Romero J, Guna-Serrano M de L, et al. Childhood obesity is associated to the interaction between firmicutes and high energy food consumption. Nutr Hosp 2015;31(3):1074-1081.

  12. Popko K, Gorska E, Stelmaszczyk-Emmel A, Plywaczewski R, Stoklosa A, Gorecka D, et al. Proinflamatory cytokines IL-6 and TNF-α and the development of inflammation in obese subjects. Eur J Med Res 2010;15(2):120-122.

  13. Jung SH, Park HS, Kim KS, Choi WH, Ahn CW, Kim BT, et al. Effect of weight loss on some serum cytokines in human obesity: increase in IL-10 after weight loss. J Nutr Biochem 2008;19(6):371-375. https://doi. org/10.1016/j.jnutbio.2007.05.007

  14. Gutiérrez JP, Rivera-Dommarco J, Shamah-Levy T, Villalpando- Hernández S, Franco A, Cuevas-Nasu L, et al. Encuesta Nacional de Salud y Nutrición 2012. Resultados Nacionales. Cuernavaca, México: Instituto Nacional de Salud Pública, 2012.

  15. De Onis M, Onyango A, Borghi E, Siyam A, Nishida Ch, Siekmann J. Development of a WHO growth reference for schoolaged children and adolescents. Bull World Health Organ 2007;85:660-667. https://doi. org/10.2471/BLT.07.043497

  16. Bacchetti De Gregoris T, Aldred N, Clare AS, Burgess JG. Improvement of phylum- and class-specific primers for real-time PCR quantification of bacterial taxa. J Microbiol Methods 2011;86(3):351-356. https://doi. org/10.1016/j.mimet.2011.06.010

  17. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc 2008;3(6):1101-1108. https://doi.org/10.1038/ nprot.2008.73

  18. DiBaise JK, Zhang H, Crowell MD, Krajmalnik-Brown R, Decker GA, Rittmann BE. Gut Microbiota and its possible relationship with obesity. Mayo Clin Proc 2008;83(4):460-469. https://doi.org/10.4065/83.4.460

  19. Ley RE, Bäckhed F, Turnbaugh P, Lozupone CA, Knight RD, Gordon JI. Obesity alters gut microbial ecology. Proc Natl Acad Sci USA 2005;102(31):11070-11075. https://doi.org/10.1073/pnas.0504978102

  20. Sepp E, Loivukene K, Julge K, Voor T, Mikelsaar M. The association of gut microbiota with body weight and body mass index in preschool children of Estonia. Microb Ecol Health Dis 2013;24:19231. https://doi. org/10.3402/mehd.v24i0.19231

  21. Schwiertz A, Taras D, Schäfer K, Beijer S, Bos NA, Donus C, et al. Microbiota and SCFA in lean and overweight healthy subjects. Obesity (Silver Spring) 2010;18(1):190-195. https://doi.org/10.1038/oby.2009.167

  22. Patil DP, Dhotre DP, Chavan SG, Sultan A, Jain DS, Lanjekar VB, et al. Molecular analysis of gut microbiota in obesity among Indian individuals. J Biosci 2012;37(4):647-657. https://doi.org/10.1007/s12038-012-9244-0

  23. Escobar JS, Klotz B, Valdes BE, Agudelo GM. The gut microbiota of Colombians differs from that of Americans, Europeans and Asians. BMC Microbiol 2014;14:311. https://doi.org/10.1186/s12866-014-0311-6

  24. Fernandes J, Su W, Rahat-Rozenbloom S, Wolever TM, Comelli EM. Adiposity, gut microbiota and faecal short chain fatty acids are linked in adult humans. Nutr Diabetes 2014;4:e121. https://doi.org/10.1038/nutd.2014.23

  25. Kocelak P, Zak-Golab A, Zahorska-Markiewicz B, Aptekorz M, Zientara M, Martirosian G, et al. Resting energy expenditure and gut microbiota in obese and normal weight subjects. Eur Rev Med Pharmacol Sci 2013;17(20):2816-2821.

  26. Turnbaugh PJ, Ley ER, Mahowald MA, Magrini V, Mardis ER, Gordon JI. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 2006;444(7122):1027-1031. https://doi.org/10.1038/ nature05414

  27. Mariat D, Firmesse O, Levenez F, Guimarăes V, Sokol H, Doré J, et al. The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age. BMC Microbiol 2009;9:123. https://doi.org/10.1186/1471-2180-9-123

  28. Xu P, Li M, Zhang J, Zhang T. Correlation of intestinal microbiota with overweight and obesity in Kazakh school children. BMC Microbiol 2012;12:283. https://doi.org/10.1186/1471-2180-12-283

  29. Ishikawa E, Matsuki T, Kubota H, Makino H, Sakai T, Oishi K, et al. Ethnic diversity of gut microbiota: species characterization of Bacteroides fragilis group and genus Bifidobacterium in healthy Belgian adults, and comparison with data from Japanese subjects. J Biosci Bioeng 2013;116(2):265- 270. https://doi.org/10.1016/j.jbiosc.2013.02.010

  30. Yasir M, Angelakis M, Bibi F, Azhar EI, Bachar D, Lagier JC, et al. Comparison of the gut microbiota of people in France and Saudi Arabia. Nutr Diabetes 2015;5:e153. https://doi.org/10.1038/nutd.2015.3

  31. William A Walters, Zech Xu, Rob Knight. Meta-analyses of human gut microbes associated with obesity and IBD. FEBS Lett 2014:588(22):4223- 4233. https://doi.org/10.1016/j.febslet.2014.09.039

  32. De Filippo C, Cavalieri D, Di Paola M, Ramazzotti M, Poullet JB, Massart S, et al. Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa. Proc Natl Acad Sci USA 2010;107(33):14691-14696. https://doi.org/10.1073/pnas.1005963107

  33. Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, et al. Human gut microbiome viewed across age and geography. Nature 2012;486(7402):222-227. https://doi.org/10.1038/nature11053

  34. Nakayama J, Watanabe K, Jiang J, Matsuda K, Chao SH, Haryono P, et al. Diversity in gut bacterial community of school-age children in Asia. Sci Rep 2015;5:8397. https://doi.org/10.1038/srep08397

  35. La-Ongkham O, Nakphaichit M, Leelavatcharamas V, Keawsompong S, Nitisinprasert S. Distinct gut microbiota of healthy children from two different geographic regions of Thailand. Arch Microbiol 2015;197(4):561- 573. https://doi.org/10.1007/s00203-015-1089-0

  36. Bäckhed F. Programming of host metabolism by the gut microbiota. Ann Nutr Metab 2011;58(2):44-52. https://doi.org/10.1159/000328042

  37. Osborn O, Olefsky J. The cellular and signaling networks linking the immune system and metabolism in disease. Nat Med 2012;18(3):1-10. https://doi.org/10.1038/nm.2627

  38. Cani PD, Bibiloni R, Knauf C, Waget A, Neyrinck AM, Delzene NM, Burcelin R. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 2008;57(6):1470-1481. https://doi.org/10.2337/db07-1403

  39. 39 . Cavalcante-Silva LH, Galvão JG, da Silva JS, de Sales-Neto JM, Rodrigues-Mascarenhas S. Obesity-Driven Gut Microbiota Inflammatory Pathways to Metabolic Syndrome. Front Physiol 2015;6:341. https://doi. org/10.3389/fphys.2015.00341




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