>Gaceta Médica de México
>Year 2018, Issue 1
Jakez-Ocampo J, Paulín-Vera CM, Rivadeneyra-Espinoza L, Gómez-Martín D, Carrillo-Maravilla E, Lima G, Vargas-Rojas MI, Pérez-Romano B, Calva-Cevenini G, García-Carrasco M, Ruiz-Argüelles A, Llorente L
Genes del receptor variable beta de células T en células circulantes de pacientes con lupus eritematoso generalizado y sus familiares sanos
Gac Med Mex 2018; 154 (1)
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Objective: We investigated the proportion of Vβ T cell receptor (TCR) gene expression in peripheral CD3+ lymphocytes in
familial and non-familial systemic lupus erythematosus (SLE) patients. Method: The Vβ TCR repertoire was studied in 14
families in which several members had SLE. The Vβ TCR usage in SLE patients (n = 27) was compared with that in healthy
members of these multiplex families (n = 47), in 37 sporadic SLE patients who had no relatives with SLE, and in 15 healthy
unrelated controls. Vβ TCR repertoire expression was studied by multiparameter flow cytometry with the use of an array of 24
different Vβ TCR gene family-specific monoclonal antibodies. Results: We found the same Vβ TCR expression profile in the comparisons between sporadic SLE and familial SLE cases and healthy relatives, which included increased expression of Vβ
5.2, Vβ 11 and Vβ 16, and lower expression of Vβ 3, Vβ 4, Vβ 7.1 and Vβ 17. Interestingly, solely Vβ 17 was differentially
expressed among sporadic and familial SLE. Also, increased expression of Vβ 9 was the hallmark among familial SLE (cases
and healthy relatives) in comparison to controls. Conclusion: These results highlight the notion that the final profile of the Vβ
TCR repertoire seen in familial and non-familial SLE seems to arise from the interaction of genetic, environmental, and immunoregulatory
factors. Furthermore, it may contribute to the immunologic abnormalities affecting relatives of SLE patients.
||Vβ T cell receptor repertoire, Systemic lupus erythematosus, Sporadic lupus erythematosus, Familiar systemic lupus erythematosus.
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>Gaceta Médica de México
>Year 2018, Issue 1