Hernández DR, Sanabria NJG, Zaldívar GI, Llanuch LM

Language: Spanish
References: 0
Page: 208-215
PDF size: 248.07 Kb.

ABSTRACT

Introduction: the consumption in high doses of vitamin-A at a minimum teratogenic dose could be a possible cause of histological alterations of bones during intrauterine growth.
Objective: to assess the effect of hypervitaminosis A induced in rats on the long bones of offspring.
Method: an experimental study was conducted on pregnant Sprague Dawley rats: two groups with three animals each: negative control and cases with VA dose 50 μg / g during the first 16 days of pregnancy between September 2015 and February 2016. The right femurs of all rats were taken (n = 12 in each group) 2 hours after birth. The bones were decalcified, processed in paraffin and stained with haematoxylin / eosin. Longitudinal section morphometry of the right femur was performed at the midpoint of the diaphysis of the thickness of the periosteum, endosteum, cortical and total of the diaphysis. The qualitative variables were summarized by frequencies and the quantitative using the mean and standard deviation. The comparisons were verified by X2 and Student's-T tests at 95 % certainty.
Results: thickness of the periosteum and endosteum, cortex thickness, and diaphysis of the femur showed highly significant decreases (p <0.0001) when comparing the means between the study and control groups. Increased endochondral cartilage and dilation of medullar vessels were more frequent among cases (p <0.0001).
Conclusions: hypervitaminosis A during pregnancy in Sprague Dawley rats reduces the size of the femur in offspring.