Procedure optimization for concentration and detection of protozoa and helminths in great volumes of water

Sandra Ríos Tobón; Juliana López Jiménez; Laura Francisca Campo Polanco; Ruth Marina Agudelo Cadavid; Lina Andrea Gutiérrez Builes

2018, Number 1

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Rev Cubana Med Trop 2018; 70 (1)

Procedure optimization for concentration and detection of protozoa and helminths in great volumes of water

Ríos TS, López JJ, Campo PLF, Agudelo CRM, Gutiérrez BLA

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Language: English
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Page: 71-77
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ABSTRACT

Feces-contaminated water is a vehicle of transmission of potentially pathogenic microorganisms responsible for illnesses that represent main causes of death worldwide. A protocol for detection of intestinal parasites in great volumes of water was optimized. It includes: membrane filtration, mechanical agitation with detergent, centrifugation, chemical concentration with Mini Parasep® and microscopic examination. From samples of feces-contaminated water containing parasitic forms, a total recovery percentage of 85.7 % of parasites was achieved after tests. This procedure provides a useful alternative method that could be subjected to validation as a routine methodology in the diagnosis of microbiological water quality.