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2018, Number 2

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Rev Med UV 2018; 18 (2)

Pre-processing temperature and time: essential factors for cellular performance from Wharton’s gelatin

Duarte RJM, Muñoz MN, Ávila J, Riveros Á, Franco D, Ávila PLM

Language: Spanish
References: 23
Page: 7-24
PDF size: 1473.38 Kb.


ABSTRACT

A quasi-experimental study was carried out, where 25 umbilical cords (UC) were collected in transport solution of which only nine (9) fulfilled the inclusion criteria. The size of the cords was greater than 30 cm with a diameter of ≥ 1.5 cm; these were cut into 15 fragments of approximately 2 cm and exposed to different temperatures (10 ° C, 22 ° C and 32 ° C) for 6, 12, 24, 48 and 72 hours. It was obtained 600 mgs of Wharton’s gelatin (WJ) from each fragment. Cellularity and viability were analyzed with trypan blue staining. The fragments maintained at 10 ° C presented an average viability of 85.6% ± 6.8 at 6 hours, with a loss of viability at 72 hours of 13.6% (p = 0.001). At 22 ° C the viability average was 84.3% ± 4.2 with a loss of viability at 72 hours of 27.4% (p ‹0.001). At 32°C 83.6% ± 4.6 with a decrease of 60.4% at 72 hours (p ‹0.001). With respect to the number of cells / mg of WJ, at 22 ° C an average of 167 cells / mg ± 36.5 was presented at 6 hours with a cell loss at 72 hours of 112 cells / mg (p = 0.001). ). At 32 ° C 139 cel./mg ± 73.7, with a loss of 98 cel./mg at 72 hours (p = 0.003). The cellularity was not statistically significant at 10 ° C between the different times (p = 0.931). The data found in this study indicate that the ideal temperature for the preservation and transport of UC tissue is 10 ° C, with a maximum conservation time of 24 hours.


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