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Anales de la Sociedad Mexicana de Oftalmología y Archivos de la Asociación Para Evitar la Ceguera en México

2018, Number 1

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Rev Mex Oftalmol 2018; 92 (1)

Evaluation of the expression of T helper lymphocytes markers associated with Th1, Th2, Th17 and Treg cells in primary pterygium biopsies

Díaz-Palomera CD, Rosales-Díaz R, Martínez-Rizo AB, Alvarado-Castillo B, Santos A, Navarro-Partida J

Language: Spanish
References: 28
Page: 26-31
PDF size: 233.60 Kb.


ABSTRACT

Introduction: Various pro-inflammatory cytokines and growth factors have been implicated in the pathogenesis of pterygium (Pt); however, the effect of cytokines produced by helper T lymphocytes (Th) has been poorly studied. To date, only interleukin 4 (IL-4) from Th lymphocytes has been linked to Pt recurrence. Therefore, this study aimed to evaluate the expression of Th cytokines and Th transcription factors in primary Pt. Methods: Pt biopsies were obtained from 28 eyes of 28 Mexican patients undergoing primary excision of Pt with conjunctival autograft. Conjunctival biopsies of 8 patients undergoing cataract surgery were used as the control group. Gene expression of Th cytokines - interferon gamma (IFN-γ), IL-13, IL-17, IL-10-, as well as gene expression of Th transcription factors T-bet, GATA3, Foxp3 and RORγt, was analyzed by real-time RT-PCR. Results: We found decreased expression of markers T-bet, GATA3, RORγt, IFN- γ, IL-13 and IL-10 in Pt. We found a sixfold increase in the expression of IL-17 and Foxp3 in Pt compared to healthy conjunctiva (p ‹ 0.0001); this increase was dependent on Pt size. Conclusions: The increased expression of IL-17 and Foxp3 in Pt samples suggests the presence of IL-17+ Foxp3+ Th lymphocytes, a subset of the Th population with the ability to suppress T cell proliferation and promote tumor progression. Therefore, IL17+ Foxp3+ Th cells may be involved in the occurrence and growth of Pt.


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