medigraphic.com

2019, Number 1

<< Back Next >>

Vet Mex 2019; 6 (1)

Mycobacterium tuberculosis and Mycobacterium bovis derived proteins induce caspase-independent apoptosis in bovine macrophages

Maciel RA, Flores VS, Jiménez VS, Catalan BO, Espitia PC, Moran J, Arriaga-Pizano L, Benítez GA, Alfonseca SE, Gutiérrez-Pabello JÁ

Language: English/Spanish
References: 24
Page: 1-12
PDF size: 1143.93 Kb.


ABSTRACT

Species of the genus Mycobacterium are capable of inducing cell apoptosis. Infected cells with M. tuberculosis undergo apoptosis through a caspase-dependent pathway. We have previously shown that whole Mycobacterium bovis cells and derived crude protein extracts were able to trigger apoptosis through a caspase independent mechanism. However, the identity of the protein or protein fractions capable of inducing apoptosis has not been determined. In this study, bovine macrophages were incubated in the presence of Mycobacterium tuberculosis recombinant proteins and Mycobacterium bovis protein extracts to identify proteins capable of inducing apoptosis. A subgroup of proteins and protein extracts from strains of the Mycobacterium tuberculosis complex were shown to induce DNA fragmentation in bovine macrophages through a caspase independent pathway. Our findings suggest that bovine macrophages may elicit a different response to mycobacterium infection than that displayed by murine or human cells.


REFERENCES

  1. Michel AL, Muller B, van Helden PD. Mycobacterium bovis at the animal-human interface: a problem, or not? Veterinary microbiology. 2010;140(3-4):371-81. Epub 2009/09/24. doi: 10.1016/j.vetmic.2009.08.029.

  2. Caminiti A, Pelone F, LaTorre G, De Giusti M, Saulle R, Mannocci A, et al. Control and eradication of tuberculosis in cattle: a systematic review of economic evidence. The Veterinary record. 2016;179(3):70-5. Epub 2016/07/17. doi: 10.1136/vr.103616.

  3. Sakamoto K. The pathology of Mycobacterium tuberculosis infection. Veterinary pathology. 2012;49(3):423-39. Epub 2012/01/21. doi: 10.1177/0300985811429313.

  4. Cassidy JP. The pathogenesis and pathology of bovine tuberculosis with insights from studies of tuberculosis in humans and laboratory animal models. Veterinary microbiology. 2006;112(2-4):151-61. Epub 2005/11/29. doi: 10.1016/j. vetmic.2005.11.031.

  5. Rohde K, Yates RM, Purdy GE, Russell DG. Mycobacterium tuberculosis and the environment within the phagosome. Immunological reviews. 2007;219:37-54. Epub 2007/09/14. doi: 10.1111/j.1600-065X.2007.00547.x.

  6. Molloy A, Laochumroonvorapong P, Kaplan G. Apoptosis, but not necrosis, of infected monocytes is coupled with killing of intracellular bacillus Calmette-Guerin. The Journal of experimental medicine. 1994;180(4):1499-509. Epub 1994/10/01.

  7. Behar SM, Martin CJ, Booty MG, Nishimura T, Zhao X, Gan HX, et al. Apoptosis is an innate defense function of macrophages against Mycobacterium tuberculosis. Mucosal immunology. 2011;4(3):279-87. Epub 2011/02/11. doi: 10.1038/mi.2011.3.

  8. Lee J, Hartman M, Kornfeld H. Macrophage Apoptosis in Tuberculosis. Yonsei Medical Journal. 2009;50(1):1-11. doi: 10.3349/ymj.2009.50.1.1.

  9. Wu H, Che X, Zheng Q, Wu A, Pan K, Shao A, et al. Caspases: a molecular switch node in the crosstalk between autophagy and apoptosis. International journal of biological sciences. 2014;10(9):1072-83. Epub 2014/10/07. doi: 10.7150/ ijbs.9719.

  10. Esquivel-Solis H, Vallecillo AJ, Benitez-Guzman A, Adams LG, Lopez-Vidal Y, Gutierrez- Pabello JA. Nitric oxide not apoptosis mediates differential killing of Mycobacterium bovis in bovine macrophages. PloS one. 2013;8(5):e63464. Epub 2013/05/22. doi: 10.1371/journal.pone.0063464.

  11. Divangahi M, Behar SM, Remold H. Dying to live: how the death modality of the infected macrophage affects immunity to tuberculosis. Advances in experimental medicine and biology. 2013;783:103-20. Epub 2013/03/08. doi: 10.1007/978-1-4614-6111-1_6.

  12. Chen M, Gan H, Remold HG. A mechanism of virulence: virulent Mycobacterium tuberculosis strain H37Rv, but not attenuated H37Ra, causes significant mitochondrial inner membrane disruption in macrophages leading to necrosis. Journal of immunology (Baltimore, Md : 1950). 2006;176(6):3707-16. Epub 2006/03/07.

  13. Gutierrez-Pabello JA, McMurray DN, Adams LG. Upregulation of thymosin beta- 10 by Mycobacterium bovis infection of bovine macrophages is associated with apoptosis. Infection and immunity. 2002;70(4):2121-7. Epub 2002/03/16.

  14. Vega-Manriquez X, Lopez-Vidal Y, Moran J, Adams LG, Gutierrez-Pabello JA. Apoptosis-inducing factor participation in bovine macrophage Mycobacterium bovis-induced caspase-independent cell death. Infection and immunity. 2007;75(3):1223-8. Epub 2006/12/13. doi: 10.1128/iai.01047-06.

  15. Fifis T, Costopoulos C, Radford AJ, Bacic A, Wood PR. Purification and characterization of major antigens from a Mycobacterium bovis culture filtrate. Infection and immunity. 1991;59(3):800-7.

  16. Flores-Villalva S, Rogriguez-Hernandez E, Rubio-Venegas Y, Canto-Alarcon JG, Milian-Suazo F. What Can Proteomics Tell Us about Tuberculosis? Journal of microbiology and biotechnology. 2015;25(8):1181-94. Epub 2015/03/05. doi: 10.4014/jmb.1502.02008.

  17. Ciaramella A, Martino A, Cicconi R, Colizzi V, Fraziano M. Mycobacterial 19-kDa lipoprotein mediates Mycobacterium tuberculosis-induced apoptosis in monocytes/ macrophages at early stages of infection. Cell death and differentiation. 2000;7(12):1270-2. Epub 2001/03/29. doi: 10.1038/sj.cdd.4400761.

  18. Choi HH, Shin DM, Kang G, Kim KH, Park JB, Hur GM, et al. Endoplasmic reticulum stress response is involved in Mycobacterium tuberculosis protein ESAT-6-mediated apoptosis. FEBS letters. 2010;584(11):2445-54. Epub 2010/04/27. doi: 10.1016/j.febslet.2010.04.050.

  19. Basu S, Pathak SK, Banerjee A, Pathak S, Bhattacharyya A, Yang Z, et al. Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha. The Journal of biological chemistry. 2007;282(2):1039-50. Epub 2006/11/11. doi: 10.1074/jbc.M604379200.

  20. Sanchez A, Espinosa P, Esparza MA, Colon M, Bernal G, Mancilla R. Mycobacterium tuberculosis 38-kDa lipoprotein is apoptogenic for human monocyte-derived macrophages. Scandinavian journal of immunology. 2009;69(1):20-8. Epub 2009/01/15. doi: 10.1111/j.1365-3083.2008.02193.x.

  21. Sohn H, Kim JS, Shin SJ, Kim K, Won CJ, Kim WS, et al. Targeting of Mycobacterium tuberculosis heparin-binding hemagglutinin to mitochondria in macrophages. PLoS pathogens. 2011;7(12):e1002435. Epub 2011/12/17. doi: 10.1371/journal.ppat.1002435.

  22. Bulut Y, Michelsen KS, Hayrapetian L, Naiki Y, Spallek R, Singh M, et al. Mycobacterium tuberculosis heat shock proteins use diverse Toll-like receptor pathways to activate pro-inflammatory signals. The Journal of biological chemistry. 2005;280(22):20961-7. Epub 2005/04/06. doi: 10.1074/jbc.M411379200.

  23. Anand PK, Anand E, Bleck CK, Anes E, Griffiths G. Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria. PloS one. 2010;5(4):e10136. Epub 2010/04/21. doi: 10.1371/journal.pone.0010136.

  24. Broker LE, Kruyt FA, Giaccone G. Cell death independent of caspases: a review. Clinical cancer research : an official journal of the American Association for Cancer Research. 2005;11(9):3155-62. Epub 2005/05/04. doi: 10.1158/1078- 0432.ccr-04-2223.




2020     |     www.medigraphic.com