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ISSN 2992-8036 (Electronic)
ISSN 2306-4102 (Print)
Órgano Oficial del Colegio Mexicano de Ortopedia y Traumatología

2004, Number s1

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Acta Ortop Mex 2004; 18 (s1)

Measuring the bone mineral contents in lumbar L2-L4 vertebrae of Long Evans female rats by dual beam X-ray absorptiometry and chemical analysis

Quintana-Armenta A, Ruiz-Rosano L, Valdez-Mijares R, Diez-García MP, Isoard-Acosta F, Herrero-Bervera B, Franco-Bourland RE

Language: English
References: 10
Page: 2-6
PDF size: 72.19 Kb.


ABSTRACT

Dual beam X-ray absorptiometry is a non invasive method to learn about the status of bone mass in rats. Using a Hologic QDR-2000 densitometer programmed and calibrated to study lumbar L2-L4 vertebrae in rats, we assessed the precision and accuracy of mineral bone density (MBD) and bone mineral contents (BMC) measured in a group of 14 Long Evans female rats, 22 weeks old, weighing 290 to 330 grams. Three consecutive measures were made under anesthesia with rat repositioning. MBD and BMC were (mean ± SD): 175.20 ± 2.00 mg/cm2 and 450.60 ± 18-00 mg respectively with intra-assay group variance coefficients of 1.5 ± 0.20% and 4.12 ± 1.95% (mean ± SD) respectively showing good precision. Spectrophotometry analysis of calcium, magnesium and phosphorus contents in the HNO3 digestion product of vertebrae L2-L4 of the same rats were (in mg, mean ± SD): 131.21 ± 6.93, 2.22 ± 0.17 and 55.77 ± 3.45 respectively with linear correlation coefficients (r) against their BMC of 0.52, 0.33 and 0.47 (p › 0.05) (accuracy measure) suggesting that BMC measures are approximations and do not replace chemical analysis.


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