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ISSN 1561-3011 (Electronic)

2018, Number 3

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Rev Cubana Invest Bioméd 2018; 37 (3)

Effect of a nutraceutical product from Pleurotus mushroom on the immune response of immunocompetent mice of both sexes

Llauradó G, Morris HJ, Lebeque Y, Guilarte A, Guiérrez A, Beltrán Y

Language: Spanish
References: 45
Page: 1-17
PDF size: 253.73 Kb.


ABSTRACT

Introduction: The edible mushrooms Pleurotus are currently considered as a source of obtaining nutraceuticals and mycochemicals with biotherapeutic potential. Although several studies highlight the immunomodulatory effects of biopreparations derived from this genus in immunodeficient mice, there are still aspects not clarified in preclinical studies regarding the influence of sex on the immune function of immunocompetent mice.
Objectives: To evaluate the effect of a dry biopreparation derived from fruiting bodies of Pleurotus sp. on metabolic parameters and the immune response of immunocompetent mice of both sexes.
Methods: The administration of the nutraceutical biopreparation was carried out for 14 days orally (1000 mg / kg) and serum hematological and biochemical markers were subsequently determined and the innate and adaptive immune response was evaluated by counting phagocytic cells and delayed hypersensitivity response, respectively.
Results: The biopreparation increased the total leukocyte count, with a polarized response that showed significant differences in lymphocyte and polymorphonuclear populations in male and female mice, respectively. We also observed modulating effects at the splenic cellular level and an increase in the number of peritoneal macrophages in the treated females. Also, although no significant results were detected in the levels of hemoglobin and total serum proteins, a differential behavior was observed that favors the synthesis of the globulin fraction in the females that received the nutraceutical. The supplement also stimulated the cellular immune response in vivo in male mice.
Conclusions: The present study provides new evidence of the nutraceutical and immunomodulatory effect of a Pleurotus sp. in immunocompetent mice of both sexes. This suggests the estimation of immunomodulation markers both in preclinical studies and in nutritional interventions in humans and also consider the influence of sex in these studies.


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