2018, Number 3
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Rev Cubana Invest Bioméd 2018; 37 (3)
Effect of a nutraceutical product from Pleurotus mushroom on the immune response of immunocompetent mice of both sexes
Llauradó G, Morris HJ, Lebeque Y, Guilarte A, Guiérrez A, Beltrán Y
Language: Spanish
References: 45
Page: 1-17
PDF size: 253.73 Kb.
ABSTRACT
Introduction: The edible mushrooms Pleurotus are currently considered as a source
of obtaining nutraceuticals and mycochemicals with biotherapeutic potential. Although
several studies highlight the immunomodulatory effects of biopreparations derived
from this genus in immunodeficient mice, there are still aspects not clarified in
preclinical studies regarding the influence of sex on the immune function of
immunocompetent mice.
Objectives: To evaluate the effect of a dry biopreparation derived from fruiting
bodies of Pleurotus sp. on metabolic parameters and the immune response of
immunocompetent mice of both sexes.
Methods: The administration of the nutraceutical biopreparation was carried out for
14 days orally (1000 mg / kg) and serum hematological and biochemical markers
were subsequently determined and the innate and adaptive immune response was
evaluated by counting phagocytic cells and delayed hypersensitivity response,
respectively.
Results: The biopreparation increased the total leukocyte count, with a polarized
response that showed significant differences in lymphocyte and polymorphonuclear
populations in male and female mice, respectively. We also observed modulating
effects at the splenic cellular level and an increase in the number of peritoneal
macrophages in the treated females. Also, although no significant results were
detected in the levels of hemoglobin and total serum proteins, a differential behavior
was observed that favors the synthesis of the globulin fraction in the females that
received the nutraceutical. The supplement also stimulated the cellular immune
response in vivo in male mice.
Conclusions: The present study provides new evidence of the nutraceutical and
immunomodulatory effect of a Pleurotus sp. in immunocompetent mice of both sexes.
This suggests the estimation of immunomodulation markers both in preclinical studies
and in nutritional interventions in humans and also consider the influence of sex in
these studies.
REFERENCES
Prakash A, Saha H, Sumeetha V. Nutraceuticals: a functional food as the anti-aging drug and neuroprotective agent. Res J of Pharm, Bio and Chem Sci. 2014;5:936-43.
Kuppusamy P, Yusoff MM, Maniam GP, Ichwan SJ, Soundharrajan I, Govindan N, et al. Nutraceuticals as potential therapeutic agents for colon cancer: a review. Acta Pharm Sin B. 2014;4(3):173-81.
Elsayed A, El Enshasy H, Wadaan M, Aziz R. Mushrooms: A potential natural source of anti-Inflammatory compounds for medical applications. Mediators of Inflammation. 2014;Article ID 805841.
Valverde ME, Hernández-Pérez T, Paredes-López O. Edible Mushrooms: Improving human health and promoting quality life. Int J of Microbiol. 2015;Article ID 376387.
Smiderle FR, Baggio CH, Borato DG, Santana-Filho AP, Sassaki GL, Iacomini M, et al. Anti-inflammatory properties of the medicinal mushroom Cordyceps militaris might be related to its linear (1→3)-β-D-glucan. Plos ONE. 2014;9(10):e110266.
Baskaran A, Chua KH, Sabaratnam V, Ravishankar M, Kuppusamy UR. Pleurotus giganteus (Berk. Karun & Hyde), the giant oyster mushroom inhibits NO production in LPS/H2O2 stimulated RAW 264.7 cells via STAT 3 and COX-2 pathways. BMC Complementary and Alter Med. 2017;17:40.
Öztürk M, Tel G, Aydoğmuş F, Öztürk M, Duru E. The cooking effect on two edible mushrooms in Anatolia: fatty acid composition, total bioactive compounds, antioxidant and anticholinesterase activities. Records of Nat Prod. 2014;8(2):189-94.
Wasser SP. Medicinal mushroom science: Current perspectives, advances, evidences, and challenges. Biomedical J. 2014;37(6):345-56.
Deepalakshmi K, Mirunalini S. Pleurotus ostreatus: an oyster mushroom with nutritional and medicinal properties. J of Biochem Technol. 2014;5(2):718-26.
Gregori A, Švagel M, Pohleven J. Cultivation techniques and medicinal properties of Pleurotus spp. Food of Technol Biotechnol. 2007;45:238-49.
Shamtsyan MM, Konusova VG, Goloshchev AM, Maksimova YO, Panchenko AV, Petrishchev NN, et al. Immunomodulating and anti-tumor effects of basidiomycetes Pleurotus ostreatus (Jacq.: fr) P. Kumm and Pleurotus conucopiae (Pau. Ex Pers.) Rollan. J of Bio and Physiological Chem. 2004;4(3):157-61.
Wong JH, Ng TB, Cheung RCF, Ye XJ, Wang HX, Lam SK, et al. Proteins with antifungal properties and other medicinal applications from plants and mushrooms. Applied Microbiol Biotechnol. 2010;87(4):1221-35.
Scotland R, Stables MJ, Madalli S, Watson P, Gilroy DW. Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice. Blood. 2011;118 (22):5918-27.
Giefing-Krӧll C, Berger P, Lepperdinger G, Grubeck-Loebenstein B. How sex and age affect immune responses, susceptibility to infections, and response to vaccination. Aging Cell. 2015;14:309-21
Mc Murray JR. Plasma Proteins. In: A.H. Gowenbock, Eds. Practical Clinical Biochemistry. Heinemann, Oxford; 1988. p. 401-435.
Kim YSW, Maslinski XX, Zheng AC, Stevens XC, Li GH, Tesch VR, et al. Targeting the IL-15 receptor with an antagonist IL-15 mutant/Fcγ 2a protein blocks delayed type hypersensitivity. J of Immunol. 1998;160:5742-8.
Charles Rivers Inc. BALB/c mice hematology. North American Colonies. 2012 [citado 26 de octubre de 2012]. Disponible en: https://www.criver.com/sites/default/files/.../BALBcMouseClinicalPathologyData.pdf
Tolera KD, Abera S. Nutritional quality of Oyster Mushroom ( Pleurotus Ostreatus) as affected by osmotic pretreatments and drying methods. Food Sci Nut. 2017;5(5):989-96.
Rathore H, Prasad S, Sharma S. Mushroom nutraceuticals for improved nutrition and better human health: A review. PharmaNutrition. 2017;5 35-46.
Shankar AH. Modulación nutricional de la función inmunitaria y de la enfermedad. Infecciosa. En: Bowman y Russell A, editores. Conocimientos actuales sobre nutrición. 8a ed. Washington, DC: Organización Panamericana de la Salud; 2003. p. 746-61.
Ruggieri A, Anticoli S, D'Ambrosio A, Giordani L, Viora M. The influence of sex and gender on immunity, infection and vaccination. Ann Ist Super Sanità. 2016;52(2):198-204.
Tothova C, Nagy O, Kovac G. Serum proteins and their diagnostic utility in veterinary medicine: a review. Veterinarni Medicina. 2016;61:(9):475-96.
Tanaka A, Nishimura M, Sato Y, Sato H, Nishihira J. Enhancement of the Th1- phenotype immune system by the intake of Oyster mushroom (Tamogitake) extract in a double-blind, placebocontrolled study. J of Traditional and Complementary Med. 2016;6:424-30.
Zhang Z, Pereira S, Luo M, Matheson EM. Evaluation of blood biomarkers associated with risk of Malnutrition in older adults: A systematic review and metaanalysis. Nutrients. 2017;9(8):829
Verthelyi D. Sex hormones as immunomodulators in health and disease. Int Immunopharmacol. 2001;1:983-93.
Vitak T, Yurkiv B, Wasser S, Nevo E, SybirnaN. Effect of medicinal mushrooms on blood cells under conditions of diabetes mellitus. World J Diabetes. 2017;8(5):187- 201.
Yu S, Weaver V, Martin K, Cantorna MT. The effects of whole mushrooms during inflammation. BMC Immunol. 2009;20:10-2.
Kyakulaga AH, Ogwang PE, Obua C, Nakabonge G, Mwavu E. Immunomodulatory effects of aqueous extracts of Auricularia sp. and Pleurotus sp. mushrooms in cyclophosphamide immunosuppressed Wistar Rats. British J of Pharm Res. 2013;3(4):662-70.
Paterson RM, Lima N. Biomedical effects of mushrooms with emphasis on pure compounds. Biomedical J. 2014;37(6):357-68.
Cesta M. Normal Structure, Function, and Histology of the Spleen. Tox Pathology. 2006;34:455-65.
Bronte V, Pittet MJ. The spleen in local and systemic regulation of immunity. Immunity. 2013;14, 39(5):806-18.
Bouike G, Nishitani Y, Shiomi H, Yoshida M, Azuma T, Hashimoto T, et al. Oral treatment with extract of Agaricus blazei Murill enhanced Th1 response through intestinal epithelial cells and suppressed OVA-sensitized allergy in mice. Evidence- Based Complementary Alt Med. 2011; Article ID 532180.
Ni WY, Wu MF, Lio NCH, Yeh MY, Lu HF, Hsueh SH, et al. Extract of medicinal mushroom Agaricus blazei Murill enhances the non-specific and adaptive immune activities in BALB/c mice. In vivo. 2013;27:779-86.
Sun H, Zhang J, Chen F, Chen X, Wang H, Zhou Z, et al. Activation of RAW 264.7 macrophages by the polysaccharide from the roots of Actinidia eriantha and its molecular mechanisms. Carbohydrate Polymers. 2015;121:388-402.
Chen J, Seviour R. Medicinal importance of fungal β-(1→3), (1→6) glucans. Mycol Res. 2007;111:635-52.
Cutolo M, Sulli A, Capellino S, Villaggio B, Montagna P, Seriolo B, et al. Sex hormones influence on the immune system: basic and clinical aspects in autoimmunity. Lupus. 2004;13(9):635-8.
De León MA, Morales J. Dimorfismo sexual inmunitario: ¿Pueden los esteroides sexuales polarizar el perfil de citocinas Th1/Th2? Rev Invest Clín. 2006;58(2):161-9.
Ghazanfari T, Roya Y, Zohre F, Batool R. Macrophages activation and nitric oxide alterations in mice treated with Pleurotus florida. Immunopharmacol and Immunotoxicol 2010;32(1):47-50.
Limaye S. Tests for cell-mediated immunity. Australian Prescriber. 2010;33:3.
Abbas AK, Lichtman A. Pillai S. Cellular and Molecular Immunology. 9 th ed. Elsevier; 2017.
Paulik S, Svrcec K, Mojzisova J, Durove A, Benisek Z, Huska M, et al. The immunomodulatory effect of the soluble fungal glucan ( Pleurotus ostreatus) on delayed-hypersensitivity and phagocytic ability of blood leukocytes in mice. J of Med Veterinary Bio. 1996;43:129-35.
Bhatia A, Sekhon H, Kaur G. Sex Hormones and Immune Dimorphism. The Sci World J. 2014;ID 159150.
Karpuzoglu E, Phillips RA, Gogal RM Jr, Ahmed S. IFN-gamma-inducing transcription factor, T-bet is upregulated by estrogen in murine splenocytes: role of IL-27 but not IL-12. Mol Immunol. 2007;44(7):1808-14.
Angele M, Pratschke S, Hubbard WJ, Chaudry IH. Gender differences in sepsis Cardiovascular and immunological aspects. Virulence. 2014;5(1):12-9.
Fischer J, Jung N, Robinson R, Lehmann C. Sex differences in immune responses to infectious diseases. Infection. 2015;43(4):399-403.