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ISSN 1561-3054 (Electronic)

2018, Number 3

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Rev Cubana Med Trop 2018; 70 (3)

Introduction of new assays of real time polymerase's chain reaction for the diagnosis and surveillance of respiratory viruses

Martínez MMK, Piñón RA, Acosta HB, Valdés RO, Muné JM, Savón VC, Arencibia GA, González BG, González MG, Oropesa FS, Goyenechea HÁ, Hernández FB, Roque OR, Martínez AJ

Language: Spanish
References: 11
Page:
PDF size: 171.94 Kb.


ABSTRACT

Acute respiratory infections are the major cause of mortality and morbidity worldwide. Respiratory viruses are the main causative agents of acute respiratory infections. Rapid and accurate detection of these pathogens is critical for the treatment and prevention of the diseases these viral agents can cause. Currently, molecular diagnostic methods are useful tools for the virological detection of respiratory viruses due to its high sensitivity, specificity and their speed in obtaining results. The objective of this study was to introduce four multiplex real-time TR-RCP assays for the diagnosis and surveillance of fifteen virus causing acute respiratory infections. 2 441 clinical respiratory samples were processed in the period between September 2013 and April 2014 in the National Laboratory of Reference for Influenza Virus and other Respiratory Viruses. There were analyzed 2 352 nasopharyngeal exudates, 77 bronchial aspirations and 12 necropsy samples. Multiplex real-time TR-RCP was performed using TaqMan primers and probes previously published. From the 2 441 clinical samples studied, 1 290 were positive for some of the respiratory viruses, which represent 52.85 %. Syncytial respiratory virus was the most frequently detected virus (47.83 %), then influenza viruses (19 %) and human rhinovirus (14.73 %). The introduction at the National Reference Laboratory of the four multiplex real-time TR-RCP assays allows updating the algorithm for the diagnosis and surveillance of respiratory viruses in Cuba, as a contribution to the National Program for the Prevention and Control of Acute Respiratory Infection.


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