2019, Number S1
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ABSTRACTTo obtain a successful fertilization through Assisted Reproduction Techniques (ART), it is necessary to implement in vitro sperm capacitation and selection, with the purpose of obtaining a sample free of pollutants and decapacitation factors, as well as, obtaining the greatest amount of viable, mobile and capable spermatozoa to fertilize the oocyte. It is essential to perform the best sperm preparation technique depending on the type of ART, since the success of the treatment will depend largely on these, in this article we will discuss the procedure of various techniques (washing, conventional swim-up, density gradients, microfluid, MACS and Z potential) and the cases in which it is recommended to use each one of them.
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