González MA, García MG, Rangel VS, Pérez GD

Language: Spanish
References: 13
Page: 104-115
PDF size: 237.85 Kb.

ABSTRACT

Background: the exploration of training needs is essential for the design of educational strategies that increase the qualitative training of professionals, the quality of services and the rational use of resources.
Objective: to identify training needs for the genetic diagnosis of spondyloarthropathies and uveitis in physicians of the “Hermanos Ameijeiras” Clinical Surgical Teaching Hospital.
Methods: a descriptive quantitative-qualitative research was carried out, in which theoretical methods were used: analysis-synthesis, induction-deduction and historical-logical; empirical ones: documentary review of medical indications and a questionnaire to classify the training needs in manifest and hidden or covert, and felt and not felt about the correct indication of the genetic study for the diagnosis of spondyloarthropathies and uveitis; and mathematical-statistics for data- processing.
Results: the minority of the medical indications showed positive results; that is, the patients presented the HLA B-27U allele. The greatest knowledge shortcomings on the subject were shown by the residents, unlike the specialists who presented less wrong answers, although the latter expressed a greater perception of training needs than the residents, so they assumed a more open and flexible attitude towards overcoming.
Conclusions: training needs on the subject were identified, which allows generating strategies to achieve the rational use of molecular tools in the genetic diagnosis of spondyloarthropathies and uveitis in medical personnel.

REFERENCES

1. Seipp MT, Erali M, Wies RL, Wittwer C. HLA-B27 typing: evaluation of an allele-specific PCR melting assay and two flow cytometric antigen assays. Cytometry Part B (Clinical Cytometry).2005;63B:10-5.

2. Lin H, Gong YZ. Association of HLA-B27 with ankylosing spondylitis and clinical features of the HLA-B27-associated ankylosing spondylitis: a meta-analysis. Rheumatol Int. 2017;37(8):1267–80.

3. Chen B, Li J, He C, Li D, Tong W, Zou Y, et al. Role of HLA-B27 in the pathogenesis of ankylosing spondylitis. Mol Med Rep. 2017;15(4):1943-51.

4. De Keyser F. Spondyloarthropathies [editorial]. Curr Opin Rheumatol. 2006;18:329-31.

5. Reveille JD. The genetic basis of ankylosing spondylitis. Curr Opin Rheumatol. 2006;18:332-41.

6. Rangel S, González G, Monzón ME, Ortega A, García G. Frecuencia del HLA-B*27: algunas consideraciones sobre su uso como herramienta diagnóstica. Rev Cubana de Hematología, Inmunología y Hemoterapia [Internet]. 2017 [citado 22/12/2018];33(4):[aprox. 11 p.]. Disponible en: http://www.revhematologia.sld.cu/index.php/hih/article/view/625/538

7. Salas Perea R. La identificación de necesidades de aprendizaje. Educ Med Super [Internet]. 2003 [citado 22/12/2018];17(1):[aprox. 15 p.]. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S086421412003000100003&lng=es

8. Paramarta JE, Baeten D. Spondyloarthritis: from unifying concepts to improved treatment. Rheumatology. 2014;53(9):1547-59.

9. Akgul O, Ozgocmen S. Classification criteria for spondyloarthropathies. World J Orthop. 2011;2(12):107-15.

10. Tamay de Dios L, Ibarra C, Velasquillo C. Fundamentos de la reacción en cadena de la polimerasa (PCR) y de la PCR en tiempo real. Investigación en discapacidad. 2013;2(2):70-8.

11. Hui Y, Ming L, Kuang H, Hsien H, Su L, Hsien H, e tal. Sulfasalazine Treatment Suppresses the Formation of HLA-B27 Heavy Chain Homodimer in Patients with Ankylosing Spondylitis. Int J Mol Sci [Internet]. 2016[citado 08/01/2019];17(1):[aprox. 2 p.]. Disponible en: http://www.mdpi.com/1422-0067/17/1/46/htm

12. Servet A, Yusuf Z, Ismail S, Elif P, Esra G, Mehmet N. Do major histocompatibility complex tag single nucleotide polymorphisms accurately identify HLA-B27 in the Turkish population? International Journal of Rheumatic Diseases. 2017;20:2035–9.

13. Pérez-Acosta ME. Procedimiento para detectar necesidades de capacitación. Una propuesta [tesis]. UNAM. ENEP. Iztacala. [Internet]. 2005. [citado 08/01/2019]. Disponible en: http://132.248.9.34/pd2005/0601784/0601784.pdf