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2019, Number 1

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AbanicoVet 2019; 9 (1)

Use of plant extracts in vitro ruminal fermentation

Delgadillo-Ruiz L, Bañuelos-Valenzuela R, Echavarría-Cháirez F, Gallegos-Flores PI, Meza-López C
Full text How to cite this article

Language: Spanish
References: 33
Page: 1-17
PDF size: 1063.38 Kb.


Key words:

Plant extracts, Volatile fatty acids and Methane.

ABSTRACT

The objective was to obtain and characterize plant extracts of Larrea tridentata, Origanum vulgare, Artemisa ludoviciana and Ruta graveolens to be used in ruminal digestibility in vitro and to quantify the production of gas, volatile fatty acids and of methane. The chemical composition of the extracts was determinate by gas chromatography. Ruminal fluid was estimated according to Theodorou methodology for in vitro gas production. The determination of volatile fatty acids was carried out by gas chromatography. Methane was inferred from the concentration of volatile fatty acids. For the chemical composition, the following compounds was identified in the extracts: terpinene, limonene, linalool, thymol and carvacrol. In the digestibility that less gas produced at a volume of 100 µL of extract was L. tridentata and methane is reported 1.4 times more than the standard of alfalfa (0.514 mL g-1). For the concentration of 330 µL of the extract O. vulgaris was present inhibition of methane with respect to the control of alfalfa. This concluded that the extract four of O. vulgaris was the one that presented a better digestibility with respect to gas production, AGV and lower concentration of methane with respect to the control.


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AbanicoVet. 2019;9