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2019, Number 5-6

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Rev Sanid Milit Mex 2019; 73 (5-6)

Microorganisms isolated in the Neonatal Intensive Care Unit (NICU) of the Military Hospital for Women’s Specialties and Neonatology

Cisneros CSN, Romero FDA, Cacho CI,  Vargas OR

Language: Spanish
References: 15
Page: 269-276
PDF size: 265.42 Kb.


ABSTRACT

Introduction: The neonatal intensive care unit is one of the main services that provide necessary and vital care in newborns, these patients have a fragile immune system and it is necessary to maintain strict sanitary control on contact surfaces, in order to avoid the presence of nosocomial infections. Objective: To determine the frequency of bacteria and fungi on inert surfaces of the NICU. Material and methods: Monthly microbiological isolations were made on inert surfaces: beds, nurseries, and oxygen taps in different agars for their growth and identification by biochemical tests. Results: A prevalence of Gram-positive bacteria was found of 41.66% (1st sampling), followed by 75.6% (2nd sampling), 69.23% (3rd sampling) and 74.35% (4th sampling), with greater presence in nursery, Gram-negative bacteria showed a presence of 41.66% for the first sampling, followed by 17.07%, 17.3% and 12.82% respectively, mainly in handwash, the fungi remained below 16.68%. Gram-positive microorganisms prevailed S. epidermidis (48.73%), followed by S. saprophyticus (10.75%), Gram-negative was Acinetobacter baumanii (10.12%), Klebsiella sp. (2.53%) and Pseudomonas sp. (1.26%), fungi isolated were Candida sp. (9.49%). Conclusions: Gram-positive bacteria are found most frequently in cribs and S. epidermidis prevailing.


REFERENCES

  1. Novoa PJM, Milad AM, Vivanco GG, Fabres BJ, Ramírez FR. Recomendaciones de organización, características y funcionamiento en Servicios o Unidades de Neonatología. Vol. 80, Revista Chilena de Pediatría. 2009. p. 168-187.

  2. Adams CE, Smith J, Watson V, Robertson C, Dancer SJ. Examining the association between surface bioburden and frequently touched sites in intensive care. J Hosp Infect. 2017; 95 (1): 76-80.

  3. Haque M, Sartelli M, McKimm J, Bakar MA. Health care-associated infections-An overview. Vol. 11, Infection and Drug Resistance. 2018. pp. 2321-2333.

  4. Hayden MK, Blom DW, Lyle EA, Moore CG, Weinstein RA. Risk of hand or glove contamination after contact with patients colonized with vancomycin-resistant enterococcus or the colonized patients’ environment. Infect Control Hosp Epidemiol. 2007; 29 (02): 149-154.

  5. Russotto V, Cortegiani A, Raineri SM, Giarratano A. Bacterial contamination of inanimate surfaces and equipment in the intensive care unit. Vol. 3, Journal of Intensive Care. 2015. p. 54.

  6. Russotto V, Cortegiani A, Fasciana T, Iozzo P, Raineri SM, Gregoretti C et al. What Healthcare Workers Should Know about Environmental Bacterial Contamination in the Intensive Care Unit. Biomed Res Int. 2017; 2017: 1-7.

  7. Koneman E, Allen S, Janda W, Schreckenberger P, Winn W. Diagnóstico microbiológico. In: Diagnóstico Microbiológico 5a Edição. 2001.

  8. Caltzontzin Silva MP. Patógenos nosocomiales en superficies vivas e inertes en instituciones de salud del estado de Querétaro. Rev Context Saúde. 2015; 15 (28): 3-9.

  9. Arias-Flores R, Rosado-Quiab U, Vargas-Valerio A, Grajales-Muñiz C. Los microorganismos causantes de infecciones nosocomiales en el Instituto Mexicano del Seguro Social. Rev Med Inst Mex Seguro Soc [Internet]. 2016; 54 (1): 20-24. Available from: http://www.medigraphic.com/pdfs/imss/im-2016/im161d.pdf.

  10. Vargas Olmos R, Salgado Camarillo J. Agentes causales de neumonía nosocomial y su relación con microorganismos del ambiente hospitalario en pacientes de la unidad de cuidados intensivos. Enfermedades Infecc y Microbiol. 2004; 24 (2):

  11. Gonçalves CL, Mota F V., Ferreira GF, Mendes JF, Pereira EC, Freitas CH, et al. Airborne fungi in an intensive care unit. Braz J Biol. 2017; 78 (2): 265-270.

  12. Tolo I, Thomas JC, Fischer RSB, Brown EL, Gray BM, Robinson DA. Do Staphylococcus epidermidis Genetic Clusters Predict Isolation Sources? J Clin Microbiol. 2016; 54 (7): 1711-1719.

  13. Pinheiro L, Brito CI, Oliveira Ad, Pereira VC, Cunha Mde L. Staphylococcus epidermidis and Staphylococcus haemolyticus: detection of biofilm genes and biofilm formation in blood culture isolates from patients in a Brazilian teaching hospital. Diagn Microbiol Infect Dis. 2016; 86 (1): 11-14.

  14. Lee DC, Kananurak A, Tran MT, Connolly PA, Polage CR, Iwase T et al. Bacterial colonization of the hospitalized newborn: competition between Staphylococcus aureus and Staphylococcus epidermidis. Pediatr Infect Dis J [Internet]. 2019; 38 (7): 682-686. Available from: https://insights.ovid.com/crossref?an=00006454-900000000-96439.

  15. Erfani Y, Rasti A, Janani L. Prevalence of Gram-negative bacteria in ventilator-associated pneumonia in neonatal intensive care units: a systematic review and meta-analysis protocol. BMJ Open. 2016; 6 (10): e012298.




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