medigraphic.com
SPANISH

Acta Pediátrica de México

Órgano Oficial del Instituto Nacional de Pediatría
  • Contents
  • View Archive
  • Information
    • General Information        
    • Directory
  • Publish
    • Instructions for authors        
  • medigraphic.com
    • Home
    • Journals index            
    • Register / Login
  • Mi perfil

2020, Number S1

<< Back Next >>

Acta Pediatr Mex 2020; 41 (S1)

Interpretation of diagnostic tests for the SARS-Cov-2 virus

Díaz-Jiménez IV
Full text How to cite this article

Language: Spanish
References: 14
Page: 51-57
PDF size: 364.86 Kb.


Key words:

SARS-Cov-2, Pneumonia, COVID-19, RT-PCR, Antibodies, Infection, ELISA, Immunochromatography.

ABSTRACT

With a new viral infectious agent causing severe pneumonia identified in Wuhan as the SARS-Cov-2, the race against time for fast and safe diagnostic methods became a priority. Currently, for the diagnosis of Covid-19 disease in the acute phase, the RTPCR technique in the first week and to know the formation of antibodies to infection after the first week, serology is available with two techniques. ELISA recommended for clinical use in hospitals and Immunochromatography, more studies are missing to recommend its use.


REFERENCES

  1. Gorbalenya AE, et.al The species severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2. Nat Microbiol. 2020; 5: 536-44. https://doi.org/10.1038/s41564-020-0695-z.

  2. Padhi A, et al. Laboratory Diagnosis of Novel Coronavirus Disease 2019. En: Shailendra K. Coronavirus Disease 2019. Epidemiology, Pathogenesis, diagnosis and Therapeutics. 5 th ed. Singapore: Springer, 2020; 95-107. https://doi. org/10.1007/978-981-15-4814-7

  3. Secretaría de Salud. Lineamiento estandarizado para la vigilancia epidemiológica y por laboratorio de COVID-19. Secretaría de Salud México. https://www.gob.mx/cms/ uploads/attachment/file/552972/Lineamiento_VE_y_Lab_ Enf_Viral_20.05.20.pdf

  4. Cheng MP, et al. Diagnostic testing for severe acute respiratory syndrome-related Coronavirus-2: A narrative review. Ann Intern Med 2020; M20-1301. doi:10.7326/ M20-1301.

  5. CDC/NIH Biosafety in Microbiological and Biomedical Laboratories. https://www.cdc.gov/labs/pdf/CDCBiosafetyMicrobiologicalBiomedicalLaboratories- 2009-P.PDF

  6. CLSI Document M29 Protection of Laboratory Workers from Occupationally Acquired Infections. https://clsi.org/ standards/products/microbiology/documents/m29

  7. Centers for Disease Control. Refer to Interim Laboratory Safety Guidelines for Handling and Processing Specimens Associated with SARS-CoV-2. https://www.cdc.gov/ coronavirus/2019-ncov/lab/lab-biosafety-guidelines.html

  8. Manual de Instrucciones de uso Xpert Xpress SARS-CoV-2. https://www.cepheid.com/Package%20Insert%20Files/ Xpress-SARS-CoV-2/Xpert%20Xpress%20SARS-CoV-2%20 Assay%20SPANISH%20Package%20Insert%20302-3787- ES%20Rev.%20A.pdf

  9. Biofire RP. Manual de instrucción del usuario. https://docs. biofiredx.com/wp-content/uploads/BFR0000-8303-BioFire- RP2.1-Panel-Instructions-for-Use-EUA-EN.pdf

  10. Federal and Drug Administration. Coronavirus (COVID-19). Update: FDA Informs Public about possible accuracy concerns with abbot id now point of care Test. May 14, 2020. www.fda.gov.

  11. Ai T, et al. Correlation of chest CT and RT-PCR testing in coronavirus disease 2019 (COVID-19) in China: A report of 1014 cases. Radiology 2020; 200642. doi:10.1148/ radiol.2020200642.

  12. Sociedad Española de Pediatría. Recomendaciones de la Sociedad Española de Enfermedades infecciosas y Microbiología Clínica sobre el uso de las pruebas de detección de anticuerpos. https://seimc.org.

  13. Juanjuan Zhao, et al Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019. Clinical Infectious Diseases https://doi.org/10.1093/cid/ciaa344

  14. Nuccetelli M, et al. SARS-CoV-2 infection serology: a useful tool to overcome lockdown? Cell Death Discov 2020; 6: 38. https://doi.org/10.1038/s41420-020-0275-2.




2020     |     www.medigraphic.com

Mi perfil

C?MO CITAR (Vancouver)

Acta Pediatr Mex. 2020;41