2020, Number 2
Rev Mex Med Forense 2020; 5 (2)
Expression of mRNA of the HIF1-α gene in the frontal cortex of a murine model exposed to CO2 in lethal concentrations
Delgado-Carvajal A, Barrientos-Salcedo C
PDF size: 445.35 Kb.
ABSTRACTCarbon Monoxide (CO) is a colorless, odorless and tasteless gas produced by the incomplete combustion of hydrocarbons; it is toxic due to its high affinity for hemoglobin producing a state of hypoxia leading to death. In most cases of death due to CO poisoning, there are no pathognomonic lesions; however, in some cases the findings at autopsy are indicative of the causative agent; confirmation requires detection of carboxyhemoglobin, test of contradictory results. In the present work, the expression of the mRNA of the HIF1-α gene was analyzed by RT-PCR, as a marker to identify CO intoxication from brain tissue of rats exposed to CO at concentrations of 40% CO2 / 60% O2 and 60 % CO2 / 40% O2. The diagnosis of death by carbon monoxide poisoning is only based on the clinical characteristics presented by the individual; on the other hand, there is no toxicological protocol established in the country to identify CO intoxication. Our results indicated no difference in the expression of HIF1-α between the exposed animals and the control group; it may be due to the sensitivity of the test, so it is proposed that RT-PCR is used in real time.