Martínez-Benavidez E, Herrera-Rodríguez SE, Lugo-Melchor OY, Hernández-Martínez J, Higuera-Ciapara I

Language: Spanish
References: 26
Page: 96-102
PDF size: 328.48 Kb.

ABSTRACT

Nanoencapsulation using polymers such as chitosan is a biotechnological alternative for the targeted and efficiency administration of molecules with low bioavailability, but with important biological activity such as capsaicin. Capsaicin has been reported to induce apoptosis in several cancer cells, including glioblastoma multiforme cells. However, the use of capsaicin is limited due to its poor aqueous solubility and low bioavailability, so the development of new approaches such as the use of nanostructured systems for the release of active compounds with these characteristics represents an alternative of enormous potential for the inhibition of cancer cells. Objetive: Evaluate antitumor effect of nanocapasules of chitosan with capsaicin on glioblastoma cells. Methods: Evaluation of antitumor activity using the MTT method against two glioblastoma cell lines of two encapsulation nanosystems (nanoemulsions and nanocapsules). Results: Capsaicin loaded nanocapsules and nanoemulsions did significantly reduced cell viability of glioblastoma lines H4 and U118MG compared to their unloaded counterparts. In addition, it is observed that free capsaicin had major effect on cell viability compared with temozolomide. Conclusions: Design and development of nanostructured chitosan systems is of great interest due to its potential applications as an alternative for the supply of capsaicin and to avoid the adverse properties of the administration of free capsaicin.

REFERENCES

1. Agrahari V. 2018. Novel drug delivery systems, devices, and fabrication methods. Drug Delivery and Translational Research 8:303-306

2. Xinyu R, Yinghua X, Xiaomei H, Tao C, Yingming W, Yuanyuan L. 2011. Applications of Polymeric Nanocapsules in Field of Drug Delivery Systems. Current Drug Discovery Technologies 8:173- 187.

3. Mora-Huertas CE, Fessi H, Elaissari A. 2010. Polymer-based nanocapsules for drug delivery. International Journal of Pharmaceutics 385:113-142.

4. Goycoolea FM, Valle-Gallego A, Stefani R, Menchicchi B, David L, Rochas C, Santander-Ortega MJ, Alonso MJ. 2012. Chitosan-based nanocapsules: physical characterization, stability in biological media and capsaicin encapsulation. Colloid and Polymer Science 290:1423-1434.

5. Kaiser M, Pereira S, Pohl L, Ketelhut S, Kemper B, Gorzelanny C, Galla HJ, Moerschbacher BM, Goycoolea FM. 2015. Chitosan encapsulation modulates the eff ect of capsaicin on the tight junctions of MDCK cells. Scientifi c Reports 5:10048.

6. Kleine-Brueggeney H, Zorzi GK, Fecker T, El Gueddari NE, Moerschbacher BM, Goycoolea FM. 2015. A rational approach towards the design of chitosan-based nanoparticles obtained by ionotropic gelation. Colloids and Surfaces B-Biointerfaces 135:99-108.

7. Santos-Carballal B, Aaldering LJ, Ritzefeld M, Pereira S, Sewald N, Moerschbacher BM, Gotte M, Goycoolea FM. 2015. Physicochemical and biological characterization of chitosanmicroRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells. Scientifi c Reports 5.

8. Smith J, Wood E, Dornish M. 2004. Eff ect of chitosan on epithelial cell tight junctions. Pharmaceutical Research 21:43-49.

9. Ye YJ, Wang Y, Lou KY, Chen YZ, Chen RJ, Gao F. 2015. Th e preparation, characterization, and pharmacokinetic studies of chitosan nanoparticles loaded with paclitaxel/dimethyl-betacyclodextrin inclusion complexes. International Journal of Nanomedicine 10:4309-4319.

10. Pramanik KC, Boreddy SR, Srivastava SK. 2011. Role of Mitochondrial Electron Transport Chain Complexes in Capsaicin Mediated Oxidative Stress Leading to Apoptosis in Pancreatic Cancer Cells. Plos One 6.

11. Brown KC, Witte TR, Hardman WE, Luo H, Chen YC, Carpenter AB, Lau JK, Dasgupta P. 2010. Capsaicin displays anti-proliferative activity against human small cell lung cancer in cell culture and nude mice models via the E2F pathway. Plos One 5:e10243.

12. Mori A, Lehmann S, O’Kelly J, Kumagai T, Desmond JC, Pervan M, McBride WH, Kizaki M, Koeffl er HP. 2006. Capsaicin, a component of red peppers, inhibits the growth of androgenindependent, p53 mutant prostate cancer cells. Cancer Research 66:3222-3229.

13. Amantini C, Mosca M, Nabissi M, Lucciarini R, Caprodossi S, Arcella A, Giangaspero F, Santoni G. 2007. Capsaicin-induced apoptosis of glioma cells is mediated by TRPV1 vanilloid receptor and requires p38 MAPK activation. Journal of Neurochemistry 102:977-990.

14. Jeon JH, Choi YJ, Han IH, Choi BK, Cha SH, Cho WH. 2012. Capsaicin-induced apoptosis in the human glioblastoma U87MG cells via p-38 MAPK and Bcl-2/Bax signaling pathway. Molecular & Cellular Toxicology 8:69-76.

15. Kaiser M, Higuera I, Goycoolea FM. 2017. Capsaicinoids: Occurrence, Chemistry, Biosynthesis, and Biological Eff ects. In E. M. Yahia (Ed.). Fruit and Vegetable Phytochemicals: Chemistry and Human Health, Volume I, Second Ed. (499–513). John Wiley & Sons Ltd.

16. Glaser T, Han I, Wu LQ, Zeng X. 2017. Targeted Nanotechnology in Glioblastoma Multiforme. Frontiers in Pharmacology 8.

17. Suk K. 2012. Proteomic Analysis of Glioma Chemoresistance. Current Neuropharmacology 10:72-79.

18. Carmo A, Carvalheiro H, Crespo I, Nunes I, Lopes MC. 2011. Eff ect of temozolomide on the U-118 glioma cell line. Oncology Letters 2:1165-1170.

19. Yang L, Gao SY, Asghar S, Liu GH, Song J, Wang X, Ping QN, Zhang C, Xiao YY. 2015. Hyaluronic acid/chitosan nanoparticles for delivery of curcuminoid and its in vitro evaluation in glioma cells. International Journal of Biological Macromolecules 72:1391-1401.

20. Xu Y, Asghar S, Yang L, Li H, Wang Z, Ping Q, Xiao Y. 2017. Lactoferrin-coated polysaccharide nanoparticles based on chitosan hydrochloride/hyaluronic acid/PEG for treating brain glioma. Carbohydrate Polymers. 157(10):419-428.

21. Rao W, Wang H, Han J, Zhao S, Dumbleton J, Agarwal P, Zhang W, Zhao G, Yu J, Zynger DL, Lu X, He X. 2015. Chitosan- Decorated Doxorubicin-Encapsulated Nanoparticle Targets and Eliminates Tumor Reinitiating Cancer Stem-like Cells. CAN Nano 9(6):5725-5740.

22. Goertz O, Lauer H, Hirsch T, Daigeler A, Harati K, Stricker I, Lehnhardt M, von der Lohe L. 2016. Evaluation of angiogenesis, epithelialisation and microcirculation aft er application of polyhexanide, chitosan and sodium chloride in rodents. International Wound Journal 13:1161-1167.

23. Pourgholi F, Hajivalili M, Farhad JN, Kafi l HS, Yousefi M. 2016. Nanoparticles: Novel vehicles in treatment of Glioblastoma. Biomedicine & Pharmacotherapy 77:98-107.

24. Gil Y-G, Kang M-K. 2008. Capsaicin induces apoptosis and terminal diff erentiation in human glioma A172 cells. Life Sciences 82:997-1003.

25. Lee YS, Nam DH, Kim J-A. 2000. Induction of apoptosis by capsaicin in A172 human glioblastoma cells. Cancer Letters 161:121-130.

26. Liu Y-P, Dong F-X, Chai X, Zhu S, Zhang B-L, Gao D-S. 2016. Role of Autophagy in Capsaicin-Induced Apoptosis in U251 Glioma Cells. Cellular and Molecular Neurobiology 36:737-743.