Hernandez A, Fajardo-Sanchez AR, Cabrera RA, Ortiz N, Gonzalez-Aznar E, Ramirez-Bencomo F, Acevedo R, Menendez T

Language: English
References: 10
Page: 3201-3204
PDF size: 329.89 Kb.

ABSTRACT

One of the serologic criteria to diagnose Rheumatoid Arthritis (RA) is the determination of serum Rheumatoid factor (RF), which is an autoantibody targeting IgG. It can be detected by agglutination assays using commercially-available polystyrene latex-based reagents, which are marketed at relatively high cost. In low-income settings, homemade latex-based reagents can be a cost-effective alternative. Multilatex^® is a registered trademark of polystyrene latex. It is produced at the Center of Biomaterials of the University of Havana (Havana, Cuba) for the development of diagnostic reagents. This work was aimed to obtain a polystyrene latex-based homemade system for RF detection, using Multilatex^® and purified IgG as RF target. IgG was purified from commercially available purified human blood gamma globulin fraction, through affinity chromatography with protein G and adsorbed onto 800 nm-diameter Multilatex^® polystyrene spheres. The RF detection system was calibrated to a detection limit of 8 international units/mL of RF in patient serum, and evaluated in an agglutination test with 30 positive and 30 negative RF human sera. The developed system behaved similar when compared to a commercial reagent of 100 % specificity and 100 % sensitivity, indicating that it could be developed for diagnosis of RA.

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