Parra PLM,Acosta GE

Language: Spanish
References: 7
Page: 151-154
PDF size: 115.10 Kb.

ABSTRACT

The aim of this investigation was to compare several biological indicators (BI) against Bacillus subtilis 9372 y B. stearothermophilus 7953 strains from the ATCC catalog, according to tests described in the Mexican Pharmacopoea (MP).
It was observed that ATCC strains and BI from foreign manufacturers have the morphological, biochemical and growth characteristics described in the MP. Indeed, foreign manufacturers claim that their products are elaborated with ATCC strains.
In contrast, some BI made in Mexico are not elaborated with ATCC strains. A Mexican B. subtilis isolate intended for use in dry heat actually behaves like ATCC-9372 strain, whereas another Mexican isolate used for verification of steam sterilization (supposedly B. stearothermophilus) behaves as a non pigmenting B. subtilis.
BI manufacturers must document their claims truthfulness and provide information that will allow the end user to select adequate products. Instructions for use must be precise.

REFERENCES

1. Miller CH. Sterilization: disciplined microbial control. Dent Clin North Am 1991; 35: 335-339.

2. Métodos generales de análisis. En: Farmacopea de los Estados Unidos Mexicanos. Sexta Edición. Secretaría de Salud, México D.F. 1994: 165-172.

3. American Dental Association: Council on Dental Materials, Instruments and Equipment; Council on Dental Practice; and Council on Dental Therapeutics. Infection control recommendations for the dental office and the dental laboratory. JADA 1992; (suppl).

4. Centers for Disease Control and Prevention. Recommended infection-control practices for dentistry, 1993. MMWR 1993; 41(RR-8): 1-12.

5. Office Sterilization and Asepsis Procedures Research Foundation. Infection control in dentistry guidelines. Annapolis Maryland. 1995.

6. Modificación a la Norma Oficial Mexicana NOM-013-SSA2-1994 «Para La Prevención de Enfermedades Bucales», Secretaría de Salud. Diario Oficial de la Federación, Enero 21, 1999.

7. Norma Oficial Mexicana NOM-087-ECOL-1995 «Separación, Envasado, Almacenamiento, Recolección, Transporte, Tratamiento y Disposición Final de los Residuos Peligrosos Biológico-Infecciosos que se Generan en Establecimientos que presten Atención Médica». Secretaría del Medio Ambiente, Recursos Naturales y Pesca. Diario Oficial de la Federación, Noviembre 7, 1995.