Morales-Ubaldo A, Hernández-Alvarado J, Valladares-Carranza B, Velázquez-Ordoñez V, Delgadillo-Ruiz L, Rosenfeld- Miranda C, Rivero-Pérez N, Zaragoza-Bastida A

Language: Spanish
References: 26
Page: 1-10
PDF size: 681.51 Kb.

ABSTRACT

The World Organization for Animal Health (OIE) mentions that the inappropriate use of antibiotics has led to the emergence of strains of bacteria resistant to the mechanisms of action of these drugs, due to the scarcity of effective therapies, the development of new treatment options is required for diseases that affect health, in this respect plant extracts or their pure compounds offer an alternative. The aim of the present investigation was to characterize and evaluate the in vitro antibacterial activity of the hydroalcoholic extract of Croton draco on bacteria of sanitary importance. The extract was obtained by hydroalcoholic maceration, a qualitative and chemical characterization of the extract was carried out, and the antibacterial activity was determined by the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC). The qualitative characterization tests performed on the hydroalcoholic extract of Croton draco indicate the presence of phenolic compounds, terpenes, saponins and alkaloids, by gas chromatography the presence of thymol and carvacrol was determined in concentrations of 0.5340 mg/ml and 0.4206 mg/mL respectively. The bacteria showed different degrees of sensitivity to the hydroalcoholic extract, however, greater activity was determined against Gram positive bacteria such as Listeria monocytogenes, Staphylococcus aureus and Bacillus subtilis.

REFERENCES

1. BALOUIRI M, Sadiki M, Ibnsouda SK. 2016. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis. 6(2):71-79. https://doi.org/10.1016/j.jpha.2015.11.005

2. BAÑUELOS-VALENZUELA R, Delgadillo L, Chairez F, Delgadillo O, Meza-López C. 2018. Composición química y FTIR de extractos etanólicos de Larrea tridentata, Origanum vulgare, Artemisa ludoviciana y Ruta graveolens. Agrociencia. 52(3): 309-321. https://dialnet.unirioja.es/servlet/articulo?codigo=6423180.

3. BARRERO AF, Quílez del Moral JF, Lara A, Herrador MM. 2005. Antimicrobial Activity of Sesquiterpenes from the Essential Oil of Juniperus thurifera Wood. Planta Med. 71(01): 67-71. https://doi:10.1055/s-2005-837753

4. CABRERA CE, Gómez RF, Zúñiga AE. 2007. La resistencia de bacterias a antibióticos, antisépticos y desinfectantes una manifestación de los mecanismos de supervivencia y adaptación. Colombia Médica. 38 (2): 149-158. http://www.scielo.org.co/pdf/cm/v38n2/v38n2a07.pdf

5. CLSI (Clinical and Laboratory Standards Institute). 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard- Ninth Edition. USA. Pp. 88.

6. CUSHNIE TPT, Lamb AJ. 2005. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents. 26(5): 343-356. https://doi.org/10.1016/j.ijantimicag.2005.09.002

7. CUSHNIE TPT, Cushnie B, Lamb AJ. 2014. Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. International Journal of Antimicrobial Agents. 44(5):377-386. https://doi:https://doi.org/10.1016/j.ijantimicag.2014.06.001

8. DU E, Gan L, Li Z, Wang W, Liu D, Guo Y. 2015. In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens. Journal of animal science and biotechnology. 6:58-58. https://doi:10.1186/s40104-015-0055-7.

9. GONELIMALI FD, Lin J, Miao W, Xuan J, Charles F, Chen M, Hatab SR. 2018. Antimicrobial Properties and Mechanism of Action of Some Plant Extracts Against Food Pathogens and Spoilage Microorganisms. Frontiers in microbiology. 9: 1639-1639. https://doi:10.3389/fmicb.2018.01639

10. GUPTA D, Bleakley B, Gupta, RK. 2008. Dragon's blood: Botany, chemistry and therapeutic uses. Journal of Ethnopharmacology. 115(3):361-380. doi: https://doi.org/10.1016/j.jep.2007.10.018

11. HERNÁNDEZ-ALVARADO JL, Zaragoza-Bastida A, López-Rodríguez GM, Peláez-Acero A, Olmedo-Juárez A, Rivero-Perez N. 2018. Actividad antibacteriana y sobre nematodos gastrointestinales de metabolitos secundarios vegetales: enfoque en Medicina Veterinaria. Abanico veterinario. 8(1): 14-27. https://dx.doi.org/10.21929/abavet2018.81.1

12. KAEWPIBOON C, Lirdprapamongkol K, Srisomsap C, Winayanuwattikun P, Yongvanich, T, Puwaprisirisan, Puwaprisirisan P,Svasti J, Assavalapsakul W. 2012. Studies of the in vitro cytotoxic, antioxidant, lipase inhibitory and antimicrobial activities of selected Thai medicinal plants. BMC Complementary and Alternative Medicine. 12(1): 217. https://doi.org/10.1186/1472-6882-12-217.

13. LAI CC, Lee K, Xiao Y, Ahmad N, Veeraraghavan B, Thamlikitkul V, Hsueh PR. 2014. High burden of antimicrobial drug resistance in Asia. Journal of Global Antimicrobial Resistance. 2(3): 141-147. https://doi.org/10.1016/j.jgar.2014.02.007.

14. LAVOR AK LS, Matias EFF, Alves EF, Santos BS, Figueredo FG, Lima LF, Coutinho HDM. 2014. Association between drugs and herbal products: In vitro enhancement of the antibiotic activity by fractions from leaves of Croton campestris A. (Euphorbiaceae). European Journal of Integrative Medicine. 6(3):301-306. https://doi.org/10.1016/j.eujim.2014.03.002.

15. MANDAL P, Sinha BSP, Mandal NC. 2005. Antimicrobial activity of saponins from Acacia auriculiformis. Fitoterapia. 76(5): 462-465. https://doi.org/10.1016/j.fitote.2005.03.004.

16. MARTÍNEZ GJ, Jiménez - Escobar ND. 2017. Plantas de interés veterinario en la cultura campesina de la Sierra de Ancasti (Catamarca, Argentina). Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. 16(4):329-346. https://www.blacpma.usach.cl/sites/blacpma/files/articulo_1_-_1292_-_329_-_346_0.pdf

17. MAXIA A, Lancioni C, Mura L, Mongiano PD. 2005. Plantas usadas en la practica etnoveterinaria en el Nuorese (Cerdena, Italia). Revista de Fitoterapia. 5(2). 155-161. https://docplayer.es/6533436-Plantas-usadas-en-la-practica-etnoveterinaria-en-elnuorese- cerdena-italia.html

18. MOTHANA RA, Lindequist U, Gruenert R, Bednarski PJ. 2009. Studies of the in vitro anticancer, antimicrobial and antioxidant potentials of selected Yemeni medicinal plants from the island Soqotra. BMC complementary and alternative medicine. 9(1):7. https://doi.org/10.1186/1472-6882-9-7.

19. OIE (Organización Internacional de Salud Animal). 2016. Estrategia de la OIE sobre la resistencia a los agentes antimicrobianos y su uso prudente. www.oie.int/fileadmin/Home/esp/Media_Center/docs/pdf/PortalAMR/ES_OIEAMRstrategy. pdf

20. PERES, MTLP, Monache FD, Cruz AB, Pizzolatti MG, Yunes RA. 1997. Chemical composition and antimicrobial activity of Croton urucurana Baillon (Euphorbiaceae). Journal of Ethnopharmacology. 56(3):223-226. https://doi.org/10.1016/S0378- 8741(97)00039-1

21. RIVERO-PEREZ N, Ayala-Martínez M, Zepeda-Bastida A, Meneses-Mayo M, Ojeda- Ramírez D. 2016. Anti-inflammatory effect of aqueous extracts of spent Pleurotus ostreatus substrates in mouse ears treated with 12-O-tetradecanoylphorbol-13-acetate. Indian Journal of Pharmacology. 48(2):141-144. https://doi.org/10.4103/0253- 7613.178826.

22. SALATINO A, Salatino MLF, Negri G. 2007. Traditional uses, chemistry and pharmacology of Croton species (Euphorbiaceae). Journal of the Brazilian Chemical Society. 18:11-33. http://dx.doi.org/10.1590/S0103-50532007000100002

23. SELOWA SC, Shai LJ, Masoko P, Mokgotho MP, Magano SR. 2009. Antibacterial activity of extracts of three Croton species collected in Mpumalanga region in South Africa. African Journal of Traditional Complementary and Alternative Medicine. 7(2):98-103. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021162/pdf/AJTCAM0702-0098.pdf

24. SOUZAA SMD, Monacheb FD, Smania A. 2005. Antibacterial Activity of Coumarins. Naturforsch. 60: 693-700. https://pdfs.semanticscholar.org/d5a6/502543e2174bd3da1f1d6bb6d73b98784b4e.pdf

25. UPADHYAY A, Karumathil DP, Upadhyaya I, Bhattaram V, Venkitanarayanan K. 2016. Controlling Bacterial Antibiotic Resistance Using Plant-Derived Antimicrobials. En: Kateryna K, Mahendra R. Antibiotic Resistance. Academic Press. Pp. 205-226. ISBN 9780128036426. https://doi.org/10.1016/B978-0-12-803642-6.00010-1.

26. VALLE DL, Andrade JI, Puzon JJM, Cabrera EC, Rivera WL. 2015. Antibacterial activities of ethanol extracts of Philippine medicinal plants against multidrug-resistant bacteria. Asian Pacific Journal of Tropical Biomedicine. 5(7): 532-540. https://doi.org/10.1016/j.apjtb.2015.04.005