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2020, Number 1

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AbanicoVet 2020; 10 (1)

Comparison of three blood sampling sites, for physiometabolic evaluation piglets

Sánchez-Salcedo J, González-Lozano M, Bonilla-Jaime H, González-Hernández M, Ballesteros-Rodea G, Orozco-Gregorio H

Language: Spanish
References: 17
Page: 1-9
PDF size: 390.54 Kb.


ABSTRACT

Blood drawing of newborn piglets is necessary for physio-metabolic profiles assessment, and to determine gas change levels in it, what is used in various phenomena diagnosis, such as, intrapartum asphyxia a non-infectious condition that causes a high rate of neonatal mortality in pigs. This study aimed to test three of the most common blood sampling sites used in piglets: umbilical cord (UC), retroorbital sinus (ROS), and cava vein (VC), besides determining the differences among them, concerning to physio-metabolic variables and the acid-base balance. Thirty-two healthy non asphyxiated piglets were randomly selected, coming from nine eutocic farrowing to carry out the three sampling sites. All of the physio-metabolic concentrations determined showed basal values within normal fetal ranges corresponding to the sampling site. However, UC values were more suitable for gas exchange diagnosis in asphyxia due to their similarity with physiological parameters of a normal newborn (pCO2= 36.10 ± 2.03, pO2= 32.68 ± 3.03), whereas for the metabolic profile (glucose and lactate) ROS values were more accurate for measuring a common lactate and glycaemia profile than the other sites. Therefore, when a physio-metabolic profile is required, the methodological approach of the blood sampling site should be adequate to obtain the data needed for research following planned objectives and subsequent interpretation.


REFERENCES

  1. P, Som S, Choudhuri N, Das H. 2009. Contribution of the blood glucose level in perinatal asphyxia. European Journal of Pediatrics. 168:833–838. ISSN: 1432-1076. https://doi.org/10.1007/s00431-008-0844-5

  2. BAXTER EM, Edwards AS. 2018. Piglet mortality and morbidity. En: Advances in Pig Welfare. Elsevier. Pp. 73–100. ISBN: 9780081011195. https://doi.org/10.1016/B978- 0-08-101012-9.00003-4

  3. BAXTER EM, Jarvis S, Sherwood L, Farish M, Roehe R, Lawrence AB, Edwards SA. 2011. Genetic and environmental effects on piglet survival and maternal behaviour of the farrowing sow. Applied Animal Behaviour Science. 130:28-41. ISSN: 0168-1591. https://doi.org/10.1016/j.applanim.2010.11.020

  4. BOARDMAN JP, Hawdon JM. 2015. Hypoglycaemia and hypoxic-ischaemic encephalopathy. Developmental Medicine & Child Neurology. 57:29–33. ISSN: 1469- 8749. https://doi.org/10.1111/dmcn.12729

  5. HALL JE. 2016. Guyton y Hall. Compendio de Fisiología Médica. Elsevier Health Sciences Spain. Pp. 157-166. ISBN: 9788491139253.

  6. HERRERA-MARSCHITZ M, Neira-Pena T, Rojas-Mancilla E, Espina-Marchant P, Esmar D, Perez R, Muñoz V, Gutierrez-Hernandez M, Rivera B, Simola N, Bustamante D, Morales P, Gebicke-Haerter PJ. 2014. Perinatal asphyxia: CNS development and deficits with delayed onset. Frontiers in Neuroscience. 8:1–11. ISSN: 1662-453X. https://doi.org/10.3389/fnins.2014.00047

  7. MARTZ P, Georgiev P, Wehrend A. 2017. Prolonged second stage labour and consequences of hypoxia in the neonate: A review. Bulgarian Journal of Veterinary Medicine. 22:131–142. ISSN: 1313-3543. https://doi.org/10.15547/bjvm.2012

  8. OROZCO-GREGORIO H, Bonilla-Jaime H, Mota-Rojas D, Trujillo-Ortega ME, Roldan- Santiago P, Martínez-Rodríguez R, Borderas-Tordesillas F, Flores-Peinado S, Mora- Medina P, Ramírez-Necoechea R. 2012. Effects of subcutaneous administration of caffeine on the physiometabolic profile of low-birthweight neonate piglets. Animal Production Science. 52:981. ISSN: 1836-5787. https://doi.org/10.1071/AN11199

  9. OROZCO-GREGORIO H, Mota-Rojas D, Alonso-Spilsbury M, Olmos-Hernandez A, Ramirez-Necoechea R, Velazquez-Armenta EY, Nava-Ocampo AA, Hernandez- Gonzalez R, Trujillo-Ortega ME, Villanueva-Garcia D. 2008. Short-term neurophysiologic consequences of intrapartum asphyxia in piglets born by spontaneous parturition. International Journal of Neuroscience. 118:1299–1315. ISSN: 1563-5279. https://doi.org/10.1080/00207450701872846

  10. ROOTWELT V, Reksen O, Farstad W, Framstad T. 2013. Postpartum deaths: Piglet, placental, and umbilical characteristics. Journal of Animal Science. 91:2647–2656. ISSN: 1525-3163. https://doi.org/10.2527/jas.2012-5531

  11. SÁNCHEZ-SALCEDO J, Bonilla-Jaime H, González-Lozano M, Hernández-Arteaga S, Greenwell-Beare V, Vega-Manriquez X, González-Hernández M, Orozco-Gregorio H. 2019a. Therapeutics of neonatal asphyxia in production animals: a review. Veterinárni medicína. 64(05):191-203. ISSN: 1805-9392. https://doi.org/10.17221/86/2018-vetmed

  12. SÁNCHEZ-SALCEDO JA, Orozco-Gregorio H, González-Lozano M, Roldán-Santiago P, González-Hernández M, Ballesteros-Rodea G, Bonilla-Jaime H. 2019b. Caffeine administered to pregnant sows improves piglet vitality, gas exchange and body weight gain. Animal Reproduction Science. 208:1-8. ISSN: 0378-4320. https://doi.org/10.1016/j.anireprosci.2019.106120

  13. SEDDON YM, Cain PJ, Guy JH, Edwards SA. 2013. Development of a spreadsheet based financial model for pig producers considering high welfare farrowing systems. Livestock Science. 157:317-321. ISSN: 1871-1413. https://doi.org/10.1016/j.livsci.2013.07.003

  14. TRUJILLO-ORTEGA ME, Mota-Rojas D, Olmos-Hernández A, González M, Orozco H, Ramírez-Necoechea R, Nava-Ocampo AA. 2007. A study of piglets born by spontaneous parturition under uncontrolled conditions: could this be a naturalistic model for the study of intrapartum asphyxia? Acta Biomedica: Atenei Parmensis. 78(1): 29-35. ISSN: 2531-6745. https://doi.org/10.1080/00207450701872846

  15. VANDERHAEGHE C, Dewulf J, de Kruif A, Maes D. 2013. Non-infectious factors associated with stillbirth in pigs: A review. Animal Reproduction Science. 139:76-88. ISSN: 0378-4320. https://doi.org/10.1016/j.anireprosci.2019.106120

  16. VAN DIJK AJ, Van Rens BTTM, Van der Lende T, Taverne MAM. 2005. Factors affecting duration of the expulsive stage of parturition and piglet birth intervals in sows with uncomplicated, spontaneous farrowings. Theriogenology. 64:1573-1590. ISSN: 0093-691X. https://doi.org/10.1016/j.theriogenology.2005.03.017

  17. YLI BM, Kjellmer I. 2016. Pathophysiology of foetal oxygenation and cell damage during labour. Best Practice & Research: Clinical Obstetrics & Gynaecology. 30:9–21. ISSN: 1521-6934. https://doi.org/10.1016/j.bpobgyn.2015.05.004.




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