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>Journals >Gaceta Médica de México >Year 2015, Issue 2

López-Macay A, Ruiz-Medina EJ, Ventura-Gallegos JL, Arechavaleta-Velasco F, Gómez-Quiroz LE, Konigsberg-Fainstein M, Zentella-Dehesa A
Characterization of hemodynamic ex vivo model to study endothelial activation by TNF-α in prefunded human veins
Gac Med Mex 2015; 151 (2)

Language: Español
References: 38
Page: 206-215
PDF: 318.83 Kb.

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Inflammation is recognized as part of the etiology of numerous diseases. The interaction among cells of the immunological system with local cells and molecules, such as cytokines and chemokines, allows cellular activation and response amplification. The importance of several physicochemical factors like frictional force, vascular flow, shear stress, and pressure is now recognized because they are known to modulate genetic expression and endothelial activation; however, there are very few studies that recreate such cellular microenvironments. Hence, it is of paramount importance to develop new models that will mimic physiological conditions. Our aim was to improve a human vein ex vivo model that would allow endothelial activation in flow conditions, to study the molecular components during adhesion, taking into consideration physicochemical parameters such as flow and shear stress. Endothelial umbilical human vein was used and activated with TNF-α in order to determine U937 monocytic cells adhesion, as well as cytokines secretion and ICAM-1 expression. This model will allow leukocyte adhesion studies, using different inflammatory stimulus, along with the signaling pathways involved in several pathologies.

Key words: Endothelium, Adhesion, Inflammation, Vascular flow, Perfusion.


  1. Phillipson M, Heit B, Colarusso P, Liu L, Ballantyne CM, Kubes P. Intraluminal crawling of neutrophils to emigration sites: a molecularly distinct process from adhesion in the recruitment cascade. JEM. 2006;203(12):2569-75Z

  2. Tisato V, Zauli G, Voltan R, et al. Endothelial Cells Obtained from Patients Affected by Chronic Venous Disease Exhibit a Pro-Inflammatory Phenotype. PlosOne. 2012;7(6):e39543.

  3. Harris TA, Yamakuchi M, Ferlito M, Mendell JT, Lowenstein CJ. MicroRNA-126 regulates endothelial expression of vascular cell adhesion molecule 1. PNAS. 2008;105(5):1516-21.

  4. Luscinskas FW. Neutrophil CD44 rafts and rolls. Blood. 2010;116(3): 314-5.

  5. Dunne JL, Collins RG, Beaudet AL, Ballantyne CM, Ley K. Inflammation Leukocyte Rolling in TNF-a-Induced Adhesion Molecule-1 to Mediate Slow Mac-1, but Not LFA-1, Uses, J Immunol. 2003;171(11):6105-11.

  6. Fukuda S, Schmid-Schonbein GW. Regulation of CD18 expression on neutrophils in response to fluid shear stress. PNAS;100(23):13152-7.

  7. Rautou PE, Leroyer AS, Ramkhelawon B, et al. Microparticles From Human Atherosclerotic Plaques Promote Endothelial ICAM-1. Circ Res. 2011;108(3):335-43.

  8. Lin K, Hsu PP, Chen BP, et al. Molecular mechanism of endothelial growth arrest by laminar shear stress. PNAS. 2000,97(17):9385-9.

  9. Rhee WJ, Ni CW, Zheng Z, Chang K, Jo H, Bao G. HuR regulates the expression of stress-sensitive genes and mediates inflammatory response in human umbilical vein endothelial cells PNAS. 2010;107(15):6858-63.

  10. Shive MS, Salloum ML, Anderson JM. Shear stress-induced apoptosis of adherent neutrophils: A mechanism for persistence of cardiovascular device infections. PNAS. 2000;97(12):6710-5.

  11. Andersson ML, Karlsson PA, Svensson EU, et al. Differential global gene expression response patterns of human endothelium exposed to shear stress and intraluminal pressure. J Vasc Res. 2005;42(5):441-52.

  12. Kwon MC, Kong YY, Hwang IK, et al. TNF-Related Activation-Induced Cytokine of ICAM-1 and VCAM-1 via TNF enhances leukocyte adhesiveness: Induction receptor-associated factor and protein kinase C-dependent NF-kB activation in endothelial cell. J Immunol. 2005;175(1):531-40.

  13. Basit A, Reutershan J, Morris MA, Solga M, Rose CE Jr, Ley K. ICAM-1 and LFA-1 play critical roles in LPS-induced neutrophil recruitment into the alveolar space Am J Physiol Lung Cell Mol Physiol. 2006;291(2):L200-7.

  14. Burdick MM, Konstantopoulos K. Platelet-induced enhancement of LS174T colon carcinoma and THP-1 monocytoid cell adhesion to vascular endothelium under flow. Am J Physiol Cell Physiol. 2004;287(2): C539-547.

  15. Frommhold D, Kamphues A, Hepper I, Pruenster M. RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo. Blood. 2011;116 (5):841-9.

  16. Kim I, Moon SO, Kim SH, Kim HJ, Koh YS, Koh GY. Vascular endothelial growth factor activation in endothelial eells E-selectin through nuclear factor-kB adhesion molecule 1 (VCAM-1), and molecule 1 (ICAM-1), vascular cell expression of intercellular adhesion. J Biol Chem. 2001;276(10):7614-20.

  17. Ronald JA, Ionescu CV, Rogers KA, Sandig M. Differential regulation of transendothelial migration of THP-1 cells by ICAM-1/LFA-1 and VCAM-1/ VLA-4. J Leuko Biol. 2001;70(4):601-9.

  18. Muro S, Wiewrodt R, Thomas A, et al. A novel endocytic pathway induced by clustering endothelial ICAM-1 or PECAM-1. J Cell Sci. 2003;116(Pt 8):1599-609.

  19. Vogel J, Abounader R, Schröck H, Zeller K, Duelli R, Kuschinsky W. Parallel changes of blood flow and heterogeneity of capillary plasma perfusion in rat brains during hypocapnia. Am J Physiol. 1996;270(4 Pt 2):H1441-5.

  20. Vogel J, Kuschinsky W. Decreased heterogeneity of capillary plasma flow in the rat whisker-barrel cortex during functional hyperemia. J Cereb Blood Flow Metab. 1996;16 6):1300-6.

  21. Saucy F, Probst H, Alonso F, et al. Ex vivo pulsatile perfusion of human saphenous veins induces intimal hyperplasia and increased levels of the plasminogen activator inhibitor 1. Eur Surg Res. 2010;45(1):50-9.

  22. Dummler S, Eichhorn S, Tesche C, et al. Pulsatile ex vivo perfusion of human saphenous vein grafts under controlled pressure conditions increases MMP-2 expression. Biomed Eng Online. 2011;10:62.

  23. Montes-Sanchez D, Ventura JL, Mitre I, et al. Glycosylated VCAM-1 isoforms revealed in 2D western blots of HUVECs treated with tumoral soluble factors of breast cancer cells. BMC Chem Biol. 2009;9:7.

  24. Brenner W, Langer P, Oesch F, Edgell CJ, Wieser RJ. Tumor cell--endothelium adhesion in an artificial venule. Anal Biochem. 1995;225(2): 213-9.

  25. Makkar RR, Eigler NL, Kaul S, et al. Effects of clopidogrel, aspirin and combined therapy in a porcine ex vivo model of high-shear induced stent thrombosis. Eur Heart J. 1998;19(10):1538-46.

  26. Mavromatis K, Fukai T, Tate M, Chesler N, Ku DN, Galis ZS. Early effects of arterial hemodynamic conditions on human saphenous veins perfused ex vivo. Arterioscler Thromb Vasc Biol. 2000;20(8):1889-95.

  27. Eichhorn ME, Ney L, Massberg S, Goetz AE. Platelet kinetics in the pulmonary microcirculation in vivo assessed by intravital microscopy. J Vasc Res. 2002;39(4):330-9.

  28. Evensen L, Micklem DR, Blois A, et al. Mural cell associated VEGF is required for organotypic vessel formation. PLoS One. 2009;4(6):e5798.

  29. Thacher TN, Silacci TP, Stergiopulos N, da Silva RF. Autonomous effects of shear stress and cyclic circumferential stretch regarding endothelial dysfunction and oxidative stress: an ex vivo arterial model. J Vasc Res. 2010;47(4):336-45.

  30. May P, Arrouvel C, Revol M, Servant JM, Vicaut E. Detection of hemodynamic turbulence in experimental stenosis: an in vivo study in the rat carotid artery. J Vasc Res. 2002;39(1):21-9.

  31. Anwar MA, Shalhoub J, Lim CS, Gohel MS, Davies AH. The effect of pressure-induced mechanical stretch on vascular wall differential gene expression. J Vasc Res. 2012;49(6):463-78.

  32. Wang P, Zhu F, Lee NH, Konstantopoulos K. Shear-induced interleukin-6 synthesis in chondrocytes: roles of E prostanoid (EP) 2 and EP3 in cAMP/ protein kinase A- and PI3-K/Akt-dependent NF-kappaB activation. J Biol Chem. 2010;285(32):24793-804.

  33. Surapisitchat J, Hoefen RJ, Pi X, Yoshizumi M, Yan C, Berk BC. Fluid shear stress inhibits TNF-alpha activation of JNK but not ERK1/2 or p38 in human umbilical vein endothelial cells: Inhibitory crosstalk among MAPK family members. PNAS. 200;98(11):6476-81.

  34. Schenkel AR, Mamdouh Z, Muller WA. Locomotion of monocytes on endothelium is a critical step during extravasation. Nature Immunol. 2004;5(4):393-400.

  35. Ploppa A, Kampmann M, Johannes T, Haeberle HA, Nohé B. Effects of different leukocyte subpopulations and flow conditions on leukocyte accumulation during reperfusion. J Vasc Res. 2012;49(2):169-80.

  36. Calabrese EJ, Bachmann KA, Bailer AJ, et al. Biological stress response terminology. Toxicol Appl Pharm. 2007;222(1):122-8.

  37. Crook MF, Southgate KM, Newby AC. Both ICAM-1- and VCAM-1-Integrin interactions are important in mediating monocyte adhesion to human saphenous vein. J Vasc Res. 2002;39(3):221-9.

  38. Burns MP, DePaola N. Flow-conditioned HUVECs support clustered leukocyt adhesion by coexpressing ICAM-1 and E-selectin. Am J Physiol Heart Circ Physiol. 2005;288(1):H194-204.

>Journals >Gaceta Médica de México >Year 2015, Issue 2

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