>Gaceta Médica de México
>Year 2015, Issue 2
López-Macay A, Ruiz-Medina EJ, Ventura-Gallegos JL, Arechavaleta-Velasco F, Gómez-Quiroz LE, Konigsberg-Fainstein M, Zentella-Dehesa A
Characterization of hemodynamic ex vivo model to study endothelial activation by TNF-α in prefunded human veins
Gac Med Mex 2015; 151 (2)
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Inflammation is recognized as part of the etiology of numerous diseases. The interaction among cells of the immunological
system with local cells and molecules, such as cytokines and chemokines, allows cellular activation and response amplification.
The importance of several physicochemical factors like frictional force, vascular flow, shear stress, and pressure is now
recognized because they are known to modulate genetic expression and endothelial activation; however, there are very few
studies that recreate such cellular microenvironments. Hence, it is of paramount importance to develop new models that will mimic physiological conditions. Our aim was to improve a human vein ex vivo model that would allow endothelial activation
in flow conditions, to study the molecular components during adhesion, taking into consideration physicochemical parameters
such as flow and shear stress. Endothelial umbilical human vein was used and activated with TNF-α in order to determine
U937 monocytic cells adhesion, as well as cytokines secretion and ICAM-1 expression. This model will allow leukocyte
adhesion studies, using different inflammatory stimulus, along with the signaling pathways involved in several pathologies.
||Endothelium, Adhesion, Inflammation, Vascular flow, Perfusion.
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>Gaceta Médica de México
>Year 2015, Issue 2