Medicina Crítica

Contents by Year, Volume and Issue

Table of Contents

General Information

Instructions for Authors

Message to Editor

Editorial Board

>Journals >Medicina Crítica >Year 1999, Issue 5

Estrada GA, Guadarrama QF, Cortés AD, Franco GJ, Elizalde GJJ, Martínez SJ
Protection of acute lung injury due to oxygen exposure with N-acetylcisteine and exogenous surfactant in rats. Comparative study.
Rev Asoc Mex Med Crit y Ter Int 1999; 13 (5)

Language: Español
References: 12
Page: 155-159
PDF: 100.83 Kb.

Full text


Objective: To study the role of the protective effect of N-acetylcisteine and exogenous surfactant on the acute pulmonary injury (API) induced by oxygen.
Design: Prospective, comparative and descriptive study.
Setting: ICU of a teaching hospital of Mexico.
Subjects: Twelve male rats.
Interventions: All the animals were treatead by transtracheal route and were aleatory asigned to three groups: group I received N-acetylcisteine 40 mg/200 g, group II (control) was treated with saline solution 2 mL and group III received exogenous surfactant 200 mg/kg, previous exposure to oxygen (FIO2, 1.0) during eight hours. At the end of the experiment a pulmonar lobe was obtained. An expertise pathologist analyzed the tissues and classified APL according the lesion stage (I-VII) and the degree of severity injury (0-4 points).
Results: The percentage of injured rats was similar for the three groups at the initial (I-III) and at the end (V,VII) stages. The degree of severity injury was 2.5, 3.7 and 2.2 points for I, II and III groups respectively, in stage Ib (p ‹ 0.05). A significant difference was observed in stage VII between group II and III (3.5 vs. 1 points respectively); p = 0.01.
Conclusion: The salutary effects of N-acetylcisteine and surfactant are not conclusive in this study.

Key words: Acute pulmonary injury, N-acetylcisteine exogenous surfactant, oxygen exposure, rats.


  1. Piedoeuf B. In vivo expression of intracellular molecule-1 in type II pneumocytes during hyperoxia. AM J Resp Cell Mol Biol 1996;15:71-77.

  2. Walther FJ. Antioxidant-surfactant mitigate hyperoxic lung injury in premature rabbits. Am J Physiol 1995;18:598-602.

  3. Moller JC. Treatment of severe non-neonatal ARDS in children with surfactant and nitric oxide in a pre-ECMO situation. Int J Artif Org 1995;(18):598-602.

  4. Godínez MH. Inspired oxygen concentration alters the phospholipids and protein content in bronchoalveolar lavage accesible space. Crit Care Med 1996;24:862-869.

  5. Serje J. Exogenous surfactant preserve lung function and reduces alveolar Evans blue dye in flux in a rat model of ventilation-induced lung injury. Anesthesiology 1998;89: 464-474.

  6. Corbbet A. Double-blind, randomized trial of one versus three prophylactic dose of synthetic surfactant in 826 neonates weighing 700 to 100 grams, effects on mortality rate. American exsosurf neonatal study groups I and IIa. Biochem J 1995;309 (pt II):551-555.

  7. Pach ER. Prevention of intracellular adenosine triphosphate depletion after subletal oxidant injury to rat tipe II alveolar epithelial cells with exogenous glutation and N-acetylcisteine. Am J Med Sci 1995;310:133-137.

  8. Doyle IR. Serum surfactant protein-A levels in patients with acute cardiogenic pulmonary edema and adult respiratory distress syndrome. Am J Reps Crit Care Med 1995;152:7-17.

  9. Nieman GF. Surfactant replacement in the treatment of sepsis-induced adult respiratory distress syndrome in pigs. Crit Care Med 1996;24:1025-1033.

>Journals >Medicina Crítica >Year 1999, Issue 5

· Journal Index 
· Links 

Copyright 2019