1999, Number 5
Med Crit 1999; 13 (5)
Protection of acute lung injury due to oxygen exposure with N-acetylcisteine and exogenous surfactant in rats. Comparative study.
Estrada GA, Guadarrama QF, Cortés AD, Franco GJ, Elizalde GJJ, Martínez SJ
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ABSTRACTObjective: To study the role of the protective effect of N-acetylcisteine and exogenous surfactant on the acute pulmonary injury (API) induced by oxygen.
Design: Prospective, comparative and descriptive study.
Setting: ICU of a teaching hospital of Mexico.
Subjects: Twelve male rats.
Interventions: All the animals were treatead by transtracheal route and were aleatory asigned to three groups: group I received N-acetylcisteine 40 mg/200 g, group II (control) was treated with saline solution 2 mL and group III received exogenous surfactant 200 mg/kg, previous exposure to oxygen (FIO2, 1.0) during eight hours. At the end of the experiment a pulmonar lobe was obtained. An expertise pathologist analyzed the tissues and classified APL according the lesion stage (I-VII) and the degree of severity injury (0-4 points).
Results: The percentage of injured rats was similar for the three groups at the initial (I-III) and at the end (V,VII) stages. The degree of severity injury was 2.5, 3.7 and 2.2 points for I, II and III groups respectively, in stage Ib (p ‹ 0.05). A significant difference was observed in stage VII between group II and III (3.5 vs. 1 points respectively); p = 0.01.
Conclusion: The salutary effects of N-acetylcisteine and surfactant are not conclusive in this study.