>Revista de Hematología
>Year 2013, Issue 4
Gutiérrez-Aguirre CH, Salazar-Riojas R, Cantú-Rodríguez O, González-Llano Ó, Ríos-Solís JE, Gómez-Almaguer D
Efficacy and safety in the collection of hematopoietic stem cells from peripheral blood in pediatric donors using a central catether
Rev Hematol Mex 2013; 14 (4)
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Background: The apheresis procedure to obtain peripheral blood
hematopoietic stem cells is gradually replacing the bone marrow
harvest as the main procedure for obtaining cells for transplantation.
In pediatric donors, it is recommended to use a central line for this
procedure due to the advantages offered.
Objective: Analyze the results of procedures performed in pediatric
apheresis donors by venous access via a jugular central catheter.
Methods: We retrospectively analyzed the outcome of apheresis
procedures performed in pediatric donors of over 20 kg weight
through a 7.5 Fr dual lumen central jugular catheter placed by
ultrasound and fluoroscopy.
Results: Fifty-five donors were included, 36 for autologous transplant
and 19 for allogeneic transplant. In autologous transplantation
procedures, the median of blood volume processed, duration of
apheresis and CD34+ cells collected per kilo of weight receptor was
11,986 mL, 217 minutes and 4.4 x 106 respectively. In allogeneic
transplantation procedures, the median of blood volume processed,
duration of apheresis and CD34+ cells collected per kilo of weight
receptor was 15,014 ml, 230 minutes, and 7.6 x 106 respectively. No
complications related to central catheter placement or during the cell
collection procedure were observed. Eight patients required 2 apheresis
procedures in order to collect the desired amount of CD34+.
Conclusion: The stem cell collection procedure using a central
catheter guided by ultrasound and fluoroscopy is both effective and
safe in pediatric donors.
||Collection of hematopoietic cells, Peripheral blood, Ppediatric donors, Central catheter.
Moog R. Apheresis techniques for collection of peripheral blood progenitor cells. Transfusion and apheresis science. Journal of the European Society for Haemapheresis 2004;31:207-220.
Ottinger HD, Beelen DW, Scheulen B, Schaefer UW, Grosse- Wilde H. Improved immune reconstitution after allotransplantation of peripheral blood stem cells instead of bone marrow. Blood 1996;88:2775-2779.
Bensinger WI, Weaver C, Appelbaum F, et al. Transplantation of allogeneic peripheral blood stem cells mobilized by recombinant human granulocyte colony-stimulating factor. Blood 1995;85:1655-1658.
Korbling M, Dörken B, Ho AD, Pezzutto A, Hunstein W, Fliedner TM. Autologous transplantation of blood-derived hemopoietic stem cells after myeloablative therapy in a patient with Burkitt’s lymphoma. Blood 1986;67:529-532.
Korbling M, Przepiorka D, Huth YO, et al. Allogeneic blood stem cell transplantation for refractory leukemia and lymphoma: potential advantage of blood over marrow allografts [see comments]. Blood 1995;85:1659-1665.
Hölig K, Blechschmidt M, Kramer M, et al. Peripheral blood stem cell collection in allogeneic donors: impact of venous access. Transfusion 2012;52:2600-2605.
Kalantari K. The choice of vascular access for therapeutic apheresis. Journal of Clinical Apheresis 2012;27:153-159.
Sadler DJ, Gordon AC, Klassen J, Saliken JC, So CB, Gray RR. Image-guided central venous catheters for apheresis. Bone Marrow Transplant 1999;23:179-182.
Hahn U, Goldschmidt H, Salwender H, Haas R, Hunstein W. Large-bore central venous catheters for the collection of peripheral blood stem cells. J Clin Apher 1995;10:12- 16.
Moog R. Peripheral blood stem cell collection in children: Management, techniques and safety. Transfusion and apheresis science. Journal of the European Society for Haemapheresis 2010;43:203-205.
Galacki, D.M., An overview of therapeutic apheresis in pediatrics. J Clin Apher 1997;12:1-3.
Rinaldi C, Savignano C, Pasca S, et al. Efficacy and safety of peripheral blood stem cell mobilization and collection: a single-center experience in 190 allogeneic donors. Transfusion 2012;52:2387-2394.
Baldomero H, Gratwohl M, Tichelli A, et al. The EBMT activity survey 2009: trends over the past 5 years. Bone Marrow Transplant 2011;46:485-501.
Marson P, Petris MG, De Silvestro G. Collection of peripheral blood stem cells in pediatric patients: a concise review on technical aspects. Bone Marrow Transplant 1998 22 Suppl 5:S7-11.
Han X, Ma L, Zhao L, et al. Predictive factors for inadequate stem cell mobilization in Chinese patients with NHL and HL: 14-year experience of a single-center study. Journal of Clinical Apheresis 2012;27:64-74.
Sung AD, Grima DT, Bernard LM, et al. Outcomes and costs of autologous stem cell mobilization with chemotherapy plus G-CSF vs G-CSF alone. Bone Marrow Transplantation 2013;48:1444-1449.
>Revista de Hematología
>Year 2013, Issue 4