>Year 1997, Issue 3
Alaniz-de la O R, Ríos-Ibarra ML, Rosas-Barbosa BT, Juan-Morales AL
Antimicrobial resistance of Salmonella strains isolated from animal sources
Vet Mex 1997; 28 (3)
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The frequency of resistance to different antimicrobials was studied in 122 strains of Salmonella obtained from bovine feces, animal excreta recycled for animal feeding and in bovine mesenteric lymph nodes. Strains were tested for resistance to 10 antimicrobials by the disk diffusion method described by the National Committee for Clinical Laboratory Standards. Resistance to one or more antimicrobials was observed in 73 (59.8%) of the tested strains. Forty-nine (67.1%) of these strains showed multiple resistance. Resistance to at least one of the tested antimicrobials was found in 26.3% of the strains from bovine feces, 69% from recycled manure, and 59.4% from lymph nodes. Resistance to tetracycline (57.4%) was the most commonly detected, followed by streptomycin (22.1 %) and chloramphenicol (20.5%). No strains exhibited resistance to cephalothin or nalidixic acid. Of the 16 resistance patterns identified, 14 (87.5%) contained a resistance determinant for tetracycline and 10 (62.5%) for streptomycin. Twenty out of the 25 examined serovars of Salmonella showed resistance to at least one of the tested antimicrobials, and 15 showed multiple resistance. Results emphasize the need to regulate the use of antimicrobials in animal husbandry in Mexico, as well as improve a program for monitoring the drug resistance in Salmonella isolates from different sources in an attempt to identify the trends of antimicrobial resistance and control its dissemination.
||ANIMAL, ANTIMICROBIAL RESISTANCE, SALMONELLA.
Bauer, A.W., Kirby, M.M., Sherris, J.C. and Turk, M.: Antibiotic susceptibility testing by a standardized single disk method. Am.., 45: 493-496 (1966).
Bezanson, G.S., Pauzé, M. and Lior, H.: Antibiotic resistance and R-plasmids in food chain Salmonella: Evidence of plasmid relatedness. Appl. Environ. Microbiol., 41:585-592 (1981).
Blackburn, O., Schlater, L.K. and Swanson, M.R.: Antibiotic resistance of members of the genus Salmonella isolated from chickens, turkeys, cattle and swine in the United States during October 1981 through September 1982. Am. J. vet. Res., 45: 1245-1249 (1984).
Carvalho, C.L. and Hofer, E.: Antimicrobial resistance among Salmonella serovars isolated from different sources in Brazil during 1978-1983. Antonie Leeuwenhoek, 55: 349-359 (1989).
Cohen, M.L. and Tauxe, R.V.: Drug-resistant Salmonella in the United States: An epidemiologic perspective. Science, 234: 964-969 (1986).
D'Aoust, J.Y.: Pathogenicity of foodborne Salmonella. Int. J. Food Microbiol., 12: 17-40 (1991).
D'Aoust, J.Y., Sewell, A.M., Daley, E. and Greco, P.: Antibiotic resistance of agricultural and foodborne Salmonella isolates in Canada: 1986-1989. J. Food Prot., 55: 428-434 (1992).
Duck, P.D., Dillon, J.R., Lior, H. and Eidus, L.: Antibiotic resistance among predominant Salmonella serovars and phagovars in Canada. Can. J. Microbiol., 24: 1358-1365 (1978).
DuPont, H.L. and Steele, J.H.: Use of antimicrobial agents in animal feeds: Implications for human health. Rev. Infect. Dis., 9: 447-460 (1987).
Hadfield, T.L., Monson, M.H. and Wachsmuth, I.K.: An outbreak of antibiotic-resistant Salmonella enteritidis in Liberia, West Africa. J. Infect. Dis., 151: 790-795 (1985).
Heffernan, H.M.: Antibiotic resistance among Salmonella from human and other sources in New Zealand. Epidemiol. Infect., 106: 17-23 (1991).
Holmberg, S.D., Osterholm, M.T., Senger, K.A. and Cohen, M.L.: Drug-resistant Salmonella from animals fed antimicrobials. N. Engl. J. Med., 311: 617-622 (1984).
Holmberg, S.D., Solomon, S.L. and Blake, P.A.: Health and economic impacts of antimicrobial resistance. Rev. Infect. Dis., 9: 1065-1078 (1987).
Holmberg, S.D., Wells, J.G. and Cohen, M.L.: Animal-to-man transmission of antibiotic-resistant Salmonella: Investigations of U.S. outbreaks, 1971-1983. Science, 225: 833-835 (1984).
Instituto Nacional de Diagnóstico y Referencia Epidemiológicos: Serotipificación de Especies de Salmonella Aisladas en el INDRE (México) 1982-1992. Secretaría de salud, México, D.F., 1993.
Levy, S.B.: Antibiotic use for growth promotiorr in animals: Ecologic and public health consequences. J. Food Prot., 50: 616-620 (1987).
Lyons, R.W., Samples, C.L., DeSilva, H.N., Ross, K.A., Julian, E.M. and Checko, P.J.: An epidemic of resistant Salmonella in a nursery. J. Am. med. Ass., 243:546-547 (1980).
MacDonald, K.L., Cohen, M.L., Hargrett-Bean, N.T., Wells, J.G., Puhr, N.D., Collin, S.F. and Blake, P.A.: Changes in antimicrobial resistance of Salmonella isolated from humans in the United States. J. Am. med. Ass., 258: 1496-1499 (1987).
National Committee for Clinical Laboratory Standards: Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved Standard M2-A4. 4th ed. National Committee for Clinical Laboratory Standards, Villanova, Philadelphia, 1990.
Neu, H.C., Cherubin, C.E., Longo, E.D., Flouton, B. and Winter, J.: Antimicrobial resistance and R-Factor transfer among isolates of Salmonella in the Northeastern United States: A comparison of human and animal isolates. J. Infect. Dis., 132: 617-622 (1975).
Neu, H.C.: The crisis in antibiotic resistance. Science, 257: 1064-1073 (1992).
Rangnekar, V.M., Banker, D.D. and Jhala, H.I.: Antimicrobial resistance and incompatibility groups of R plasmids in Salmonella typhimurium isolated from human sources in Bombay from 1978 to 1980. Antimicrob. Agents Chemother., 23: 54-58 (1983).
Riley, L.W., Ceballos, B.S.O., Trabulsi, L.R, Fernandez-de Toledo, M.R. and Blake, P.A.: The significance of hospitals as reservoirs for endemic multiresistant Salmonella typhimurium causing infections in urban Brazilian children. J. Infect. Dis., 150: 236-241 (1984).
Ryan, C.A., Nickels, M.K., Hargrett-Bean, N.T., Potter, M.E., Endo, T., Mayer, L., Langkop, C.W., Gibson, C., McDonald, R.C., Kenney, R.T., Puhr, N.D., McDonneli, P.J., Martin, R.J., Cohen, M.L. and Blake, P.A.: Massive outbreak of antimicrobial-resistant salmonellosis traced to pasteurized milk. J. Am. med Ass., 258: 3269-3274 (1987).
Ryder, R.W., Blake, P.A., Murlin, A.C., Carter, G.P., Pollard, R.A., Merson, M.H., Allen, S.D. and Brenner, D.J.: Increase in antibiotic resistance among isolates of Salmonella in the United States, 1967-1975. J. Infect. Dis., 142: 485-491 (1980).
Spika, J.S., Waterman, S.H., Soo Hoo, G.W., St. Louis, M.E., Pacer, R.E., James, S.M., Bissett, M.L., Mayer, L.W., Chiu, J.Y., Hall, B., Greene, K., Potter, M.E., Cohen, M.L. and Blake, P.A.: Chloramphenicol-resistant Salmonella newport traced through hamburger to dairy farms. N. Engl. J. Med., 316: 565-570 (1987).
Tauxe, R.V.: Salmonella: A postmodern pathogen. J. Food Prot., 54: 563-568 (1991).
Threlfall, E.J., Ward L.R., Ashley, A.S. and Rowe, B.: Plasmidencoded trimethoprim resistance in multiresistant epidemic Salmonella typhimurium phage types 204 and 193 in Britain. Br. med. J., 280: 1210-1211 (1980).
>Year 1997, Issue 3