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>Journals >Veterinaria México >Year 2012, Issue 2

Noro M, Wittwer F
Relationships between liver ureagenesis and gluconeogenesis in ruminants fed with a high nitrogen diet
Vet Mex 2012; 43 (2)

Language: English/Spanish
References: 63
Page: 143-154
PDF: 475.55 Kb.

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Liver ureagenesis and gluconeogenesis are essential metabolic functions in ruminants. These metabolic processes are more intensive in ruminants than other mammals, especially on grazing ruminants or those supplemented with nitrogen because they absorb large amount of the ammonia produced in the rumen and only small amounts of the intestinal glucose. Diets based on the use of forages with a high content of crude protein, rapidly degradable in the rumen, or the supplementation with non-protein nitrogen, increases the ruminal production of ammonia. The excess of ammonia is absorbed and metabolized to urea by the liver. The production of large amounts of ammonia in the rumen and its subsequent absorption can exceed the liver capacity to metabolize urea causing an overcharge in the urea cycle, demanding a large amount of α-ketoglutarate and oxalacetate to produce glutamate and aspartate. Under this condition the gluconeogenic capacity of the organism is altered because these metabolites are also required in the Krebs cycle and gluconeogenesis. The relationships between liver ureagenesis and gluconeogenesis in ruminants fed a high content of nitrogen are reviewed.

Key words: Ureagenesis, gluconeogenesis, non protein nitrogen, ruminants.


  1. HUNTINGTON GB, ARCHIBEQUE SL. Practical aspects of urea and ammonia metabolism in ruminants. J Anim Sci. 2000; 78: 742:749.

  2. REYNOLDS CK. Metabolism of nitrogenous compounds by ruminant liver. J Nutr 1992; 122: 850-854.

  3. BERGMAN EN, HOGUE DE. Glucose turnover and oxidation rates in lactating sheep. Am J Physiol 1967; 213: 1378-1384.

  4. SINCLAIR KD, SINCLAIR LA, ROBINSON JJ. Nitrogen metabolism and fertility in cattle: I. Adaptative changes in intake and metabolism to diets differing in their rate of energy and nitrogen release in the rumen. J Anim Sci 2000; 78: 2659-2669.

  5. MOORE DA, VARGA G. BUN and MUN: Urea nitrogen testing in dairy cattle. Compendium 1996; 18: 712-720.

  6. HALIBURTON JC, MORGAN SE. Nonprotein nitrogen-induced ammonia toxicosis and ammoniated feed toxicity syndrome. Vet Clin North Am: Food Anim Pract 1989; 5: 237-249.

  7. VISEK WJ. Ammonia: Its effects on biological systems, metabolic hormones, and reproduction. J Dairy Sci 1984; 67: 481-498.

  8. ANTONELLI AC, TORRES GAS, MORI CS, SOARES PC, MARUTA CA, ORTOLANI EL. Intoxicação por amônia em bovinos que receberam uréia extrusada ou granulada: alterações em alguns componentes bioquímicos do sangue. Braz J Vet Res Anim Sci 2009; 46: 69-76.

  9. GUSTAFFSON AH, PALMQUIST DL. Diurnal variation of rumen ammonia, serum urea, and milk urea in dairy cows at high and low yields. J Anim Sci 1993; 76: 475-484.

  10. MACKLE TR, PARR CR, BRYANT AM. Nitrogen fertilizer effects on milk yield and composition, pasture intake, nitrogen and energy partitioning, and rumen fermentation parameters of dairy cows in early lactation. New Zel J Agric Res 1996; 39: 341-356.

  11. HENNING PH, STEYN DG, MEISSNER HH. Effect of synchronization of energy and nitrogen supply on ruminal characteristics and microbial growth. J Anim Sci 1993; 71: 2516-2528.

  12. VISEK WJ. Ammonia metabolism, urea cycle capacity and their biochemical assessment. Nutr Rev 1979; 37: 273-282.

  13. MAZZAFERRO E, HACKETT T, WINGFIELD W, OGILVIE G, FETTMAN M. Role of glutamine in health and disease. Compendium 2000; 22: 1094-1103.

  14. KATZ NR. Metabolic Heterogeneity of hepatocytes across the liver acinus. J Nutr 1992; 122: 843-849.

  15. LOBLEY GE, MILANO GD, VAN DER WALT JG. The liver: Integrator of nitrogen metabolism. In: CRONJE P B, editor. Ruminant Physiology: Digestion, Metabolism, Growth and Reproduction. Pretoria, South Africa:CABI, 2000: 149-168.

  16. HÄUSSINGER D. Nitrogen metabolism in liver: structural and functional organization and physiological relevance. Biochem J 1990; 267: 281-290.

  17. BAREJ W. On extent of ureagenesis and gluconeogenesis in ruminants with regards to the diet. Arch Anim Nutr 1986; 40: 154-163.

  18. NOLAN JV, NORTON BW, LENG RA. Further studies of the dynamics of nitrogen metabolism in sheep. Br J Nutr 1976; 35: 127-147.

  19. RODWELL VW. Catabolism of proteins and of amino acid nitrogen. In: MURRAY RK, GRANNER DK, MAYES PA, RODWELL VW, editors. Harper´s Biochemistry. 25th ed. New York, USA: MacGraw-Hill, 2000: 313-322.

  20. MADSEN A. The molecular basis of animal production: Metabolism in liver cells. In: RIIS PM, editor. Dynamic Biochemistry of Animal Production. New York, USA: Elsevier, 1983: 53-74.

  21. LUO QJ, MALTBY SA, LOBLEY GE, CALDER AG, LOMAX MA. The effect of amino acids on the metabolic fate of 15NH4Cl in isolated sheep hepatocytes. Eur J Biochem 1995; 228: 912-917.

  22. HARTWELL JR, CECAVA MJ, DONKIN SS. Rumen undegradable protein, rumen-protected choline and mRNA expression for enzymes in gluconeogenesis and ureagenesis in periparturient dairy cows. J Dairy Sci 2001; 84: 490-497.

  23. VISEK WJ. Some aspects of ammonia toxicity in animal cells. J Dairy Sci 1968; 51: 286-295.

  24. 24 LOBLEY GE, CONNELL A, LOMAX MA, BROWN DS, MILNE E, CALDER AG et al. Hepatic detoxification of ammonia in the ovine liver: possible consequences for amino acid catabolism. Br J Nutr 1995; 73: 667-685.

  25. LOBLEY GE, BREMNER DM, NIETO R, OBITSU T, MOORE AH, BROWN DS. Transfers of N metabolites across the ovine liver in response to short-term infusions of an amino acid mixture into the mesenteric vein. Br J Nutr 1998; 80: 371-379.

  26. WHITT J, HUNTINGTON G, ZETINA E, CASSE E, TANIGUCHI K, POTTS W. Plasma flow and net nutrient flux across gut and liver of cattle fed twice daily. J Anim Sci 1996; 74: 2450-2461.

  27. MUTSVANGWA T, BUCHANAN-SMITH JG, MCBRIDE BW. Effects of ruminally degradable nitrogen intake and in vitro addition of ammonia and propionate on the metabolic fate of L-[1-14C Alanine and L-[15N Alanine in isolated sheep hepatocytes. J Anim Sci 1997; 75: 1149-1159.

  28. LOBLEY GE, BREMNER DM, BROWN DS. Response in hepatic removal of amino acids by the sheep to short-term infusions of varied amounts of an amino acid mixture into the mesenteric vein. Br J Nutr 2001; 85: 689-698.

  29. FICO ME, MOTYL T, MILNER JA. Species comparison of the influence of ammonia on orotic acid and urea biosynthesis in liver. J Nutr 1984; 114: 613-621.

  30. FERNANDEZ JM, CROOM JR WJ, JOHNSON AD, JAQUETTE RD, EDENS FW. Subclinical ammonia toxicity in steers: effects on blood metabolite and regulatory hormone concentrations. J Anim Sci 1988; 66: 3259-3266.

  31. KAYE MM, FERNANDEZ JM, WILLIAMS CC, WHITE TW, WALKER RL. Differential responses to an oral urea load test in small ruminants: species and breed effects. Small Rum Res 2001; 42: 211-217.

  32. FERNANDEZ JM, CROOM WJJ, TATE LP, JOHNSON AD. Subclinical ammonia toxicity in steers: effects on hepatic and portal-drained visceral flux of metabolites and regulatory hormones. J Anim Sci 1990; 68: 1726-1742.

  33. CHOUNG JJ, CHAMBERLAIN DG. Effects of intraruminal infusion of propionate on the concentrations of ammonia and insulin in peripheral blood of cows receiving an intraruminal infusion of urea. J Dairy Res 1995; 62: 549-557.

  34. FERNANDEZ J, SAHLU T, HART S, POTCHOIBA M, EL SHAER H, JACQUEMET N et al. Experimentallyinduced subclinical hyperammonemia in dairy goats. Small Rum Res 2001; 42: 5-20.

  35. BRITO AL. Avaliação do uso intensivo de cama de frango na alimentação de bovinos: Alguns aspectos toxicológicos e do metabolismo do nitrogênio (tese doutorado). São Paulo, Brasil: Universidade de São Paulo-USP, 1999.

  36. ROLLER MH, RIEDEMANN GS, ROMKEMA GE, SWANSON RN. Ovine blood chemistry values measured during ammonia toxicosis. Am J Vet Res 1982; 43: 1068-1071.

  37. SYMONDS HW, MATHER DL, COLLIS KA. The maximum capacity of the liver of the adult dairy cow to metabolize ammonia. Br J Nutr 1981; 46: 481-486.

  38. GRAHAM NM. Units of metabolic body size for comparisons amongst adult sheep and cattle. Proceedings of Australian Society of Animal Production, Ninth Biennial Conference. 1972 Feb 13-19; Canberra, Australia: Ramsay Were Publishing Pty Ltd. 1972; 9: 352-355.

  39. MAYES P. Gluconeogenesis and control of the blood glucose. In: MURRAY RK, GRANNER DK, MAYES PA, RODWELL VW, editors. 25th ed. New York, USA: MacGraw-Hill, 2000: 208-218.

  40. HERDT TH. Fuel homeostasis in the ruminant. Vet Clin North Am: Food Anim Pract 1988; 4: 213-231.

  41. HERDT TH. Ruminant adaptation to negative energy balance. Influences on the etiology of ketosis and fatty liver. Vet Clin North Am Food Anim Pract 2000; 16: 215-230.

  42. KOLB E. Fisiología Veterinaria. Vol. II. Zaragoza, España: Acribia, 1991.

  43. TELENI E, ANNISON EF. Release of gluconeogenic amino acids from hind-limb muscles of fed and starved sheep. In: BAKER SK, GAWTHORNE JM, MACKINTOSH JB, PURSER DB, editors. Ruminant physiology: concepts and consequences: a tribute to R. J. Moir. Perth, Australia: University of Western Australia, 1984.

  44. GONZÁLEZ FD, SILVA SC. Introdução à bioquímica clínica veterinária, 2ª Ed. Porto Alegre RS-Brasil, Universidade Federal do Rio Grande do Sul, 2006.

  45. BERGMAN EN, STARR DJ, REULEIN SSJ. Glycerol metabolism and gluconeogenesis in the normal and hypoglycemic ketonic sheep. Am J Physiol 1968; 215: 874-880.

  46. CHALUPA W, SNIFFEN CJ. Protein and amino acid nutrition of lactating dairy cattle. Vet Clin North Am: Food An Prac 1991; 7: 353-372.

  47. FORD EJ, REILLY PE. The utilization of plasma free amino acid and glucose carbon by sheep. Res Vet Sci 1969; 10: 409-418.

  48. OVERTON TR, DRACKLEY JK, OTTEMANNABBAMONTE CJ, BEAUIEU AD, EMMERT LSC. Substrate utilization for hepatic gluconeogenesis is altered by increased glucose demand in ruminats. J Anim Sci 1999; 77: 1940-1951.

  49. MILLS SE, BEITZ DC,YOUNG JW. Evidence for impaired metabolism in liver during induced lactation ketosis of dairy cows. J Dairy Sci 1986; 69: 362-370.

  50. YANEZ AJ, BERTINAT R, CONCHA, II, SLEBE JC. Nuclear localization of liver FBPase isoenzyme in kidney and liver. FEBS Lett 2003; 550: 35-40.

  51. SAEZ DE, FIGUEROA CD, CONCHA II, SLEBE JC. Localization of the fructose 1,6-bisphosphatase at the nuclear periphery. J Cell Biochem 1996; 63: 453-462.

  52. YANEZ AJ, GARCIA-ROCHA M, BERTINAT R, DROPPELMANN C, CONCHA II, GUINOVART JJ et al. Subcellular localization of liver FBPase is modulated by metabolic conditions. FEBS Lett 2004; 577: 154-158.

  53. DEMIGNÉ C, YACOUB C, RÉMÉSY C, FAFOURNOUX P. Propionate and butyrate in rat or sheep hepatocytes. Bioch Bioph Acta 1986; 875: 535-542.

  54. MEIJER AJ, GIMPEL JA, DELEEUW G, TISCHLER ME, TAGER JM , WILLIAMSON JR. Interrelatioships between gluconeogenesis and ureogenesis in isolated hepatocytes. J Biol Chem 1978; 253: 2308-2320.

  55. ORZECHOWSKI A, PIERZYNOWSKI S, MOTYL T, BAREJ W. Net hepatic metabolism of ammonia, propionate and lactate in sheep in relation to gluconeogenesis and ureagenesis. J Anim Physiol Anim Nutr 1988; 59: 113-122.

  56. PARKER D, LOMAX M, SEAL C, WILTON J. Metabolic implications of ammonia production in the ruminant. Proc Nutr Soc 1995; 54: 549-563.

  57. NORO M. Gluconeogénesis hepática en ovinos (Ovis aries) alimentados con una dieta alta en nitrógeno no proteico (tesis doctoral). Valdivia, Chile: Universidad Austral de Chile, 2006.

  58. NORO M, BARBOZA CS, BENITEZ O, PULIDO R, WITTWER F. Capacidad gluconeogénica vía propionato en vacas lecheras pastoreando alta o moderada oferta de pradera y suplementadas con dos fuentes de nitrógeno, XXXV Reunión Anual SOCHIPA; 2010 octubre 27-29; Coyhaique, Chile, Coyhaique, INIA Tamel Aike, 2010: 137-138.

  59. NORO M, WITTWER P, SCANDOLO D, GONZALEZ C, HARO F, SANDOVAL A. Effect of non-protein nitrogen supplementation on glucose and urea syntheses after a propionate loading test in sheep. Vet Clin Path 2005; 34: 305.

  60. CHALUPA W, CLARK J, OPLIGER P, LAVKER R. Detoxication of ammonia in sheep fed soy protein or urea. J Nutr 1970; 100: 170-176.

  61. MARINI JC, KLEIN JD, SANDS JM, VAN AMBURGH ME. Effect of nitrogen intake on nitrogen recycling and urea transporter abundance in lambs. J Anim Sci 2004; 82: 1157-1164.

  62. NEWSHOLME E, LEECH A. Biochemistry for the Medical Sciences. Chichester, UK: John Wiley & Sans Inc, 1983.

  63. NEWSHOLME P, LIMA MM, PROCOPIO J, PITHONCURI TC, DOI SQ, BAZOTTE RB et al. Glutamine and glutamate as vital metabolites. Braz J Med Biol Res 2003; 36: 153-163.

>Journals >Veterinaria México >Year 2012, Issue 2

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