Table 3: Preparation of technical personnel

before performing the DLCOsb test.

1. Barrier devices and cleaning procedures:

– Use of barrier devices, such as filters, should be made to prevent cross transmission of diseases

– Despite the use of in-line filters, the ATS and ALAT standards stress the continuous need for regular cleaning and decontamination of equipment

2. Personal hygiene:

– Health personnel should follow the recommended standards of hand washing, either by hand washing (40-60 seconds) or by rubbing with alcohol gel (20-30 seconds)

– The 5 moments of hand washing/hand hygiene, according to World Health Organization (WHO) guidelines, should be followed

3. Personal Protective Equipment (PPE):

– PPE includes gown for activities that may generate splashes or sprayable liquids from blood, organic fluids, secretions or excretions

– Use of disposable gloves in procedures with potential contact with infectious material, changing them between tasks and procedures, and washing gloves with alcohol gel before removing them

– Use of surgical mask or N95 during patient care, and eye protection to prevent splashes, in case of contagious diseases

4. Disinfection and sterilization:

– Before caring for each patient, disinfection and/or sterilization of equipment, instruments and surfaces must be carried out

These measures, based on ALAT (Latin American Thorax Association) recommendations, are essential to ensure a safe testing environment and minimize the risk of disease transmission.20

ATS = American Thoracic Society.

DLCOsb = single-breath pulmonary diffusion of carbon monoxide.