1997, Number 1
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Vet Mex 1997; 28 (1)
DIG-ELISA: Standardization and serodiagnostic evaluation in experimental and natural bovine fascioliasis
Ibarra VF, Montenegro CN, Vera MY, Boulard C, Quiroz RH, Bautista GCR, Vázquez PC
Language: English/Spanish
References: 23
Page: 7-12
PDF size: 459.74 Kb.
ABSTRACT
The aims of the study were to determine the sensitivity and specificity of the DIG-ELISA test and to establish the cut-off point value to carry out diagnosis of bovine fascioliasis; to determine the diagnostic capability of the test in artificially infected cattle; to evaluate the seroprevalence during one year of the IgG anti-
F. hepatica titrers of sera from naturally infected bovines, and to compare its variation with the presence of
Fasciola eggs in the coproparasitoscopic analysis. To standardize the test, eighty sera of adult cattle were used. Forty were from the state of Sonora (free fluke area) and forty from the state of Hidalgo (enzootic area for fascioliasis). The test showed 97.5% sensitivity and 80.0% specificity. The cut-off point value was 7 mm. In another study, fifteen young bovines were infected each with 600 metacercariae of
F. hepatica. To obtain sera, animals were bled weekly for 14 weeks. The test detected antibodies anti-
F. hepatica from the second week after infection, showing its major increments in diameter of the zone of reaction (DZR) between the seventh and the tenth week. In another experiment, eighty five crossbred cows located in an enzootic area for fascioliasis were used. They were sampled to obtain sera and faeces every two months for one year to be analyzed similarly to the previous study. Seroprevalence obtained were: 58%,73%,80%,100%,96% and 79% for the months of March, May, July, September, January and March, respectively, showing similarity in prevalence to the data obtained with the coproparasitoscopic analysis (P ‹ 0.01). DIGELISA showed high sensitivity and easy management in the laboratory, therefore, recommended for use under field conditions for early diagnosis of bovine fascioliasis, generating reproductible results which can be of great use for epidemiological studies.
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