2022, Número 1
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TIP Rev Esp Cienc Quim Biol 2022; 25 (1)
Actividad antimicrobiana de Streptomyces sp. Y15 contra bacterias patógenas y evaluación de medios de cultivo para la producción de antibióticos
Evangelista-Martínez Z, Ríos-Muñiz DE, Gómez-Cano J, Montoya-Hidalgo AC, Ochoa-Solórzano RE
Idioma: Ingles.
Referencias bibliográficas: 48
Paginas: 1-12
Archivo PDF: 1373.51 Kb.
RESUMEN
Una característica notable de las bacterias
Streptomyces es la capacidad para producir metabolitos secundarios. El objetivo del
estudio fue identificar estreptomicetos con actividad antibacteriana y usarlos contra bacterias patógenas de plantas y humanos,
así como evaluar la producción de antibióticos en diferentes medios de cultivo. Para seleccionar estreptomicetos con actividad
antibacteriana, se empleó el método de difusión de disco de agar en 31 aislados, de los que fue seleccionado un aislado, que
posteriormente se cultivó en nueve medios para evaluar la producción de metabolitos inhibitorios. Se detectaron tres aislados con
actividad inhibitoria contra al menos cuatro especies patógenas. El estreptomiceto nombrado Y15 presentó actividad antibacteriana
contra
Escherichia coli, Staphylococcus epidermidis, Listeria monocytogenes, Staphylococcus aureus, Pseudomonas syringae pv.
phaseolicola, Dickeya dadantii, y
Pectobacterium carotovorum. La producción de los metabolitos antimicrobianos se mantuvo
cuando Y15 creció en un medio con limitada concentración de fuente de nitrógeno. La presencia de compuestos con nitrógeno
orgánico provocó una disminución en la producción de los metabolitos antibacterianos. El aislado Y15 se identificó como
miembro del género
Streptomyces mediante la secuencia del gen 16S rDNA. La composición del medio de fermentación es un
factor importante para modular la producción de antibióticos, que tiene impacto sobre la cantidad y diversidad de los metabolitos
secundarios producidos.
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