medigraphic.com

2016, Número 1

<< Anterior

Vet Mex 2016; 3 (1)


El Mycobacterium avium subesp. paratuberculosis disminuye la regulación de la expresión del ARNm de la ferroportina 1 en los macrófagos inducidos con hierro

Landeros-Sánchez B, Gutierrez-Pabello JA, Medina-Basulto GE, Renteria-Evangelista TB, Díaz-Aparicio E, Oshima S

Idioma: Español/Inglés
Referencias bibliográficas: 26
Paginas: 1-12
Archivo PDF: 369.33 Kb.


RESUMEN

El Mycobacterium avium subsp. paratuberculosis (map) es el agente causal de la enfermedad de Johne. Los mecanismos por los cuales map es capaz de adaptarse a la respuesta natural del huésped aún no están claros. A través de la pcr en tiempo real, se examinaron los niveles de expresión del arn de la ferroportina 1 (fpn1) en células de macrófagos de ratón J774 infectados con Mycobacterium avium subsp. paratuberculosis o con el extracto crudo de proteína (ecp) de map. La multiplicidad de infección (moi) con map dependió de la disminución de los niveles del arnm de la fpn1. Los macrófagos infectados con las moi de 20:1 y 15:1 no mostraron cambio alguno en la expresión del gen fpn1, mientras que en las moi de 10:1 y 5:1, disminuyó en un 50% y 80%, respectivamente. En los macrófagos tratados con 50, 100, 150 y 200 µg/mL de ECP de map (ATCC19698), la expresión del arnm de fpn1 disminuyó 25%. La sobreexpresión del arnm de fpn1 en macrófagos J774 tratados con una sobrecarga de hierro de 400 µM de nitrilotriacetato férrico (fenta) se suprimió cerca de un 70% por la infección con map viva (moi 20:1). Estos datos revelaron que map inhibe la expresión del arnm de fpn1, lo que sugiere que existe un mecanismo bacteriano que participa en la regulación del hierro del huésped.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. 1) Abboud S, Haile DJ. 2000. A novel mammalian iron-regulated protein involved in intracellular iron metabolism. Journal Biological Chemistry, 275:19906-19912.

  2. 2) Ciaramella A, Cavone A, Santucci MB, Garg SK, Sanarico N, Bocchino M, Galati D, Martino A, Auricchio G, D’Orazio M, Stewart GR, Neyrolles O, Young DB, Colizzi V, Fraziano M. 2004. Induction of apoptosis and release of interleukin-1β by cell wall-associated 19-kDa lipoprotein during the course of mycobacterial infection. Journal of Infectious Diseases, 190(6):1167-1176. doi:10.1086/423850

  3. 3) Donovan A, Brownlie A, Zhou Y, Shepard J, Pratt SJ, Moynihan J, Paw BH, Drejer A, Barut B, Zapata A, Law TC, Brugnara C, Lux SE, Pinkus GS, Pinkus JL, Kingsley PD, Palis J, Fleming MD, Andrews NC, Zon LI. 2000. Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter. Nature, 403:776-781.

  4. 4) Eckelt E, Jarek M, Frömke C, Meens J and Goethe R. 2014. Identification of a lineage specific zinc responsive genomic island in Mycobacterium avium ssp. paratuberculosis. BMC Genomics, 15:1076.

  5. 5) Eckelt E, Meißner T, Meens J, Laarmann K, Nerlich A, Jarek M , Weiss S, Gerlach G and Goethe R. 2015. FurA contributes to the oxidative stress response regulation of Mycobacterium avium ssp. paratuberculosis. Frontiers in Microbiology, 6:16.

  6. 6) Ganz Tomas. 2009. Iron in innate immunity: Starve the invaders. Current Opinion in Immunology, 21:63-67.

  7. 7) Janagama H, Senthilkumar TM, Bannantine JP, Rodriguez GM, Smith I, Paustian ML, McGarvey JA, Sreevatsan S. 2009. Identification and functional characterization of the iron-dependent regulator (IdeR) of Mycobacterium avium subsp. paratuberculosis. Microbiology, 155:3683-3690.

  8. 8) Janagama HK, Senthilkumar TM, Bannantine JP, Kugadas A, Jagtap P, Higgins LA, Witthuhn BA, Sreevatsan S. 2010. Iron-sparing response of Mycobacterium avium subsp. paratuberculosis is strain dependent. BMC Microbiology, 10:268-278.

  9. 9) Krewulak KD, Vogel HJ. 2008. Structural biology of bacterial iron uptake. Biochimia et Biophysica Acta-Biomembanes, 1778:1781-1804.

  10. 10) Lamont E, Wayne Xu, and Srinand S. 2013. Host-Mycobacterium avium subsp. paratuberculosis interactome reveals a novel iron assimilation mechanism linked to nitric oxide stress during early infection. BMC Genomics, 14:694.

  11. 11) Lee J, Remold HG, Ieong MH, Kornfeld H. 2006. Macrophage apoptosis in response to high intracellular burden of Mycobacterium tuberculosis is mediated by a novel caspase-independent pathway. Journal of Immunology. 176:4267-4274.

  12. 12) Li L, Bannantine J, Zhang Q, Amonsin A, May B, Alt D, Benerji N, Kanjilal S, Kapur V. 2005. The complete genome sequence of Mycobacterium avium subsp. paratuberculosis. Proceedings of the National Academy of Sciences, 102:12344-12349.

  13. 13) Li W, Liu Y, Dong W, Tuo Q, Liu D, Wu F, Zhang L, Wu J, Zhang W. 2013. Impacts of Mycobacterium tuberculosis with different virulence on expressions of ferroportin and ferritin in infected macrophages. Chinese Journal of Cellular and Molecular Immunology, 29(7):710-713.

  14. 14) López M, Sly LM, Luu Y, Young D, Cooper H, Reiner NE. 2003. The 19-kDa Mycobacterium tuberculosis protein induces macrophage apoptosis through Toll-like receptor-2. Journal of Immunology, 170(5):2409-2416.

  15. 15) López-Rincón G, Mancilla R, Pereira-Suárez AL, Martínez-Neri PA, Ochoa-Zarzosa A, Muñoz-Valle JF, Estrada-Chávez C. 2015. Expression of autocrine prolactin and the short isoform of prolactin receptor are associated with inflammatory response and apoptosis in monocytes stimulated with Mycobacterium bovis proteins. Experimental and Molecular Pathology, 98:517-526.

  16. 16) Ludwiczek S, Aigner E, Theurl I, Weiss G. 2003. Cytokine-mediated regulation of iron transport in human monocytic cells. Blood, 101:4148-4154.

  17. 17) McKie A, Marciani P, Rolfs A, Brennan K, Wehr K, Barrow D, Miret S, Bomford A, Peters T, Farzaneh F, Hediger M, Hentze M, Simpson R. 2000. A novel duodenal iron-regulated transporter, IREG1, implicated in the basolateral transfer of iron to the circulation. Molecular Cell, 5:299-309.

  18. 18) Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, Ward DM, Ganz T, Kaplan J. 2004. Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science, 306:2090-2093.

  19. 19) Peyssonnaux C, Zinkernagal AS, Datta V, Lauth X, Johnson R, Nizet V. 2006. TLR4-dependent hepcidin expression by myeloid cells in response to bacterial pathogens. Blood, 107:3727-3732.

  20. 20) Qiagen. pcr Protocols & Applications [en línea]. https://www.qiagen.com/mx/re- sources/molecular-biology-methods/pcr/#Real-time [consulta: 03 mar 2015].

  21. 21) Sánchez A, Espinosa P, Esparza MA, Colon M, Bernal G, Mancilla R. 2008. Mycobacterium tuberculosis 38-kDa lipoprotein is apoptogenic for human monocyte- derived macrophages. Scandinavian Journal of Immunology, 69:20-28.

  22. 22) Van Zandt K, Sow F, Florence W, Zwilling B, Satoskar A, Schlesinger L, Lafuse W. 2008. The iron export protein ferroportin 1 is differentially expressed in mouse macrophage populations and is present in the mycobacterial-containing phagosome. Journal of Leukocyte Biology, 84:689-700.

  23. 23) Weiss G, Werner F, Werner E, Grunewald K, Wachter H, Hentze M. 1994. Iron regulates nitric oxide synthase activity by controlling nuclear transcription. Journal of Experimental Medicine, 180:969-976.

  24. 24) Weiss G. 2009. Iron metabolism in the anemia of chronic disease. Biochimica et Biophysica Acta, 1790:682-693.

  25. 25) Yang F, Liu X, Quinones M, Melby P, Ghio A, Haile D. 2002. Regulation of reticuloendothelial iron transporter MTP1 (Slc11a3) by inflammation. Journal of Biological Chemistry, 277:39786-39791.

  26. 26) Zhu X, Tu ZJ, Coussens PM, Kapur V, Janagama H, Naser S, Sreevatsan S. 2008. Transcriptional analysis of diverse strains Mycobacterium avium subspecies paratuberculosis in primary bovine monocyte derived macrophages. Microbes and Infection, 10:1274-1282.




2020     |     www.medigraphic.com