Rodríguez-Gutiérrez I, Domínguez-Rebolledo Á, Loeza-Concha H, Castellanos-Zacarías C, Tun-Moo M, Ramón-Ugalde J

Idioma: Español
Referencias bibliográficas: 22
Paginas: 1-9
Archivo PDF: 478.12 Kb.

RESUMEN

El objetivo del estudio fue evaluar el efecto de los antioxidantes Trolox y Crocina en el diluyente de congelación sobre la criopreservación del semen de ovino Pelibuey. Los eyaculados obtenidos se mezclaron, dividieron y congelaron en 3 tratamientos: T1: Trolox 1mM; T2: Crocina 1mM; TT: Testigo (sin antioxidante). Se analizó a las 0 horas (descongelación) y 6 horas a 37 ^°C, la motilidad total (MT) y progresiva (MP), la viabilidad espermática, la actividad mitocondrial, la integridad de los acrosomas y de la membrana de la cola (Host). Los datos se analizaron con un ANOVA para un diseño completamente al azar y una prueba de Tukey para la comparación de medias. En ninguna de las dos horas se encontraron diferencias significativas en los tratamientos (P›0.05). Sin embargo, de 0 a 6h, la MT se comportó de forma similar en el T1 y T2, pero no así en el TT (P‹0.01), por el contrario, la integridad de los acrosomas en los tres tratamientos fue similar (P›0.05). La MP, viabilidad, actividad mitocondrial y Host disminuyeron con el tiempo de forma similar en los tres tratamientos. Los antioxidantes Trolox y Crocina a 1mM en el diluyente de congelación, permiten mantener la MT y la integridad de los acrosomas durante 6 h de incubación a 37 ^°C.

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