2020, Number 1
Antioxidant effect of Trolox and Crocin on the cryopreservation of Pelibuey rams semen
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Language: Spanish
References: 22
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ABSTRACT
The aim of this study was to assess the effect of two antioxidants (Trolox and Crocin) added to the freezing extender on the cryopreservation of Pelibuey ram semen. Semen samples were pooled, split and frozen into 3 treatments: T1: Trolox 1mM; T2: Crocin 1mM; TT: Control (without antioxidant). Total motility (MT), progressive motility (PM), sperm viability, mitochondrial activity, acrosomes integrity, and plasma membrane integrity of the tail (Host) were assessed at 0 h (thawed), and at 6 hours at 37 °C. The results were analyzed with ANOVA, followed by Tukey’s comparison test. At 0 h and 6 h, no differences were found (P› 0.05) in all the parameters evaluated between treatments, however, from 0 to 6 hours, the MT was similar in the T1 and T2, but not in the TT (P ‹0.01), on the contrary, acrosome integrity in the three treatments was similar (P›0.05). MP, viability, mitochondrial activity, and Host dropped through time in a similar way in the three treatments. The addition of the antioxidants Trolox and Crocin at 1 mM to the freezing extender allows maintaining the total motility and acrosome integrity during 6 h of incubation at 37 °C.REFERENCES
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