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Instituto Nacional de Neurología y Neurocirugía

2021, Number 2

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Arch Neurocien 2021; 26 (2)

Glutamine-mediated nitric oxide synthase inhibition might explain the ‘arginine paradox’

Pérez-Neri I, Ríos-Castañeda C

Language: English
References: 18
Page: 7-9
PDF size: 192.09 Kb.


Text Extraction

From several studies, it has been described that nitric oxide synthase(NOS) activity increases if extracellular arginine levels are elevated despite of the high intracellular amino acid level (0.1 - 2 mM in endothelial cells 3 ), which would maintain the enzyme saturated. This ‘arginine paradox’ may be explained by several mechanisms including amino acid transport, translational control and the presence of endogenous NOS inhibitors.
Increasing extracellular arginine concentration (10-1000 µM), in a range including levels found in neurologic patients, favors an increased cytokine-activated inducible NOS (iNOS) activity in cultured astrocytes through a translational mechanism. Also, at the 100-µM extracellular level, arginine increases nitrite synthesis by endothelial cells.


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