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2020, Number 1

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Rev Cubana Farm 2020; 53 (1)

Evaluation of in vitro activity of antibacterial combinations against methicillin-resistant Staphylococcus aureus

Uribe MS, Durán LM, Caraballo MR

Language: Spanish
References: 18
Page: 1-16
PDF size: 541.30 Kb.


ABSTRACT

Introduction: Antibacterial resistance is a growing phenomenon characterized by the partial or total refractoriness of microorganisms to antibiotic effect, which causes ineffective treatments and increases the risk of spreading resistant pathogens.
Objective: To evaluate in vitro pharmacodynamic interaction between combinations of linezolid, vancomycin, daptomycin and tigecycline against clinical isolates of methicillinresistant Staphylococcus aureus from patients at a local hospital in Cartagena de Indias.
Methods: Methicillin-resistant Staphylococcus aureus isolates were recovered from sputum, blood, and urine samples from patients at the University Hospital of the Caribbean. The isolates were confronted to the combinations linezolid-vancomycin, linezolid-daptomycin, linezolid-tigecycline, vancomycin-tigecycline, vancomycin-daptomycin, and daptomycintigecycline. To evaluate the activity of these combinations, the minimum inhibitory concentration and the fractional inhibitory concentration were determined using a broth microdilution checkerboard method.
Results: The tigecycline-daptomycin combination was shown to present in vitro synergism in 93.8% of cases and was confronted to different clinical Staphylococcus aureus isolates. The vancomycin-tigecycline combination showed antagonism in 43.8% of the trials and indifference in 38%. Other combinations, such as vancomycin-daptomycin, linezoliddaptomycin, linezolid-vancomycin and linezolid-tigecycline, had a pharmacodynamic behavior in which indifference prevailed.
Conclusions: The tigecycline-daptomycin combination presents better in vitro antibacterial synergism against methicillin-resistant Staphylococcus aureus, which makes it a promising alternative against infections caused by this pathogen. The vancomycin-tigecycline combination shows pharmacological antagonism, so it should not be used in vivo.


REFERENCES

  1. Alos J. Resistencia bacteriana a los antibióticos: una crisis global Antibiotic resistance: A global crisis. Enfermedades Infecciosas y Microbiología Clínica. 2015;33(10):692-99.

  2. Espinosa C, Castillo JS, Cortés JA, Leal AL. Revisión sistemática de la resistencia antimicrobiana en cocos Gram positivos intrahospitalarios en Colombia. Biomédica. 2011;31:27-34.

  3. Yomayusa N, Álvarez CA, Hernández PA, Ibáñez M, Sossa MP, Suárez C, et al. Las infecciones por Staphylococcus aureus resistente a meticilina son un problema de salud pública. Revista Médica Sanitas. 2009;3:8-16.

  4. Organización Mundial de la Salud. Resistencia a los antimicrobianos. Ginebra: OMS; 2013 [acceso 19/08/2016]. Disponible en: http://www.who.int/mediacentre/factsheets/fs194/es/

  5. Sueke H, Kaye SB, Neal T, Hall A, Tuft S, Parry CM. An in vitro investigation of synergy or antagonism between antimicrobial combinations against isolates from bacterial keratitis. Invest Ophthalmol Vis Sci. 2010;51(8):4151-55. DOI:10.1167/iovs.09-4839

  6. American Type Culture Collection. Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 43300™). Manassas VA: ATCC; 2010 [acceso 18/08/2016]. Disponible en: https://www.atcc.org/products/all/43300.aspx#documentation

  7. Clinical and Laboratory Standards Institute (CLSI). M100. Performance Standards For Antimicrobial Suceptibility Testing; Twenty-Fourt Informational Supplement. 2015;25:1- 240.

  8. Zhurbenko R, Rodríguez Martínez C, Díaz Pérez M, Durán Vila A, López Hernández O, Viera Oramas D. Caracterización de la peptona de soya para el cultivo de microorganismos. Revista Cubana de Medicina Tropical. 2006 [acceso 03/07/2020];58(2):109-118. Disponible en: https://imbiomed.com.mx/1/1/articulos.php?method=showDetail&id_articulo=38892&id_s eccion=595&id_ejemplar=4013&id_revista=71

  9. Carret G, Cavallo J, Chardon H, Chidiac C, Choutet P, Courvalin P, et al. 2001. Comite De L’antibiogramme De La Société Francaise De Microbiologie Report 2000-2001. Francia: Centre Hospitalier Universitaire Henri Mondor; 2015 [acceso 17/08/2016]. Disponible en: http://www.sfm-microbiologie.org/UserFiles/files/casfm_2002english.pdf.

  10. Madhuri M. Sopirala. Synergy Testing by Etest, Microdilution Checkerboard, and Time- Kill Methods for Pan-Drug-Resistant Acinetobacter baumannii. Antimicrobial Agents and Chemotherapy. 2010;54(11):4678-4683.

  11. Picazo J, Betriu C, Rodrıguez-Avial I, Culebras E, López F, Gómez M, Grupo VIRA. Actividad comparativa de la daptomicina frente a Staphylococcus aureus resistente a meticilina y frente a estafilococos coagulasa negativa. Enfermedades Infecciosas y Microbiología Clínica. 2010;28(1):13-16.

  12. Araos R, Garcia P, Chanqueo L, Labarca J. Daptomicina: características farmacológicas y aporte en el tratamiento de infecciones por cocáceas gram positivas. Revista chilena de infectología. 2012;29(2), 127-31.

  13. Mensa J, Soriano A, Llinares P, Barberán J, Montejo M, Salavert M, et al. Guía de tratamiento antimicrobiano de la infección por Staphylococcus aureus. Revista Española de Quimioterapia. 2013;26 (Supl 1):1-84.

  14. Aguadero V, González-Velasco C, Vindel A, González-Velasco Moreno J. Situación actual de la infección por Staphylococcus aureus resistente a meticilina en Extremadura: sensibilidad, clonalidad, y protagonismo de la adquisición extrahospitalaria. Revista Española de Quimioterapia. 2014;27(3):180-89.

  15. Kelesidis T, Humphries R, Ward K, Lewinski M, Yanga O. Combination therapy with daptomycin, linezolid, and rifampin as treatment option for MRSA meningitis and bacteremia. Diagnostic Microbiology and Infectious Disease. 2011;71:286-90.

  16. Steed M, Vidaillac C, Rybak M. (2010). Novel Daptomycin Combinations against Daptomycin-Nonsusceptible Methicillin-Resistant Staphylococcus aureus in an In Vitro Model of Simulated Endocardial Vegetations. Antimicrobial Agents and Chemotherapy. 2010;54(12):5187-92

  17. Entenza J, Moreillon P. Tigecycline in combination with other antimicrobials: a review of in vitro, animal and case report studies. International Journal of Antimicrobial Agents. 2009;34(1):8.e1-9. DOI: 10.1016/j.ijantimicag.2008.11.006

  18. Sader H, Fritsche T, Jones R. The evaluation of the bactericidal activity of daptomycin, vancomycin and linezolid and determination of the interactions of these antimicrobials with gentamicin or rifampin against S. aureus. (2009). EE. UU.: JMI Laboratories-North liberty, IA; 2016. [acceso 17/08/2016]. Disponible en: https://jmilabs.com/data/posters/ECCMID2007/706.PDF




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