Montalvo R, Vargas R, Ochoa S, Rojas A, Caballero K

Language: Spanish
References: 15
Page: 1-7
PDF size: 321.53 Kb.

ABSTRACT

Introduction: The skin surface is inhabited by microorganisms responsible for the biochemical balance; some are pathogenic and persist despite hand washing.
Objective: To identify, in university students, the bacterial flora resistant to hand washing.
Methods: A longitudinal, analytical and observational study was carried out, for which university students were included who previously received educational talks on hand hygiene. Samples were taken before and after hand washing.
Results: Of 80 samples analyzed, Staphylococcus epidermidis was identified as the most frequent in 95% of the students and, with hand washing, it was reduced to 60%. The second agent was Staphylococcus saprophyticcus, present in 75% of the cases and, after hand washing, in 35%. E. coli occupied the third position, as it was found in 42.5% of the samples and, after hand hygiene, it was reduced to 17.5%. For transient agents like Klebsiella (P<0.05) and Pseudomonas, handwashing was more effective; however, E. coli was the enterobacterium that remained elevated despite hand hygiene (P=0.01).
Conclusion: Some E. coli colonies are resistant to hand washing, similar to resident agents such as Staphylococcus epidermidis, Staphylococcus saprophyticcus and Staphylococcus aureus.

REFERENCES

1. Larson E. Skin hygiene and infection prevention: more of the same or different approaches? Clin Infect Dis. 1999;29:1287-94.

2. Boyce JM, Pittet D. Healthcare Infection Control Practices Advisory Committee; HICPAC/SHEA/APIC/IDSA Hand Hygiene Task Force. Guideline for Hand Hygiene in Health-Care Settings. Society for Healthcare Epidemiology of America/Association for Professionals in Infection Control/Infectious Diseases Society of America. MMWR Recomm Rep. 2002;51(RR-16):1-45.

3. Organización Mundial de la Salud. Guía de aplicación de la estrategia multimodal de la OMS para la mejora de la higiene de las manos. Ginebra- Suiza: OMS; 2009.

4. Londoño A, Murillas M. Eficacia de la higiene de manos con un preparado de base alcohólica vs lavado de manos con agua y jabón. Acta Med Colombiana. 2011:17(2):36-4.

5. Nantasit L, Hongsuwan M, Limmathurotsakul D, Lubell Y, Lee A, Harbarth S, et al. Comparative efficacy of interventions to promote hand hygiene in hospital: systematic review and network meta-analysis. BMJ. 2015;351:h3728.

6. Larson E, Hughes NCA, Pyrek JD, Sparks SM, Cagatay EU, Barkus JM. Changes in bacterial flora associated with skin damage on hands of health care personnel. Am J Infect Control. 1998;26:513-21.

7. Montalvo R, Huaroto L, Alvarezcano J, Ticona E, García Y. Prevalencia de portadores nasales por Staphylococcus aureus meticilino resistente en personal de salud del servicio de Cuidados intensivos, Hospital Nacional Dos de Mayo. Revista Peruana de Epidemiología. 2009[acceso: 22/08/2018]:08(4):08-13. Disponible en:

8. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A9. Wayne, PA: Clinical and Laboratory Standards Institute; 2016.

9. Schoch J, Monir K, Satcher D, Harris J, Triplett E, Neu J. The infantile cutaneous microbiome: A review First published. Pediatric Dermatology. 2019;36(5):67-71.

10. Asto M, Huamán D, Huamán R. Efectividad de un programa educativo en la Práctica de lavado de manos en familiares de la Unidad de cuidados intensivos del HNAL [tesis]. 2017 [acceso: 07/10/2018]. Disponible en: Disponible en: http://repositorio.upch.edu.pe/bitstream/handle/upch/1343/Efectividad_AstoHuaman_Mar%C3%ADa.pdf?sequence=1&isAllowed=y

11. Murray PR, Rosenthal KS, Pfaller MA. Microbiología Médica. 8va ed. Madrid, España: Elsevier; 2017.

12. Teker B, Ogutlu A, Gozdas HT, Ruayercan S, Hacialioglu G, Karabay O. Factors affecting hand hygiene adherence at a private hospital in Turkey. Eurasian Journal of Medicine. 2017;47(2):208-12.

13. Osman JR, Regeard C, Fernandes G, DuBow M. Variation of bacterial biodiversity from saline soils and estuary sediments present near the Mediterranean Sea coast of Camargue (France). Antonie Van Leeuwenhoek. 2019;112(3):351-65.

14. Scheithauer S, Batzer B, Dangel M, Passweg J, Widmer A. Workload even affects hand hygiene in a highly trained and well-staffed setting: A prospective observational study. Journal of Hospital Infection. 2017;97:11-16.

15. Okada J, Yamamizu Y, Kiyoko Fukai. Effectiveness of hand hygiene depends on the patient’s healthcondition and care environment. Japan Journal of Nursing Science. 2016;13(3):413-23.