Terán PEC, Cuéllar CA, Salas OE, Pardo AGL

Language: Spanish
References: 10
Page: 1-13
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ABSTRACT

Introduction: Croton wagneri Müll. Arg. is a plant species endemic to Ecuador used for medicinal purposes in ancestral Andean tradition.
Objective: Evaluate the acute toxicity of orally administered C. wagneri hydroalcoholic extract and its irritating effect on the oral mucosa.
Method: Acute toxicity of orally administered C. wagneri hydroalcoholic extract was evaluated in 24 female albino Wistar rats obtained from the Center for Laboratory Animal Breeding (CENPALAB) in compliance with standards issued by the Organization for Economic Co-operation and Development (OECD/OCDE 423). The animals were divided into four groups to receive oral administration of extracts from leaves, stems, inflorescences and the whole plant at doses of 5, 50, 300 and 2 000 mg/kg, respectively. After 14 days' observation, the animals were sacrificed for histopathological examination. Irritating action on the oral mucosa was evaluated in 30 Syrian golden hamsters. Seven days later the animals were sacrificed for evaluation. The statistical software GraphPad Prism 5 was used for biological evaluation.
Results: Extracts from the different parts of the plant were not found to cause any clinical sign of injury to the lungs, kidneys, heart, spleen or stomach, not even when administered orally at the maximum test dose of 2 000 mg/kg, nor did they have an irritating effect on the mucosa.
Conclusions: Changes were not observed in the behavior of albino Wistar rats or Syrian golden hamsters. It is thus concluded that no changes occurred in the clinical signs of the animals studied.

REFERENCES

1. Motto P. Arbustos. En: Aguirre Zhofre. Especies Forestales de los Bosques Secos del Ecuador. Ecuador: Ministerio del Ambiente Ecuador; 2012. p. 70-106.

2. Coy C, Gómez D. Castiblanco F. Importancia medicinal del género Croton (euphorbiaceae). Revista Cubana de Plantas Medicinales. 2016;21(2):234-47.

3. León S, Valencia R, Pitman N. Endara L, Ulloa C. Libro rojo de las plantas endémicas del Ecuador. Vol 1. 2th ed. Pontificia Universidad Católica del Ecuador; 2011.

4. Fretes F. Plantas medicinales y aromáticas una alternativa de producción comercial. Paraguay: Agencia del Gobierno de los Estados Unidos para el Desarrollo Internacional (USAID); 2010.

5. Tassou C. Drosinos, E. Nychas G. Inhibition of resident microbial flora and Pathogen inocula on cold Fresh fish fillets in olive oil, oregano, and lemon juice under modified atmosphere on air. Journal of Food Protection. 1995;59(1):31-4.

6. Aguirre Zhofre. Especies Forestales de los Bosques Secos del Ecuador. Ecuador: Ministerio del Ambiente Ecuador; 2012. p. 70-106.

7. AOAC. Official Methods of Analysis, 18th ed., Association of Official Analytical Chemists. Gaithersburg, MD; 2006.

8. ISO 10993. Parte 10 Biological evaluation of medical devices; 2009.

9. Verde soto D. Jaramillo C. Rubio O. Zaldúa J. García G. Rojas L. composición química, actividad cicatrizante y toxicidad del látex de Croton lechleri. FVC-LUZ. 2016;26(2):95-103.

10. Torrico F. Evaluación de la toxicidad aguda, actividad analgésica e hipoglicemiante del extracto acuoso de Croton pungens en animales experimentales. Scientific Journal from the Experimental Faculty of Sciences. 2013;21(4):181-91.