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2022, Number 1

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Sal Jal 2022; 9 (1)

Celulas troncales aisladas de piezas dentales provenientes de población mexicana el Estado de Jalisco

Sánchez-Gómez I, Gonzáles-Pelayo GL, Santibáñez-Escobar LP, Gaona-Bernal J, Villa-García Torres LS, Márquez-García E, Avelar-Rodríguez FJ, Marino-Marmolejo EN, Flores-Hernández FY

Language: Spanish
References: 35
Page: 16-27
PDF size: 482.39 Kb.


ABSTRACT

Research on stem cells regenerating damaged tissues has grown due to the potential to improve various ailments. Tooth stem cells are an option to be used in tissue engineering, requiring standardized procedures for their processing in order to achieve adequate isolation and culture. Objective: To carry out an evaluation of different procedures for the isolation, culture and propagation of dental pulp stem cells (DPSC). Materials and Methods: Quasi-experimental study with non-probabilistic sampling for isolation of DPSC, donated under informed consent. Various methods were tried; for access to the pulp chamber, mechanical drilling was tested using milling machines and high-speed handpieces, as well as a cutting method at the cervical third level dividing crown and root using a low-speed handpiece with a diamond disc. The dental pulp was extracted by disintegrating the cells by various enzymatic methods proceeding to monolayer culture for isolation. Cell phenotyping was by flow cytometry detecting mesenchymal markers (CD44, 73, 90, 105); in addition, multidifferentiation tests were carried out towards osteogenic, chondrogenic and adipogenic lineages. Finally, the DPSCs were cryopreserved in liquid nitrogen. Results: Efficient procedure for obtaining, isolating and proliferating DPSC, confirming with morphology, expression of mesenchymal markers, multipotentiality and plastic adhesion. Conclusion: A DPSC isolation protocol was standardized (transport, dental processing, isolation, culture and proliferation), observing better isolation of DPSC from molars with immature apex, from patients with an average age of 25 years.


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