2020, Number 2
Changes in redox status associated with polymorphisms of the glutathione S-transferase gene in women with a family history of breast cancer
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Language: Spanish
References: 20
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ABSTRACT
Introduction: The involvement of cellular redox status and antioxidant mechanisms is related to several of the risk factors for breast cancer.Objectives: To determine the association between oxidative stress markers and glutathione S-transferase gene polymorphisms in women with a family history of breast cancer.
Methods: A case control-study was carried out. A total of 81 females were included, 47 of them with family antecedents of I and II grade of breast cancer and 34 as controls. They were determined: plasma concentrations of malonildialdehyde, advanced products of protein oxidation, enzymatic activities of Cu-Zn superoxide dismutase, catalase and glutathione peroxidase by spectrophotometric methods. In addition, the presence of the homozygous deletion of the GSTM1 and GSTT1 genes was detected by multiplex PCR.
Results: The women with a family history of breast cancer showed an increase in the concentrations of malonildialdehyde together with a high activity of the glutathione peroxidase with respect to the control group. 53.1% and 12.5% of the women with a family history had the homozygous deletion of the GSTM1 and T1 genes, respectively. The combination of the functional variants M1+/T1+ was related to an increase in the enzyme antioxidant systems, while the combination M1*0/T1+ was associated with the increase in the concentrations of non-enzymatic antioxidants in the plasma.
Conclusions: Malonildialdehyde levels and Glutathione peroxidase activity could be considered risk factors in women with a family history. The combinations of the variants of the GST genes are related to the activation of compensatory antioxidant mechanisms.
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