medigraphic.com
ISSN 1561-2988 (Print)

2021, Number 2

<< Back Next >>

Rev Cubana Farm 2021; 54 (2)

Morphological, chemical characterization and anti-inflammatory activity of Tropaeolum tuberosum (Ruiz & Pav.) Kuntze (Tropaeolaceae) pink variety

Jiménez HME, Miranda MM, Chóez GIA, Gutiérrez GYI, Scull LR, Abreu PJ

Language: Spanish
References: 34
Page: 1-17
PDF size: 409.31 Kb.


ABSTRACT

Introduction: Tropaeolum tuberosum (Ruiz & Pav.) Kuntze. (Tropaeolaceae) has different varieties of tubers (types: black, yellow and pink), very used in Ecuador with nutritional aims and traditionally used due to their medicinal properties. However, there are few scientific studies on the pink variety.
Objective: Assess the morphological characteristics of the tubers, the chemical composition of the non-polar extracts and the anti-inflammatory activity of T. tuberosum pink variety.
Methods: There were assessed the macromorphological and micromorphological characteristics of T. tuberosum pink variety’s tubers. From the vegetal material, there were made extractions by maceration with hexane, ethyl acetate and ethanol at 30%. The extracts in hexane and ethyl acetate were analyzed by gas chromatography – mass spectrometry (CG-EM). It was assessed the antiinflammatory activity of the hydroalcoholic extract through the model of plantar edema induced by carrageenan in rats to the dose of 500mg/kg, using indometacin as positive control.
Results: Some differences were perceived in the macromorphological characteristics and micromorphological similarities in respect to other varieties of T. tuberosum. In the extract in hexane predominated the fatty acids (linoleic acid, mainly), while in the extract of ethyl acetate prevailed the hydrocarbons. Other compounds detected in both extracts were phytosterols and triterpenoids. The hydroalcoholic extract showed an anti-inflammatory activity comparable with the one of the indometacin in the fifth hour of the study, with a percentage of inhibition of the edema over the 50%.
Conclusions: The morphological characteristics of T. tuberosum pink variety are an efficient tool for its correct botanical identification. The bioactive compounds found in the tubers can be considered as responsible of the confirmed antiinflammatory activity.


REFERENCES

  1. Shen B. A New Golden Age of Natural Products Drug Discovery. Cell. 2015;163:1297- 1300. DOI: 10.1016/j.cell.2015.11.031

  2. Harvey A, Edrada ER, Quinn RJ. The re-emergence of natural products for drugdiscovery in the genomics era. Nat. Rev. Drug Discov. 2015;14:111-29. DOI:10.1038/nrd4510

  3. Newman DJ, Cragg GM. Natural Products as Sources of New Drugs from 1981 to2014. J. Nat. Prod. 2016;79:629-661. DOI: 10.1021/acs.jnatprod.5b01055

  4. Tena PV, Nestor ATL. Ethnobotanical uses, secondary metabolites andbiological activities of Mashua (Tropaeolum tuberosum Ruíz & Pavón), Journal ofEthnopharmacology 2020;247:112152. DOI: 10.1016/j.jep.2019.112152

  5. Patridge E, Gareiss P, Kinch MS, Hoyer D. An analysis of FDA-approved drugs:natural products and their derivatives. Drug Discov. Today. 2016; 21: 204-207.DOI: 10.1016/j.drudis.2015.01.009

  6. Bussmann RW, Sharon D. Plantas medicinales de los Andes y la Amazonia. LaFlora mágica y medicinal del Norte del Perú. Ethnobotany Research andApplications. 2018;15:1-293 [acceso 23/06/2021]. Disponible en:https://ethnobotanyjournal.org/index.php/era/article/view/1281

  7. Andersson L and Andersson S. Una filogenia molecular de Tropaeolaceae y susimplicaciones sistemáticas. Taxón. 2000;49(4):721-36. DOI: 10.2307/1223973

  8. Aruquipa R, Trigo RR, Bosque SH, Mercado RG, Condori MJ. El isaño(Tropaeolum tuberosum) un cultivo de consumo y medicina tradicional enHuatacana para el beneficio de la población boliviana. Revista De Investigación EInnovación Agropecuaria y de Recursos Naturales 2016 [acceso23/06/2021];3(2):33-8. Disponible en: http://riiarn.agro.umsa.bo/index.php/

  9. Johns T, Kitts WD, Newsome F, Towers GHN. Anti-reproductive and othermedicinal effects of Tropaeolum tuberosum. J. Ethnopharmacol. 1982;5:149-61.DOI: 10.1016/0378-8741(82) 90040-x

  10. Morán E, Budía A, Broseta E, Boronat F. Phytotherapy in urology. Currentscientific evidence of its application in benign prostatic hyperplasia and prostateadenocarcinoma. Actas Urol. Esp. 2013;37:114-19. DOI:10.1016/j.acuroe.2012.07.014

  11. Chirinos R, Campos D, Costa N, Arbizu C, Pedreschi R, Larondelle Y. Phenolicprofiles of andean mashua (Tropaeolum tuberosum Ruíz & Pavón) tubers:Identification by HPLC-DAD and evaluation of their antioxidant activity. FoodChemistry. 2008;106:1285-98. DOI: 10.1016 / j.foodchem.2007.07.024

  12. Jiménez HME, Gutiérrez GYI, Chóe GI, Miranda MM. Chemical Study andDetermination of the Antioxidant Activity of Three Varieties Tropaeolumtuberosum (Mashua). American Journal of Plant Sciences, 2019;10:2279-97. DOI:10.4236/ajps.2019.1012159D

  13. Jiménez HME, Gutiérrez GYI, Chóez GI, Miranda M. Pharmacognostic,Chemical and Anti-inflammatory Activity Study of Two Varieties of Tropaeolumtuberosum (Ruiz & Pav.) Kuntze (Tropaeolaceae). International Journal ofPharmacy and Chemistry 2020;6(4):41-53. DOI: 10.11648/j.ijpc.20200604.12

  14. Gattuso MA, Gattuso SJ. Manual de procedimientos para el análisis de drogasen polvo. Editorial de la Universidad Nacional de Rosario Urquiza. Argentina;1999.

  15. Miranda MM, Cuéllar AC. Manual de prácticas de laboratorio. Farmacognosia yproductos naturales. Universidad de La Habana. Ciudad Habana. Cuba. 2000:1-7:18-32.

  16. González GMC, Ospina GLF, Rincón VJ. Actividad antiinflamatoria deextractos y fracciones de Myrcianthes leucoxila, Calea prunifolia, Curatellaamericana y Physalis peruviana en los modelos edema auricular por TPA, edemaplantar por carragenina y artritis inducida por colágeno. Biosalud. 2011;10(1):9-18.

  17. Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw ofthe rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol. 1962;111:544-7. DOI: 10.3181/00379727-111-27849

  18. Balamurugan K, Sakthidevi G and Mohan VR. Antiinflammatory activity of leafof Melastoma malabathricum L. (Melastomataceae). International Journal ofResearch in Ayurveda and Pharmacy. 2012;3(6):801-02. DOI: 10.7897/2277-4343.03622

  19. Diehl KH, Hull R, Morton D, Pfister R, Rabemampianina Y, Smith D, et al. AGood Practice Guide to the Administration of Substances and Removal of Blood,Including Routes and Volumes. J. Appl. Toxicol. 2001;21:15-23. DOI:10.1002/jat.727

  20. Declaración de la AMM sobre el Uso de Animales en la InvestigaciónBiomédica. Buenos Aires, Argentina; 2016 [acceso 02/03/2017]. Disponible en:https://www.wma.net/es/policies-post/declaracionde-la-amm-sobre-el-uso-deanimales-en-la-investigacionbiomedica/

  21. Khandelwal KR. Practical Pharmacognosy. Techniques and experiments.Twelveth edition. India: Nirali Prakashan; 2004 p. 138.19.

  22. Esau K. Anatomía vegetal. Edición Revolucionaria. Cuba. 1972: 355-410.

  23. Kor NM, Ezati P, Akbari M, Obeidavi Z, Nazer MR., Aghajanopour M.Functional foods and their role in cancer prevention and health promotion: acomprehensive review. Am. J. Cancer. Res. 2017;7:740-69.

  24. Ramallo ZR. Análisis exploratorio de los ácidos grasos del isaño (Tropaeolumtuberosum). Rev. Investigación & Desarrollo. 2004;4:71-7.

  25. Chasquibol SN, Delmás RDI, Rivera CD, Lengua CRL, Aguirre MR, Bazán GD, etal. Contribución a la normalización de productos tradicionales andinos: maca,Kiwicha, Cañihua, Mashua. Rev. Per. Quim. Ing. Quim. 1999;2(1):9-21.

  26. Almagro L, Miras MB, Sabater JAB. Bioactivity of phytosterols and theirproduction in plant in vitro cultures. J. Agric. Food Chem. 2016;38:7049-58. DOI:10.1021/acs.jafc.6b02345

  27. de-Almeida SCX, da-Silva ÂCF, Sousa NRT, Amorim IHF, Leite BG, Neves KRT,et al. Antinociceptive and anti-inflammatory activities of a triterpene-richfraction from Himatanthus drasticus. Brazilian Journal of Medical and BiologicalResearch 2019;52(5):e7798. DOI: 10.1590/1414-431X20197798

  28. Valenzuela A, Ronco AM. Phytosterols and phytostanols: Natural allied for theprotection of cardiovascular health. Rev Chil Nutr. 2004;21(1):161-69.

  29. Balamurugan R, Duraipandiyan V, Ignacimuthu S. Antidiabetic activity of γ-sitosterol isolated from Lippia nodiflora L. in streptozotocin induced diabeticrats. Eur J Pharmacol. 2011;667(1-3):410-18. DOI: 10.1016/j.ejphar.2011.05.025

  30. Ahmad TB, Rudd D, Kotiw M, Liu L and Benkendorff K. Correlation betweenfatty acid profile and anti-Inflammatory activity in Common Australian Seafoodby-Products. Marine drugs. 2019;17(155): 20. DOI: 10.3390/md17030155

  31. 31.Vasudevan A, Vijayan D, Mandal P. Anti-Inflammatory Property of n-Hexadecanoic Acid: Structural Evidence and Kinetic Assessment. ChemicalBiology & Drug Design. 2012;80(3):434-9.

  32. Awad SE, Hassan AM and Elazhari GEM. Fatty acid composition, antiinflammatoryand analgesic activities of Hibiscus sabdariffa Linn. Seeds. J. Adv.Vet. Anim. Res. 2014;1(2):50-7.

  33. Dirosa M and Giround J. Studies of the mediators of acute inflammatoryresponse induced in rats in different sites by carrageenan and turpentine. J.Pathol, 1971:15-29. DOI: 10.1002 / ruta.1711040103

  34. Apaza TL, Tena PV, Serban AM, Alonso NMJ, Rumbero A. Alkamides fromTropaeolum tuberosum inhibit inflammatory response induced by TNF-α and NF-κB. J Ethnopharmacol. 2019;0(235):199-205. DOI: 10.1016/j.jep.2019.02.015




2020     |     www.medigraphic.com