medigraphic.com
Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2023, Number 1

<< Back Next >>

Rev Biomed 2023; 34 (1)

Protein profile of Leishmania mexicana isolates with different virulence

Fernández FEA, Muñoz MSA, Ríos-Muñoz CA, Becker I, Rangel EC

Language: Spanish
References: 14
Page: 22-31
PDF size: 483.98 Kb.


ABSTRACT

Introduction. L. mexicana can cause two clinical forms of cutaneous leishmaniasis: localized (LCL) and diffuse (DLC). Gene expression in trypanosomatids is highly regulated at the post-transcriptional level, a massive and systematic analysis of proteins is a good approach to understand better those mechanisms that parasites have developed for increase survival and to become more pathogenic in the human host.
Objective. To analyze the protein profile of L. mexicana isolates that produce LCL and DCL.
Material and methods. Total protein extracts from 10 isolates of promastigotes in stationary phase were used and processed in a Q-Exactive plus mass spectrometer, bioinformatic analysis was performed.
Results. 779 proteins were identified, of which 57 were differentially expressed and were used for enrichment analysis, representing the pathways of aminoacylase activity, hydrolase activity, those associated with binding to cofactors, and chaperonins.
Conclusion. The proteins, FKBP and HSP60, can be studied for their clinical potential are due to the importance for parasite survival.


REFERENCES

  1. Fernández-Figueroa, EA. Análisis de Genes yProteínas de Células NK y Monocitos de Pacientes conLeishmaniasis Cutánea Localizada y Difusa. Facultad deMedicina. Universidad Nacional Autónoma de México.Mayo 2013.

  2. Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, CanoJ, et al. Leishmaniasis worldwide and global estimates ofits incidence. PloS One, 2010,7(5):e35671. doi: 10.1371/journal.pone.0035671.

  3. Berrueta TU. Leishmaniosis o leishmaniasis.Departamento de microbiología y parasitología. Facultadde Medicina, 2015. UNAM.

  4. Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA,Depledge DP, et al. Chromosome and gene copy numbervariation allow major structural change bet- weenspecies and strains of leishmania. Genome research,2012, 21(12):2129–2142. doi:10.1101/gr.122945.111.

  5. Seidelin H. Leishmaniasis and babesiasis in Yucatan.Annals of Tropical Medicine & Parasitology, 1912,

  6. 6(2):295–300. https://doi.org/10.1080/00034983.1912.11687069.6. Ivens AC, Peacock CS, Worthey EA, Murphy L,Aggarwal G, Berriman M, et al. The genome of thekinetoplastid parasite, Leishmania major. Science, 2015,309(5733):436–442. doi: 10.1126/science.1112680.

  7. Myler PJ, Fasel N. Leishmania: after the genome.Seattle, USA. Caiser Academic Press; 2008. https://doi.org/10.21775/9781912530304

  8. Paape D, Aebischer T. Contribution of proteomics ofLeishmania spp. to the understanding of differentiation,drug resistance mechanisms, vaccine and drugdevelopment. Journal of Proteomics, 2012, 74(9):1614–1624. https://doi.org/10.1016/j.jprot.2011.05.005

  9. Alcolea PJ, Alonso Larraga. Proteome profilingof Leishmania infantum promastigotes. Journal ofEukaryotic Microbiology, 2011, 58(4):352–358. doi:

  10. 10.1111/j.1550-7408.2011.00549.x10. Pawar H, Sahasrabuddhe NA, Renuse S, KeerthikumarS, Sharma J, Kumar G, et al. A proteogenomic approachto map the proteome of an unsequenced pathogen–Leishmania donovani. Proteomics, 2012, 12(6):832–844. doi: 10.1002/pmic.201100505.

  11. Biyani N, Singh AK, Mandal S, Chawla B, Madhubala R.Differential expression of proteins in antimony-susceptibleand resistant isolates of Leishmania donovani. Molecularand Biochemical Parasitology, 2011,179(2):91–99. doi:10.1016/j.molbiopara.2011.06.004

  12. Sardar AH, Kumar S, Kumar A, Purkait B, Das S, SenA, et al. Proteome changes associated with Leishmaniadonovani promastigote adaptation to oxidative andnitrosative stresses. Journal of Proteomics, 2013,81:185–199. doi: 10.1016/j.jprot.2013.01.011.

  13. Tsigankov P, Gherardini P F, Helmer-Citterich M,Späth G F, Zilberstein D. Phosphoproteomic analysisof differentiating Leishmania parasites reveals aunique stage-specific phosphorylation motif. Journalof Proteome Research, 2013, 12(7):3405–3412. doi:10.1021/pr4002492.

  14. Brotherton MC, Racine G, Ouameur AA, LeprohonP, Papadopoulou B, Ouellette M. Analysis ofmembrane-enriched and high molecular weightproteins in Leishmania infantum promastigotes andaxenic amastigotes. Journal of Proteome Research,2012,11(8):3974–3985. doi: 10.1021/pr201248h.




2020     |     www.medigraphic.com