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2022, Number 2

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VacciMonitor 2022; 31 (2)

Standardization of a spectrophotometric procedure for the quantification of Neisseria meningitidis serogroup X capsular polysaccharide

Cardoso-San Jorge F, Baró-Vinent B, Hernández-Hernández M, Pedroso-Fernández J, Veréz-Bencomo V

Language: Spanish
References: 18
Page: 83-89
PDF size: 320.30 Kb.


ABSTRACT

The present work comprises the standardization a spectrophotometric procedure for assessing Neisseria meningitidis, serogroup X capsular polysaccharide and their intermediates of modification, the phosphodiesters groups present in its structure, based on Chen method. An analysis of the following standardization criteria was performed: linearity, precision (repeatability and intermediate precision) and accuracy. It was demonstrated through the experimental design and the statistical procedures used that the method is linear (r > 0.99), the coefficient of variation of the response factor < 5%, the relative standard deviation of the slope < 2%, with no statistically significant difference between the intercept of the equation with respect to zero; exact, because there is no statistically significant difference between the concentration determined in a work material and its nominal concentration; it also proved to be repeatable, because the coefficient of variation of the concentrations of the sample (2.44; 2.43; 0.88 % for low, medium and high concentrations respectively) is less than 3% and there is no statistically significant difference between the means of the results obtained by two analysts, evaluated for four days at three concentration levels. Its intermediate precision was satisfactory.


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