2006, Number 3-4
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ABSTRACTThis work was conducted to identify indigenous LAB capable of antimicrobial activity, present in traditional Mexican-foods with potential as natural preservatives. A total of 27 artisan unlabeled Mexican products were evaluated, from which 94 LAB strains were isolated, and only 25 strains showed antimicrobial activity against at least one pathogen indicator microorganism. Most of the inhibitory activity showed by the isolated LAB strains was attributed to pH reduction by organic acids. Lactobacillus and Lactococcus strains were good acid producers, depending on the substrate, and may enhance the safety of food products. Cell free cultures of Leuconostoc mesenteroides CH210, and PT8 (from chorizo and pulque, respectively) reduced the number of viable cells of enteropathogenic E. coli in broth system. Lb. plantarum CC10 (from “madre” of vinegar) showed significant inhibitory effect against S. aureus 8943. E. faecium QPI1 (from panela cheese) produced a bacteriocin with wide anti-L. monocytogenes activity. Selected LAB from traditional Mexican foods showed good potential as bio-preservatives.
Ammor, S., G. Tauveron, E. Dufour & I. Chevallier. 2006. Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility. I-screening and characterization of the antibacterial compounds. Food Control 17:454-461.
Ananou, S., M. Garriga, M. Hugas, M. Maqueda, M. Martínez-Bueno, A. Galvez & E. Valdivia. 2005. Control of Listeria monocytogenes in model sausage by enterocin AS-48. Int. J. Food Microbiol. 103:179-190.
Andrighetto, C., E. Knijff, A. Lombardi, S. Torriani, M. Vancanneyt, K. Kersters, J. Swings & F. Dellaglio. 2001. Phenotypic and genetic diversity of enterococci isolated from Italian cheeses. J. Dairy Res. 68:303-316.
Arvizu-Medrano, S., M. H. Iturriaga & E. F. Escartín. 2001. Indicator and pathogenic bacteria in guacamole and their behavior in avocado pulp. J. Food Safety 21:233-244.
Bude, B. B., T. Hornabaek, T. Jacobsen, V. Barkholt & A. G. Koch. 2003. Leuconostoc carnosum 4010 as the potential for use as a protective culture for vacuum-package meats: Culture isolation, bacteriocin identification and meat application experiments. Int. J. Food Microbiol. 83:171-184.
Bulut, C., H. Gunes, B. Okuklu, S. Harsa, S. Kilic, H. S. Coban, & A. F. Yenidunya. 2005. Homofermentative lactic acid bacteria of a traditional cheese, Comlek peyniri from Cappadocia region. J. Dairy Res. 72:19-24.
Callon, C., L. Millet & M. Montel. 2004. Diversity of lactic acid bacteria isolated from AOC Salers cheese. J. Dairy Res. 71:231-244.
Calderon, M., G. Loiseau & J. P. Guyot. 2003. Fermentation by Lactobacillus fermentum Ogi E1 of different combinations of carbohydrates occurring naturally in cereals: consequences on growth energetics and a-amylase production. Int. J. Food Microbiol. 80:161-169.
Cintas, L. M., P. Casaus, H. Holo, P. E. Hernández, I. F. Ness & L. S. Håvarstein. 1998. Enterocins L50A and L50B, two novel bacteriocins from Enterococcus faecium L50, are related to staphylococcal hemolysin. J. Bacteriol. 180:1988-1994.
Cogan, T. M., M. Barbosa, E. Beuvier, B. Bianchi-Salvadori, P. S. Cocconcelli, I. Fernandez, J. Gomez, R. Gomez, G. Calantzopoulos, A. Ledda, M. Medina, M. Rea & E. Rodríguez 1997. Characterization of the lactic acid bacteria in artisanal dairy products. J. Dairy Res. 64:409-421.
Dellaglio, F., L. M. T. Dicks & S. Torriani. 1995. The genus Leuconostoc, pp. 235-278. In: B.J.B. Wood & W.H. Holzapfel (Eds.). The Genera of Lactic Acid Bacteria. Blackie Academic and Professional, London.
Devlieghere, F., L. Vermeiren & J. Debevere. 2004. New preservation technologies: Possibilities and limitations. Int. Dairy J. 14:273-285.
De Vuyst, L., M. R. Foulquié Moreno & H. Revets. 2003. Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins. Int. J. Food Microbiol. 84:299-318.
DeWaal, C. S., K. Barlow. 2002. Outbreak alert. CSPI. Whashington:34-37.
Escalante, A., M. E. Rodríguez, A. Martínez, A. López-Munguía, F. Bolívar & G. Gosset. 2004. Characterization of bacterial diversity in Pulque, a traditional Mexican alcoholic fermented beverage, as determined by 16S rDNA analysis. FEMS Microbiol. Lett. 235:273-279.
Escalante, A., C. Wacher & A. Farrés. 2001. Lactic acid bacterial diversity in the traditional Mexican fermented dough pozol as determined by 16S rDNA sequence analysis. International J. Food Microbiol. 64:21-31.
Foulquié Moreno, M. R., M. C. Rea, T. M. Cogan & L. De Vuyst. 2003. Aplicability of a bacteriocin-producing Enterococcus faecium as a co-culture in Cheddar cheese manufacture. International J. Food Microbiol. 81:73-84.
Harris, L. J., M. A. Daeschel, M. E. Stiles & T. R. Klaenhammer. 1989. Antimicrobial activity of lactic acid bacteria against Listeria monocytogenes. J. Food Protect. 52:384 387.
Herrera, T. 1993. Semblanza del estudio de las bebidas y de los alimentos fermentados mexicanos, pp. 21-27. In: M. C. Wacher & P. Lappe (Eds). Alimentos Fermentados Indígenas de México, UNAM, México.
Hoover, D. G. 2000. Microorganisms and their products in the preservation of foods, pp. 251-276. In: B. M. Lund, T. C. Baird-Parker & G. W. Gould (Eds). The Microbiological Safety and Quality of Food, Aspen Publishers, Maryland.
Leistner L. 1999. Combined methods for food preservation, pp. 457-485. In: M.S. Rahman (Ed.). Handbook of Food Preservation. Marcel Decker, New York.
Lewus, C. B. & T. J. Montville. 1991. Detection of bacteriocins produced by lactic acid bacteria. J. Microbiol. Meth. 13:145 150.
López-Díaz, T. M., C. Alonso, C. Román, M. L. García-López & B. Moreno. 2000. Lactic acid bacteria isolated from a hand-made blue cheese. Food Microbiol. 17:23-32.
Morea, M., F. Baruzzi & P. S. Cocconcelli. 1999. Molecular and physiological characterization of dominant bacterial populations in traditional Mozzarella cheese processing. J. Appl. Microbiol. 87:574-582.
Niness, K. R. 1999. Inulin and oligofructose: what are they? J Nutr. 129:1402S-1406S.
Noonpakdee, W., C. Santivarangakna, P. Jumriangrit, K. Sonomoto & S. Pangym. 2003. Isolation of nisin-producing Lactococcus lactis WNC 20 strain from nham, a traditional Thai fermented sausage. Int. J. Food Microbiol. 81:137-145.
Pu, Z. Y., M. Dobos, G. K. Y. Limsowtin & I. B. Powell. 2002. Integrated polymerase chain reaction-based procedures for the detection and identification of species and subspecies of the Gram-positive bacterial genus Lactococcus. J. Appl. Microbiol. 93:353-361.
Rodriguez, E., J. L. Arqués, P. Gaya, M. Nuñez & M. Medina. 2001. Control of Listeria monocytogenes by bacteriocins and monitoring of bacteriocin-producing lactic acid bacteria by colony hybridization in semi-hard raw milk cheese. J. Dairy Res. 68:131-137.
Rodríguez, J. M., M. I. Martínez, A. M. Suárez, J. M. Martínez & P. E. Hernández. 1997. Unsuitability of the MRS medium for the screening of hydrogen peroxide-producing lactic acid bacteria. Lett. Appl. Microbiol. 25:73-74.
Saavedra, L., M. P. Taranto, F. Sesma & G. F. deValdez. 2003. Homemade traditional cheeses for the isolation of probiotic Enterococcus faecium strains. Int. J. Food Microbiol. 88:241-245.
Schägger, H., G. Von Jagow. 1987. Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
Schillinger, U., & F. K. Lücke. 1987. Identification of lactobacilli from meat and meat products. Food Microbiol. 4:199 208.
Þimþek, O., A. H. Çon & S. Tulumoðlu. 2006. Isolating lactic starter cultures with antimicrobial activity for sourdough processes. Food Control 17:263-270.
Suzzi, G., M. Caruso, F. Gardini, A. Lombardi, L. Vannini, M. E Guerzoni, C. Andrighetto & M. T. Lanorte. 2000. A survey of the enterococci isolated from an artisanal Italian goat’s cheese (semicotto caprino). J. Appl. Microbiol. 89:267-274.
Villegas, A. 2004. Tecnología Quesera (pp. 242-268, 283, 338). Trillas, México.