medigraphic.com
ISSN 1561-3011 (Electronic)

2021, Number S1

<< Back Next >>

Rev Cubana Invest Bioméd 2021; 40 (S1)

MASP-2 saturation curve in cerebrospinal fluid indicates local synthesis and its equilibrium with soluble and associated forms

Dorta-Contreras AJ, González-Losada C, Ramos RA, Zerr I, Schmitz M, Gudmann-Hansen A

Language: English
References: 29
Page:
PDF size: 468.07 Kb.


ABSTRACT

Introduction: MASP-2 is a mannose blinding lectin associate to serine protease in cerebrospinal fluid and its dynamics through the blood brain barrier is unknown.
Objective: To describe MASP-2 diffusion pattern from blood to cerebrospinal fluid. Methods: A transversal observational prospective study was performed 56 control samples of cerebrospinal fluid and serum were employed. ELISA measured MASP-2. Two groups were made: control patients without organic brain disease with normal cerebrospinal fluid and normal barrier function and patients without inflammatory diseases with a blood cerebrospinal fluid barrier dysfunction.
Results: MASP-2 concentration in cerebrospinal fluid increase with augment the Q Albumin. QMASP-2 vs. Q Albumin saturation curve indicates that MASP-2 is interacting with other molecules in the subarachnoid environment. The higher inter-individual variation of cerebrospinal fluid MASP-2 of the control compared to the serum MASP-2 indicates that MASP-2 is a protein derived from blood.
Conclusions: MASP-2 in CSF is predominantly blood-derived. The saturation curve demonstrates that MASP-2 interacts with the starters of the lectin pathway like mannose binding lectin, ficolins and collectin LK.


REFERENCES

  1. Kjaer TR, Thiel S, Andersen GR. Toward a structure-based comprehension of the lectin pathway of complement. Mol. Immunol. 2013 [acceso 20/01/2021];56:413-22. Available in: https://pubmed.ncbi.nlm.nih.gov/23810291/1.

  2. Ma YJ, Skjoedt M, Garred P. Collectin-11/MASP Complex Formation Triggers Activation of the Lectin Complement Pathway - The Fifth Lectin Pathway Initiation Complex. J Innate Immun. 2013 [acceso 20/01/2021];5:242-50. Available in: https://pubmed.ncbi.nlm.nih.gov/23220946/2.

  3. Degn SE, Jensenius JC, Thiel S. Disease-Causing Mutations in Genes of the Complement System. Am J Hum Genet. 2011 [acceso 20/01/2021];88:689-705. Available in: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113252/3.

  4. Dorta-Contreras AJ, Padilla-Docal B, Pérez-Martín O, Arias-Morales A, Reiber H, Jensenius JC. MASP2 and MBL: Dynamics and Intrathecal synthesis. Front Immunol. 2013 DOI: https://doi.org/10.3389/conf.fimmu.2013.02.00929 Available in: http://dspace.uces.edu.ar:8180/dspace/handle/123456789/2004.

  5. Dorta-Contreras AJ, Lewczuc P, Noris-García E, Interián-Morales MT, Magraner Tarrau ME, Padilla-Docal B, et al. sICAM-1 in meningoencephalitis due to Angiostrongylus cantonensis. Arq Neuropsiquiatr. 2006;64:589-59. DOI: https://doi.org/10.1590/S0004-282X2006000400011

  6. Dorta-Contreras AJ, Reiber H. Teoría de la difusión molecular/flujo del líquido cefalorraquídeo. Rev Neurol. 2004 [acceso 20/01/2021];39:564-9. Available in: https://www.researchgate.net/publication/304640243_Teoría_de_la_difusion_molecularflujo_del_liquido_cefalorraquideo6.

  7. Reiber H. Cerebrospinal fluid data compilation and knowledge-based interpretation of bacterial, viral, parasitic, oncological, chronic inflammatory and demyelinating diseases. Diagnostic patterns not to be missed in neurology and psychiatry. Arq Neuropsiquiatr. 2016 [acceso 20/12/2020];74:337-50. Available in: https://pubmed.ncbi.nlm.nih.gov/27097008/7.

  8. Reiber H, Peter JB. Cerebrospinal fluid analysis: disease-related data patterns and evaluation programs. J Neurol Sci. 2001 [acceso 20/12/2020];184:101-22. Available in: https://pubmed.ncbi.nlm.nih.gov/11239944/8.

  9. Reiber H. Knowledge-base for interpretation of cerebrospinal fluid data patterns. Essentials in neurology and psychiatry. Arq Neuropsiquiatr. 2016 [acceso 20/12/2020];74:501-12. Available in: https://pubmed.ncbi.nlm.nih.gov/27332077/9.

  10. Dorta-Contreras AJ. Reibergramas: elemento esencial en el análisis inmunológico del líquido cefalorraquídeo. Rev Neurol. 1999 [acceso 20/12/2020];28:996-8. Available in: https://www.neurologia.com/articulo/9901910.

  11. Reiber H. Proteins in cerebrospinal fluid and blood: Barriers, CSF flow rate and source-related dynamics. Rest Neurol Neurosc. 2003 [acceso 20/12/2020];1:79-96. Available in: https://pubmed.ncbi.nlm.nih.gov/14530572/11.

  12. Reiber H. Dynamics of brain-derived proteins in cerebrospinal fluid. Clin Chim Acta. 2001 [acceso 20/12/2020];310:173-86. Available in: https://pubmed.ncbi.nlm.nih.gov/11498083/12.

  13. Reiber H, Padilla-Docal B, Jensenius JC, Dorta-Contreras AJ. Mannan binding lectin in cerebrospinal fluid: a leptomeningeal protein. Fluids. Barriers of the CNS. 2012;9(1):17. [acceso 20/12/2020]. Available in: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487976/13.

  14. Wildemann B, Oschmann P, Reiber H. Laboratory diagnosis in Neurology. Hansotto. 2010;292. Thieme Medical Publishers; Available in: https://www.thieme.com/books-main/neurology/product/1162-laboratory-diagnosis-in-neurology14.

  15. Ramos-Robledo A, Dorta-Contreras AJ, González Losada C, Zerr I, Schmitz M, Gudmann-Hansen A. Cocientes de CSF/suero para albúmina y MASP-2 con CSF individual y concentraciones séricas de MASP-2 en controles y pacientes con disfunciones de barrera y el CSF MASP-2 y CSF MASP-2 esperado individualmente, Mendeley Data, V1, 2020. DOI: http://dx.doi.org/10.17632/svj94tcp4n.1

  16. Frauenknecht V, Thiel S, Storm L, Meier N, Arnold M, Schmid JP, et al. Plasma levels of mannan-binding lectin (MBL)-associated serine proteases (MASPs) and MBL-associated protein in cardio and cerebrovascular diseases. Clin Exptal Immunol. 2013 [acceso 20/12/2020];73:112-20. Available in: https://pubmed.ncbi.nlm.nih.gov/23607747/16.

  17. Padilla Docal B, Dorta Contreras AJ, Reiber H, Iglesias González I, Jensenius JC, González Losada C. Neuroinmunología de la meningoencefalitis eosinofílica por Angiostrongylus cantonensis. In Robles L, Dorta Contreras AJ (eds.). Angiostrongylus cantonensis. Emergencia en América. La Habana: Academia; 2016. pp. 18-56.

  18. Dorta-Contreras AJ, Noris-García E, Padilla-Docal B, Rodríguez-Rey A, Bu-Coifiu-Fanego R, Magraner-Tarrau ME, et al. Reibergrama para la evaluación de la síntesis intratecal de c3c. Arq Neuropsiquiatr. 2006;64:585-8. DOI: https://doi.org/10.1590/S0004-282X2006000400010

  19. Padilla-Docal B, Dorta-Contreras AJ, Bu-Coifiu-Fanego R, Rodríguez-Rey A, Gutiérrez-Hernández JC, de Paula-Almeida SO. Reibergram of Intrathecal Synthesis of C4 in Patients with Eosinophilic Meningitis Caused by Angiostrongylus cantonensis. Am J Trop Med Hyg. 2010 [acceso 20/12/2020];82(6):1094-8. Available in: https://pubmed.ncbi.nlm.nih.gov/20519605/19.

  20. Sinha VK, Sharma OP, Kumar MS. Insight into the Intermolecular Recognition Mechanism Involved in Complement Component 4 Activation through Serine Protease-Trypsin. J Biomol Struc Dyn. 2017 [acceso 20/12/2020];29(2017):1-38. Available in: https://pubmed.ncbi.nlm.nih.gov/28132614/20.

  21. Bold ABT, Beltrame MH, Catarino SJ, Meissner CG, Tizzot R, Messias-Reason IJ. A dual role for Mannan-binding lectin-associated serine protease2 (MASP-2) in HIV infection. Mol Immunol. 2016 [acceso 21/12/2020];78:48-56. Available in: https://pubmed.ncbi.nlm.nih.gov/27588826/21.

  22. Reiber H, Flow rate of cerebrospinal fluid (CSF): a concept common to normal blood-CSF barrier function and to dysfunction in neurological diseases. J Neurol Sci. 1994 [acceso 21/12/2020];122:189-203. Available in: https://pubmed.ncbi.nlm.nih.gov/8021703/22.

  23. Bakota L, Ussif A, Jeserich G, Brandt R. Systemic and network functions of the microtubule-associated protein tau: implications for tau-based therapies. Mol Cell Neurosci. 2017 [acceso 21/12/2020];84:132-41. Available in: https://pubmed.ncbi.nlm.nih.gov/28318914/23.

  24. Anderson BJ, Reilly JP, Shashaty MG, Palakshappa JA, Wysoczanski A, Dunn TG, et al. Admission plasma levels of the neuronal injury marker neuronspecific enolase are associated with mortality and delirium in sepsis. J Crit Care. 2016 [acceso 18/12/2020]36:18-23. Available in: https://pubmed.ncbi.nlm.nih.gov/27546742/24.

  25. Reiber H. Flow rate of cerebrospinal fluid (CSF) - a concept common to normal blood-CSF barrier function and to dysfunction in neurological diseases. J Neurol Sci. 1994 [acceso 21/12/2020];122(2):189-203. Available in: https://pubmed.ncbi.nlm.nih.gov/8021703/25.

  26. Hansen SWK, Ohtanib K, Roy R, Wakamiya N. The collectins CL-L1, CLK1and CL-P1, and their roles in complement and innate immunity. Immunobiology. [acceso 21/12/2020]. Available in: https://pubmed.ncbi.nlm.nih.gov/27377710/

  27. Golshayan D, Wójtowicz A, Bibert S, Pyndiah N, Manuel O, Binet I, et al. Polymorphisms in the lectin pathway of complement activation influence the incidence of acute rejection and graft outcome after kidney transplantation. Kidney Int. 2016 [acceso 21/12/2020];89:927-38. Available in: https://pubmed.ncbi.nlm.nih.gov/26924055/27.

  28. Lewczuk P, Reiber H, Korenke GC, Bollensen E, Dorta-Contreras AJ. Intrathecal release of sICAM-1 into CSF in neuroborreliosis-increased brain derived fraction. J Neuroimmunology. 2000 [acceso 21/12/2020];103:93-6. Available in: https://www.sciencedirect.com/science/article/abs/pii/S016557289900221028.

  29. Lewczuk P, Reiber H, Tumani H. Intercellular adhesion molecule-1 in cerebrospinal fluid: the evaluation of blood-derived and brain-derived fractions in neurological diseases. J Neuroimmunol. 1998;87:156-61. DOI: https://doi.org/10.1016/S0165-5728(99)00221-0




2020     |     www.medigraphic.com