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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2026, Number 2

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Rev Biomed 2026; 37 (2)

Effect of Lactiplantibacillus plantarum in a model of formalin-induced inflammatory pain in rats

Estrada-Estrada AV, Guzmán-Priego CG, Baeza-Flores GC, García-Sánchez KE, Pacheco-Gil L

Language: Spanish
References: 30
Page: 47-53
PDF size: 257.61 Kb.


ABSTRACT

Introduction. Inflammatory pain is a type of adaptive and protective pain caused by tissue damage or infection and mediated by the release of inflammatory factors. Several studies have shown that probiotics, especially those of the Lactobacillus genus, can reduce inflammatory and nociceptive factors associated with pain relief.
Objective. To evaluate the antinociceptive effect of L. plantarum in a formalininduced inflammatory pain model.
Methods. Female Wistar rats weighing between 180 and 200 g were used. The animals were divided into two groups (n=4 each): a vehicle group without treatment and a probiotic group treated with L. plantarum (1.5 × 10⁸ CFU/ mL) orally for six months. At the end of the intervention, the formalin test was performed and the number of flinches of the injected limb was recorded over a 60-minute period. The area under the curve was then calculated, and the groups were compared using Student’s t-test.
Results. In phase 1, the group treated with L. plantarum showed a lower initial nociceptive response compared to the vehicle group, although this effect was not statistically significant. However, in phase 2, the group treated with the probiotic exhibited consistently fewer flinches over time, indicating an antinociceptive effect of L. plantarum.
Conclusions. These results support the hypothesis that L. plantarum could be a promising therapeutic alternative for managing acute inflammatory pain by acting through the gut-brain axis.


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