2007, Number 4
Homocysteine and mutation C677t and A1298C of the gene of 5-methyltetrahydrofolate reductase in pregnant women with preeclampsia-eclampsia
Briones VCG, García CR, Briones GJC
Full text
Language: Spanish
References: 5
Page: 179-184
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ABSTRACT
Introduction: Historically there is a relationship between high levels of homocysteine and arterial damage. The presence of hyperhomocysteinemia shows a dysfunction of the metabolic ways of this amino acid. The cellular export mechanism helps the going out of homocysteine from the inside of the cell to the plasma, avoiding its concentration inside the cell, preventing toxicity. The failure in the synthesis of N-5-Methyltetrahydrate due to an enzymatic defect of N-5-methyltetrahydrofolate reductase causes an increase of the synthesis of homocysteine.Objective: Demonstrate the presence of C6667T and A1298C mutations in the gene of the N-5-methyltetrahydrofolate reductase in patients with preeclampsia.
Material and methods: We analyzed comparatively a group of patients in the critical care unit with the diagnosis of preeclampsia (study group) and other group (control group) with normal pregnancy, randomly selected. We obtained DNA blood samples for its analysis with PCR-RFLP and southernblot.
The study was made between June 1st 2001 and May 30th 2002 in IMIEM Toluca, Estado de Mexico.
Results: We found 18.75% (3/16) with the C6667T mutation, the total of these patients were heterozygotes. The A1298C mutation was found in 50% (8/16) of the cases, from this total (1/8) were homozygotes and (7/8) heterozygotes.
Conclusions: We consider that is important quantify homocysteine in the preeclampsia investigation, with the purpose of finding associations between other enzymes of the homocysteine metabolism.
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