Hernández GCA, Bujalil ML, Jara DJ, Mier CJ, Bouchán VP

Language: Spanish
References: 15
Page: 20-28
PDF size: 93.54 Kb.

ABSTRACT

Objective: To evaluate the antioxidant intake and the lipoperoxidation status in plasma and peritoneal fluid of women with endometriosis.
Patients and methods: The study was carried out in 48 women with endometriosis obtained at the Sterility Clinic from the National Institute of Perinatology. The antioxidant intake was evaluated applying the Questionnaire Assessment of Antioxidants and Retinol Intakes in Mexican women validated by the National Institute of Public Health. The lipoperoxidation status was determined in plasma and peritoneal fluid with the malondyaldehyde method. Results were analyzed by ANOVA of Kruskal-Wallis.
Results: The antioxidant intake (vitamin C, vitamin E, selenium and zinc) of women with endometriosis showed a significative statistical difference when data was compared with the control group, including total group and those stratified in pathology stages. The lipoperoxidation status in plasma and peritoneal fluid of women with endometriosis, analyzed in group, did not show statistical difference versus healthy women. When data was stratified according to the pathology severity, the percentage of lipoperoxidation status increased in plasma in the severe grade of endometriosis and in peritoneal fluid in the moderate and severe grades.
Conclusions: The antioxidant intake in women with endometriosis showed an inverse correlation with the pathology intensity. As endometriosis severity intensifies, a less intake of antioxidants is present. There is a positive association between the pathology development and the lipoperoxidation status.

REFERENCES

1. Ramey J, Archer D. Peritoneal fluid: its relevance to the development of endometriosis. Fertil Steril 1993;60:1-13.

2. Hernández Guerrero C, Tlapanco Barba R, Ramos Pérez C, Velásquez Ramírez N, et al. Endometriosis y abatimiento de las características de la respuesta inmunológica citotóxica. Ginec Obst Mex 2003;71:559-74.

3. Hernández Guerrero C, Vadillo Ortega F, Beltrán Montoya J, Farina Granja M, et al. Inducción de la síntesis de óxido nítrico en células mononucleares en cultivo utilizando líquido peritoneal de mujeres con endometriosis, en relación con el porcentaje de linfocitos T y células NK identificado en dicho ambiente. Ginec Obst Mex 2001;69:12-23.

4. Honda R, Matsura K, Fukumatsu Y, et al. Peritoneal macrophages: a possible role in infertility associated with mild endometriosis. In: Minaguchi H, Sugimoto O, editors. Endometriosis. London: The Parthenon Publishing Group, 1997;pp:120-2.

5. Halme J. Role of peritoneal inflammation in endometriosis associated with infertility. Minaguchi H, Sugimoto O, editors. Endometriosis. London: The Parthenon Publishing Group 1997;pp:132-7.

6. Ota H, Igarashi S, Hatazawa J, Tanaka T. Endometriosis and free radicals. Gynecol Obstet Invest 1999;48:29-35.

7. Arumugam K, Dip Y. Endometriosis and infertility: the role of exogenous lipid peroxides in the peritoneal fluid. Fertil Steril 1995;63:198-9.

8. Combs G. Vitamins. 10th ed. Philadelphia: Saunders, 2000;pp:67-101.

9. Storey B. Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa. Mol Hum Reprod 1997;3:203-13

10. Rock JA. The revised American Fertility Society classification of endometriosis: reproducibility of scoring. Fertil Steril 1995;63:1108-10

11. Hernández-Ávila M, Romieu I, Parra S, Hernández-Ávila J, et al. Validity and reproducibility of a food frequency questionnaire to assess dietary intake of women living in Mexico City. Salud Pública de México 1998;39:133-40.

12. Bourges H, Casanueva E, Rosado JL. Recomendaciones de ingestión de nutrimentos para la población mexicana. Bases Fisiológicas. México: Panamericana, 2004.

13. Polak G, Rola R, Gogacz M, Koziol Montewka M, et al. Malondyaldehyde and total antioxidant status in the peritoneal fluid of infertile women. Ginekol Pol 1999;70:135-40.

14. Bendich A. Antioxidant micronutrients and immune responses. Ann NY Acad Sci 1990;587:168-80.

15. Bauer H, Jung T, Sikas D, Stichtenoth D, Frolich J, Neumann C. Nitric oxide inhibits the secretion of T-helper 1 and T-helper 2 associated cytokines of activated human T cells. Immunology 1997;90:205-11.