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2009, Number 2

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Rev Fac Med UNAM 2009; 52 (2)

Apoptosis and vascular endothelium in an experimental endocarditis

Domínguez-Hernández MA, Hernández CII, Sánchez ES, González HM, Galindo BJR, Baptiste MJ

Language: Spanish
References: 20
Page: 59-62
PDF size: 72.72 Kb.


ABSTRACT

Background: The pathophysiology of infective endocarditis involves a pathogen/host tissue interaction, leading to formation of thrombotic vegetations, related to bacteria-induced platelet activation. Phosphatidylserines residues are exposed by activated platelets, apoptotic and inflammatory cells. Since vegetations are platelet-fibrin clots in which platelet proaggregant activity is enhanced by bacterial colonization, we investigated the histological characteristics and apoptosis in vegetations using a model of infective endocarditis in rats. Methods and results: Nonbacterial and septic thrombotic endocarditis was induced in rats using a polyethylene catheter left indwelling through the aortic. The rats were injected with either a bacterial inoculum (Staphylococcus aureus) or saline. Histological analysis of myocardial tissue underlying the vegetations showed myocyte apoptosis and mucoid degeneration, without extracellular matrix degradation. Conclusions: The endocarditis experimental model presented can be used to study apoptosis, the endothelial injury process and the extracellular matrix degradation induced by germs.


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