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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2002, Number 4

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Rev Biomed 2002; 13 (4)

Comparative studio of three filters papers in neonatal screening UMELISA assays for phenylketonuria and congenital hypothyroidism

Frómeta-Suárez A, Marrero-González N, González-Reyes E, Lugo-Vallejo E, Fernández-Fernández L, Doménech-Mylnikova MB

Language: Spanish
References: 17
Page: 241-247
PDF size: 39.74 Kb.


ABSTRACT

Introduction. Newborn screening for congenital hypothyroidism and other inherited metabolic diseases share the fact that the analytes are determined in dried blood spotted on filter paper. The analyte concentration depends on the way the calibrators are prepared and other variables involved in this process like the lots and types of filter paper used, the matrix of calibrators, and the percentage of hematocrit among others.
Material and methods. Four controls prepared in total blood and hematocrit adjusted to 55% with different concentrations of TSH, T4 and Phe were evaluated. Each control was spotted on filter paper cards, Whatman BFC 180, Schleicher and Schuell 2992 and 903™. The analyte concentration was determined by UMELISA TSH Neonatal, UMELISA T4 Neonatal and UMTEST PKU. A stability study on the controls stored at 2-8 C for 9 months was performed. In addition 400 samples of neonates collected on filter papers 2992 and 903™ were evaluated, at the same time.
Results and conclusions. Student’s t test showed there were no significant differences between the 3 types of filter papers (p‹0.05) in that the precision and the accuracy of these methods weren't affected. The percentage of activity loss of analytes was less than 17%. The media concentrations in the distributions of TSH and Phe were slightly higher in samples collected on filter paper 903™.


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