Language: Spanish
References: 16
Page: 209-215
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ABSTRACT
Introduction. The frecuency of isolation of
Clostridium botulinum in Costa Rica, as well as in other countries is low; at the same time,
C. perfringens is the most common pathogenic species in nature. Because it has been described that
C. perfringens is able to inhibit the growth of
C. botulinum and the production of botulinal toxin, we decided to research on the effect that strains of
C. perfringens isolated from Costa Rican soils could have on the growth and toxin production of a strain of C. botulinum.
Procedure. Thirty-nine supernatants of strains of
C. perfringens from Costa Rican soils were tested for inhibition over the growth of
C. botulinum A (ATCC19399). Also, mixed cultures of C. botulinum and
C. perfringens were performed to evaluate for prevention of growth and toxin production.
Results. Fifty-eight percent of the strains of
C. perfringens showed an inhibitory effect on the growth of
C. botulinum; at least two strains completely inhibited the synthesis or the effect of botulinal toxin in the mixed cultures, even though
C. botulinum cells were isolated. The minimum number of necessary
C. perfringens cells to achieve this phenomenon was 10
8.
Conclusion. It is possible that the presence of
C. perfringens interferes with the isolation and confirmation of
C. botulinum in Costa Rican soils. It is still necessary to research more on the effects of native
C. perfringens over other groups and strains of
C. botulinum.
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